Construct: sgRNA BRDN0001146244
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACAGAAAGTATGTCCCGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKA2 (5256)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77842
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
18918729 |
+ |
PHKA2 |
NNNAGAAAGTATGTCCCGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
42025037 |
+ |
GLI3 |
NNNAGAAGGTATGTCCCGCA |
NGG |
2 |
0.6875 |
Tier II |
| 3 |
NC_000016.10 |
16 |
82163088 |
- |
MPHOSPH6 |
NNNAGAAAGTATGTCCCACC |
NGG |
2 |
0.2967 |
Tier II |
| 4 |
NC_000011.10 |
11 |
24581998 |
- |
LUZP2 |
NNNAGAAAGCATGTCCAGCG |
NGG |
2 |
0.2489 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
160564312 |
- |
Phka2 |
NNNAGAAAGTATGTCCCGAG |
NGG |
1 |
0.4286 |
Tier I |
| 2 |
NC_000083.6 |
17 |
31218496 |
+ |
Ubash3a |
NNNAGAAAGTATGTCCCACA |
NGG |
2 |
0.649 |
Tier II |
| 3 |
NC_000072.6 |
6 |
111321574 |
+ |
Grm7 |
NNNAGGAAGTATGTCCCTCG |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000081.6 |
15 |
80289156 |
- |
Cacna1i |
NNNAGAAAGTTTGTCCCGAG |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000074.6 |
8 |
129284916 |
+ |
Gm32856 |
NNNAGAAAGTATGTCCCGGT |
NGG |
2 |
0.0875 |
Tier II |
| 6 |
NC_000067.6 |
1 |
182632861 |
- |
Capn8 |
NNNAGAAAGTATGTCCCTGG |
NGG |
2 |
0.0833 |
Tier II |
| 7 |
NC_000074.6 |
8 |
25376022 |
+ |
Gm39146 |
NNNAGAAAGTATGTCCCTAG |
NGG |
2 |
0.2857 |
Tier III |
| 8 |
NC_000081.6 |
15 |
80289156 |
- |
Gm31319 |
NNNAGAAAGTTTGTCCCGAG |
NGG |
2 |
0.1319 |
Tier III |
| 9 |
NC_000078.6 |
12 |
109928014 |
- |
Gm34554 |
NNNAGAAAGTATGTCCCTGG |
NGG |
2 |
0.0833 |
Tier III |
| 10 |
NC_000073.6 |
7 |
39664531 |
- |
Gm2021 |
NNNAGAAAGTATGTCCCTCG |
NGT |
2 |
0.0108 |
Tier III |
Other clones with same target sequence:
(none)
