Construct: sgRNA BRDN0001146255

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTAAACGACACCTTATTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80237

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 118239971 + ANKRD7 NNNAATAGACACCTTATTCT NGG 2 0.5357 Tier I
2 NC_000005.10 5 20373520 - CDH18 NNNAAACAACACCTTACTCT NGG 2 0.7059 Tier II
3 NC_000002.12 2 99805998 + AFF3 NNNAAATGACAACTTATTCT NGG 2 0.5804 Tier II
4 NC_000003.12 3 113651509 - USF3 NNNAAACGTAACCTTATTCT NGG 2 0.52 Tier II
5 NC_000001.11 1 77176616 - PIGK NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
6 NC_000003.12 3 126194023 + ALDH1L1 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
7 NC_000003.12 3 133793766 - TF NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
8 NC_000003.12 3 133793766 - SRPRB NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
9 NC_000004.12 4 142390889 - INPP4B NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
10 NC_000006.12 6 118192476 + SLC35F1 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
11 NC_000011.10 11 35695625 + TRIM44 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
12 NC_000018.10 18 13704947 - FAM210A NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
13 NC_000022.11 22 17406188 - CECR2 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
14 NC_000023.11 X 64954619 - ZC4H2 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier II
15 NC_000001.11 1 3858138 + DFFB NNNAATCGTCACCTTATTCT NGG 2 0.4286 Tier II
16 NC_000010.11 10 106601401 + SORCS1 NNNAAACAACACATTATTCT NGG 2 0.3846 Tier II
17 NC_000011.10 11 60025829 - OOSP4B NNNAAAGGACACCTTATTTT NGG 2 0.2172 Tier II
18 NC_000012.12 12 43452923 + ADAMTS20 NNNAAACGATACCTTATTCT NGA 2 0.0654 Tier II
19 NC_000009.12 9 77346935 - VPS13A NNNAAAGGACACCTTATTCT NGT 2 0.0076 Tier II
20 NC_000006.12 6 168610993 + SMOC2 NNNAAACCACACCTTCTTCT NGG 2 0.0 Tier II
21 NC_000002.12 2 52178603 - NRXN1-DT NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier III
22 NC_000003.12 3 126194023 + ALDH1L1-AS2 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier III
23 NC_000003.12 3 133793766 - LOC105374116 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier III
24 NC_000008.11 8 47066632 - LOC105375815 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier III
25 NC_000008.11 8 116070930 - LINC00536 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier III
26 NC_000015.10 15 26090697 + LOC105370739 NNNAAAAGACACCATATTCT NGG 2 0.4643 Tier III
27 NC_000008.11 8 16661461 + LOC101929028 NNNAAACGCCACCTAATTCT NGG 2 0.3308 Tier III
28 NC_000009.12 9 99593519 - LOC101928438 NNNAAACGTCAGCTTATTCT NGG 2 0.2667 Tier III
29 NC_000004.12 4 99983250 - H2AZ1-DT NNNAAACCACACCTTATTCT NAG 2 0.1595 Tier III
30 NC_000003.12 3 958114 - LOC107986059 NNNAAACGACACCTTGATCT NGG 2 0.1026 Tier III
31 NC_000006.12 6 113173876 - LOC105377953 NNNAAACGACACCTTGATCT NGG 2 0.1026 Tier III
32 NC_000006.12 6 113173876 - LOC105377955 NNNAAACGACACCTTGATCT NGG 2 0.1026 Tier III
33 NC_000023.11 X 111618189 - EIF4BP7 NNNAAACGACACCTTGATCT NGG 2 0.1026 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 26873462 - Fbxl7 NNNAAATGACACTTTATTCT NGG 2 0.5688 Tier II
2 NC_000072.6 6 99676573 - Eif4e3 NNNACACGACACCTTATACT NGG 2 0.3333 Tier II
3 NC_000082.6 16 65084667 + Htr1f NNNAAATGACACATTATTCT NGG 2 0.3125 Tier II
4 NC_000076.6 10 116070109 + Ptprr NNNCAAAGACACCTTATTCT NGG 2 0.2647 Tier II
5 NC_000068.7 2 3227403 + Fam171a1 NNNAAACGATACCTTGTTCT NGG 2 0.181 Tier II
6 NC_000075.6 9 119244118 + Oxsr1 NNNAAACGACACCTTGTTCC NGG 2 0.0175 Tier II
7 NC_000086.7 X 155966526 - Gm15155 NNNAAATTACACCTTATTCT NGG 2 0.5078 Tier III
8 NC_000072.6 6 99676573 - Gm52869 NNNACACGACACCTTATACT NGG 2 0.3333 Tier III
9 NC_000077.6 11 96987804 - Gm51916 NNNAAACCACACCTGATTCT NGG 2 0.0308 Tier III
10 NC_000073.6 7 94587303 + Gm32647 NNNAAACGACACCTTCATCT NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)

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