Construct: sgRNA BRDN0001146241
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGTCACTGATAAAACCGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTRK2 (4915)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76467
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
84745056 |
+ |
NTRK2 |
NNNTCACTGATAAAACCGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
83490792 |
+ |
SEMA3E |
NNNTCACTGATAAAACTGGT |
NGG |
1 |
0.4667 |
Tier II |
| 3 |
NC_000009.12 |
9 |
76683553 |
+ |
PRUNE2 |
NNNTCACTGATAAAACAGAT |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000006.12 |
6 |
139249532 |
- |
TXLNB |
NNNTCACTGATAACACCGGT |
NGG |
1 |
0.2143 |
Tier II |
| 5 |
NC_000004.12 |
4 |
10100762 |
+ |
WDR1 |
NNNTCACTGATAAAACAGGC |
NGG |
2 |
0.0424 |
Tier II |
| 6 |
NC_000010.11 |
10 |
78019535 |
+ |
POLR3A |
NNNTCACTGGTAAAACGGGT |
NGG |
2 |
0.0327 |
Tier II |
| 7 |
NC_000002.12 |
2 |
157859197 |
+ |
ACVR1 |
NNNTCACTGATAAAACAGGT |
NGC |
2 |
0.0104 |
Tier II |
| 8 |
NC_000011.10 |
11 |
31404161 |
- |
DNAJC24 |
NNNTCACTGATAAAACAGGT |
NGC |
2 |
0.0104 |
Tier II |
| 9 |
NC_000006.12 |
6 |
35003312 |
+ |
ANKS1A |
NNNTCACTGATAAAACCGGT |
NAA |
2 |
0.0 |
Tier II |
| 10 |
NC_000023.11 |
X |
43208510 |
- |
PINCR |
NNNACACTGATAAAAGCGGT |
NGG |
2 |
0.0733 |
Tier III |
| 11 |
NC_000010.11 |
10 |
6974751 |
+ |
LOC105376387 |
NNNTCACTGATAAAACCTGC |
NGG |
2 |
0.0606 |
Tier III |
| 12 |
NC_000002.12 |
2 |
53159484 |
+ |
LOC105369165 |
NNNTCACTGATAAAACAGGT |
NGC |
2 |
0.0104 |
Tier III |
| 13 |
NC_000002.12 |
2 |
157859197 |
+ |
LOC124907899 |
NNNTCACTGATAAAACAGGT |
NGC |
2 |
0.0104 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
66400033 |
- |
Lrrc6 |
NNNTCACTGATAAAACTGGT |
NGA |
2 |
0.0324 |
Tier II |
| 2 |
NC_000067.6 |
1 |
51568832 |
+ |
Gm17767 |
NNNTCACTGGTAAAACTGGT |
NGG |
2 |
0.2593 |
Tier III |
Other clones with same target sequence:
(none)
