Construct: sgRNA BRDN0001146251

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCATTTGGTCAAAAATGGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK1 (4750)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76125

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 169556019 + NEK1 NNNTTTGGTCAAAAATGGCA NGG 0 1.0 Tier I
2 NC_000001.11 1 76345845 - ST6GALNAC3 NNNTTTGATCAAAAATAGCA NGG 2 0.9333 Tier II
3 NC_000005.10 5 148408810 + FBXO38 NNNTTTGGCCAGAAATGGCA NGG 2 0.6667 Tier II
4 NC_000005.10 5 129960319 + CHSY3 NNNTTTGGCCAAAAATGACA NGG 2 0.6391 Tier II
5 NC_000015.10 15 68577438 + CORO2B NNNTGTGGTTAAAAATGGCA NGG 2 0.6024 Tier II
6 NC_000003.12 3 180930675 + FXR1 NNNTTTGTTAAAAAATGGCA NGG 2 0.5417 Tier II
7 NC_000012.12 12 7790208 + NANOG NNNTTTTGCCAAAAATGGCA NGG 2 0.5275 Tier II
8 NC_000004.12 4 143469204 - GAB1 NNNTTTGTTCAGAAATGGCA NGG 2 0.4514 Tier II
9 NC_000004.12 4 38073642 - TBC1D1 NNNTTTGGTCAAAAATGGAA NGG 1 0.4286 Tier II
10 NC_000012.12 12 15461301 - PTPRO NNNTTTGGTCCAAAATGGCA NGG 1 0.4 Tier II
11 NC_000007.14 7 39186571 - POU6F2 NNNTTTGGGCAAAAATGGCT NGG 2 0.3714 Tier II
12 NC_000010.11 10 6247312 + PFKFB3 NNNCTTGGTCAAAAATGGAA NGG 2 0.2773 Tier II
13 NC_000018.10 18 21761697 + MIB1 NNNTTTGGTCACAAAAGGCA NGG 2 0.2392 Tier II
14 NC_000014.9 14 29722911 + PRKD1 NNNTATGGTCAAAAATGGTA NGG 2 0.2308 Tier II
15 NC_000016.10 16 24334769 + CACNG3 NNNTTTGGCCAAAATTGGCA NGG 2 0.1846 Tier II
16 NC_000009.12 9 27184749 + TEK NNNATTGGTCAAAAATGGCA NAG 2 0.1235 Tier II
17 NC_000002.12 2 160113334 + ITGB6 NNNTTTGGTCAAAAATGCCA NAG 2 0.1235 Tier II
18 NC_000010.11 10 15822027 - MINDY3 NNNTTTTGTCAACAATGGCA NGG 2 0.1203 Tier II
19 NC_000023.11 X 41561121 + CASK NNNTATGGTCAAAAATGGCC NGG 2 0.1136 Tier II
20 NC_000011.10 11 72837349 + FCHSD2 NNNTTTGGTCAAAAATCCCA NGG 2 0.112 Tier II
21 NC_000011.10 11 72837349 + ATG16L2 NNNTTTGGTCAAAAATCCCA NGG 2 0.112 Tier II
22 NC_000001.11 1 2244844 - SKI NNNTTTGGTCACCAATGGCA NGG 2 0.0554 Tier II
23 NC_000004.12 4 86913050 + C4orf36 NNNTTTGGTCAAAAATGGAA NGA 2 0.0298 Tier II
24 NC_000013.11 13 51686538 - WDFY2 NNNTTTTGTCAAAAATGGCA NGC 2 0.0127 Tier II
25 NC_000012.12 12 47086685 + PCED1B NNNTTTGGTCATAAATGGCA NGT 2 0.0054 Tier II
26 NC_000023.11 X 124021598 + STAG2 NNNTTTGGTCAAAAATGGCA NTC 2 0.0 Tier II
27 NC_000012.12 12 7893478 + NANOGP1 NNNTTTTGCCAAAAATGGCA NGG 2 0.5275 Tier III
28 NC_000011.10 11 122120925 + MIR100HG NNNTTTGGACAAAAATGCCA NGG 2 0.4422 Tier III
29 NC_000001.11 1 168408559 + LOC100505918 NNNTTTGGGCAAAAATGTCA NGG 2 0.4127 Tier III
30 NC_000001.11 1 168408559 + LOC125312414 NNNTTTGGGCAAAAATGTCA NGG 2 0.4127 Tier III
31 NC_000012.12 12 106564110 + LOC100287944 NNNTGTGGTCAAAAATGGAA NGG 2 0.2743 Tier III
32 NC_000013.11 13 91171982 - LINC00379 NNNTTTGGTCCAGAATGGCA NGG 2 0.2609 Tier III
33 NC_000010.11 10 106109927 + LOC105378469 NNNTTTCGTCTAAAATGGCA NGG 2 0.2115 Tier III
34 NC_000008.11 8 117234726 + LOC105375716 NNNTTGGGTCATAAATGGCA NGG 2 0.1905 Tier III
35 NC_000019.10 19 6975583 - ADGRE4P NNNTTTGGTCAAAAATGGGA NGG 1 0.125 Tier III
36 NC_000010.11 10 15822027 - LOC124902383 NNNTTTTGTCAACAATGGCA NGG 2 0.1203 Tier III
37 NC_000007.14 7 38367224 - TRG NNNTTTGGACAAAAATGGGA NGG 2 0.1161 Tier III
38 NC_000007.14 7 38367224 - TRG-AS1 NNNTTTGGACAAAAATGGGA NGG 2 0.1161 Tier III
39 NC_000013.11 13 87459523 + MIR4500HG NNNTTTGCTCAAAAATGGGA NGG 2 0.0769 Tier III
40 NC_000007.14 7 119671286 - LINC02476 NNNTTTGGTCAATAATTGCA NGG 2 0.075 Tier III
41 NC_000004.12 4 86913050 + ARPC1AP4 NNNTTTGGTCAAAAATGGAA NGA 2 0.0298 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 61049861 - Nek1 NNNTTTGGTCAAAAATGGCA NGG 0 1.0 Tier I
2 NC_000079.6 13 20905698 - Aoah NNNCTTGGTCAAAAATGGTA NGG 2 0.2986 Tier I
3 NC_000069.6 3 130630730 - Etnppl NNNTTTAGTTAAAAATGGCA NGG 2 0.9412 Tier II
4 NC_000070.6 4 40728602 - Dnaja1 NNNTTTGGTCAAAAAAGACA NGG 2 0.6294 Tier II
5 NC_000076.6 10 36769007 - Hs3st5 NNNTTTGGTTGAAAATGGCA NGG 2 0.6118 Tier II
6 NC_000070.6 4 3588995 - Tgs1 NNNTTTGGTCAAAGATGGCA NGG 1 0.4667 Tier II
7 NC_000074.6 8 13645466 - Rasa3 NNNTTTGCTCAGAAATGGCA NGG 2 0.4444 Tier II
8 NC_000068.7 2 73881094 + Atf2 NNNATTGGCCAAAAATGGCA NGG 2 0.4396 Tier II
9 NC_000079.6 13 17267538 - Sugct NNNTTTGGTCAAAGATAGCA NGG 2 0.4356 Tier II
10 NC_000085.6 19 56795271 + Ccdc186 NNNCTTGGGCAAAAATGGCA NGG 2 0.4006 Tier II
11 NC_000070.6 4 53703181 + Fsd1l NNNTTTTGTCAAAAATGACA NGG 2 0.3956 Tier II
12 NC_000081.6 15 44637641 + Ebag9 NNNTTTGGTCAAAAAAGGAA NGG 2 0.3896 Tier II
13 NC_000069.6 3 96618163 - Lix1l NNNTTTAGTGAAAAATGGCA NGG 2 0.3889 Tier II
14 NC_000072.6 6 77970447 + Ctnna2 NNNTTTGTTCAAAAATGGAA NGG 2 0.2679 Tier II
15 NC_000082.6 16 41523117 - Lsamp NNNTTTGGTCAAAAATTGCA NGG 1 0.25 Tier II
16 NC_000081.6 15 10674777 - Rai14 NNNTTTGGTCAAAAATGCCA NAG 2 0.1235 Tier II
17 NC_000071.6 5 145108636 - Arpc1a NNNTTTGGTCAAAAATGGAA NGA 2 0.0298 Tier II
18 NC_000069.6 3 58786750 + 4930593A02Rik NNNTTTGGTCAAAAATGACA NGG 1 0.6923 Tier III
19 NC_000069.6 3 96618163 - 6330549D23Rik NNNTTTAGTGAAAAATGGCA NGG 2 0.3889 Tier III
20 NC_000080.6 14 98927135 + Gm41228 NNNTTTGGTGAAAAATGGCA NGA 2 0.027 Tier III
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Other clones with same target sequence:

(none)