Construct: sgRNA BRDN0001146242

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTACGAAGAGAACCGTGCCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPHK2 (56848)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77095

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 48626294 + SPHK2 NNNCGAAGAGAACCGTGCCG NGG 0 1.0 Tier I
2 NC_000011.10 11 2699789 + KCNQ1 NNNCGAGGAGAACCGCGCCG NGG 2 0.4706 Tier II
3 NC_000011.10 11 2699789 + KCNQ1OT1 NNNCGAGGAGAACCGCGCCG NGG 2 0.4706 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 66168121 + Dnah9 NNNCGCAGAGCACCGTGCCG NGG 2 0.1818 Tier I
2 NC_000076.6 10 93542162 - Ccdc38 NNNCGAAGACAACCGTGCCT NGG 2 0.28 Tier II
3 NC_000077.6 11 66168121 + Gm12296 NNNCGCAGAGCACCGTGCCG NGG 2 0.1818 Tier III
Download CSV

Other clones with same target sequence:

(none)