Construct: sgRNA BRDN0001146253
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGGACTGCATAGTGCTAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS1L1 (221823)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75839
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
18027163 |
- |
PRPS1L1 |
NNNGACTGCATAGTGCTAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
14307905 |
- |
NFIB |
NNNTACTGCATAGTGTTAGT |
NGG |
2 |
0.1119 |
Tier I |
| 3 |
NC_000006.12 |
6 |
138673895 |
+ |
NHSL1 |
NNNGACTGCATAGTGCAAGT |
NGG |
1 |
0.5333 |
Tier II |
| 4 |
NC_000003.12 |
3 |
14964392 |
- |
NR2C2 |
NNNGACTGCATAGTGGTAGG |
NGG |
2 |
0.0271 |
Tier II |
| 5 |
NC_000014.9 |
14 |
106339505 |
- |
IGH |
NNNGATTCCATAGTGCTAGT |
NGG |
2 |
0.5714 |
Tier III |
| 6 |
NC_000014.9 |
14 |
106364218 |
- |
IGH |
NNNGATTCCATAGTGCTAGT |
NGG |
2 |
0.5714 |
Tier III |
| 7 |
NC_000014.9 |
14 |
106859516 |
- |
IGH |
NNNGATTCCATAGTGCTAGT |
NGG |
2 |
0.5714 |
Tier III |
| 8 |
NC_000014.9 |
14 |
106859516 |
- |
LOC124903399 |
NNNGATTCCATAGTGCTAGT |
NGG |
2 |
0.5714 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
113335458 |
- |
Btbd10 |
NNNGTCTGCATGGTGCTAGT |
NGG |
2 |
0.2626 |
Tier I |
| 2 |
NC_000070.6 |
4 |
126196107 |
- |
Thrap3 |
NNNGACTAAATAGTGCTAGT |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000076.6 |
10 |
125987474 |
+ |
Lrig3 |
NNNGACGGCATGGTGCTAGT |
NGG |
2 |
0.4248 |
Tier II |
| 4 |
NC_000068.7 |
2 |
53104988 |
- |
Fmnl2 |
NNNGACTACATAGTGCTGGT |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000074.6 |
8 |
46063269 |
- |
Snx25 |
NNNGACTGCAGAGTGCTAGT |
NGG |
1 |
0.4 |
Tier II |
| 6 |
NC_000070.6 |
4 |
82499149 |
- |
Nfib |
NNNTACTGCATAGTGCTACT |
NGG |
2 |
0.163 |
Tier II |
| 7 |
NC_000080.6 |
14 |
48446167 |
+ |
Tmem260 |
NNNGACTGCATAGTGCGAGT |
NGG |
1 |
0.1176 |
Tier II |
| 8 |
NC_000081.6 |
15 |
76674084 |
+ |
Ppp1r16a |
NNNTACTGCATAGTCCTAGT |
NGG |
2 |
0.0992 |
Tier II |
Other clones with same target sequence:
(none)
