GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001146255

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: ATTAAACGACACCTTATTCT

Original Target:

Taxon:: Homo sapiens (human)
Gene:: NO_SITE control
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 80237

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000007.14	7	118239971	+	ANKRD7	NNNAATAGACACCTTATTCT	NGG	2	0.5357	Tier I
2	NC_000005.10	5	20373520	-	CDH18	NNNAAACAACACCTTACTCT	NGG	2	0.7059	Tier II
3	NC_000002.12	2	99805998	+	AFF3	NNNAAATGACAACTTATTCT	NGG	2	0.5804	Tier II
4	NC_000003.12	3	113651509	-	USF3	NNNAAACGTAACCTTATTCT	NGG	2	0.52	Tier II
5	NC_000001.11	1	77176616	-	PIGK	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
6	NC_000003.12	3	126194023	+	ALDH1L1	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
7	NC_000003.12	3	133793766	-	TF	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
8	NC_000003.12	3	133793766	-	SRPRB	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
9	NC_000004.12	4	142390889	-	INPP4B	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
10	NC_000006.12	6	118192476	+	SLC35F1	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
11	NC_000011.10	11	35695625	+	TRIM44	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
12	NC_000018.10	18	13704947	-	FAM210A	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
13	NC_000022.11	22	17406188	-	CECR2	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
14	NC_000023.11	X	64954619	-	ZC4H2	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier II
15	NC_000001.11	1	3858138	+	DFFB	NNNAATCGTCACCTTATTCT	NGG	2	0.4286	Tier II
16	NC_000010.11	10	106601401	+	SORCS1	NNNAAACAACACATTATTCT	NGG	2	0.3846	Tier II
17	NC_000011.10	11	60025829	-	OOSP4B	NNNAAAGGACACCTTATTTT	NGG	2	0.2172	Tier II
18	NC_000012.12	12	43452923	+	ADAMTS20	NNNAAACGATACCTTATTCT	NGA	2	0.0654	Tier II
19	NC_000009.12	9	77346935	-	VPS13A	NNNAAAGGACACCTTATTCT	NGT	2	0.0076	Tier II
20	NC_000006.12	6	168610993	+	SMOC2	NNNAAACCACACCTTCTTCT	NGG	2	0.0	Tier II
21	NC_000002.12	2	52178603	-	NRXN1-DT	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier III
22	NC_000003.12	3	126194023	+	ALDH1L1-AS2	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier III
23	NC_000003.12	3	133793766	-	LOC105374116	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier III
24	NC_000008.11	8	47066632	-	LOC105375815	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier III
25	NC_000008.11	8	116070930	-	LINC00536	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier III
26	NC_000015.10	15	26090697	+	LOC105370739	NNNAAAAGACACCATATTCT	NGG	2	0.4643	Tier III
27	NC_000008.11	8	16661461	+	LOC101929028	NNNAAACGCCACCTAATTCT	NGG	2	0.3308	Tier III
28	NC_000009.12	9	99593519	-	LOC101928438	NNNAAACGTCAGCTTATTCT	NGG	2	0.2667	Tier III
29	NC_000004.12	4	99983250	-	H2AZ1-DT	NNNAAACCACACCTTATTCT	NAG	2	0.1595	Tier III
30	NC_000003.12	3	958114	-	LOC107986059	NNNAAACGACACCTTGATCT	NGG	2	0.1026	Tier III
31	NC_000006.12	6	113173876	-	LOC105377953	NNNAAACGACACCTTGATCT	NGG	2	0.1026	Tier III
32	NC_000006.12	6	113173876	-	LOC105377955	NNNAAACGACACCTTGATCT	NGG	2	0.1026	Tier III
33	NC_000023.11	X	111618189	-	EIF4BP7	NNNAAACGACACCTTGATCT	NGG	2	0.1026	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000081.6	15	26873462	-	Fbxl7	NNNAAATGACACTTTATTCT	NGG	2	0.5688	Tier II
2	NC_000072.6	6	99676573	-	Eif4e3	NNNACACGACACCTTATACT	NGG	2	0.3333	Tier II
3	NC_000082.6	16	65084667	+	Htr1f	NNNAAATGACACATTATTCT	NGG	2	0.3125	Tier II
4	NC_000076.6	10	116070109	+	Ptprr	NNNCAAAGACACCTTATTCT	NGG	2	0.2647	Tier II
5	NC_000068.7	2	3227403	+	Fam171a1	NNNAAACGATACCTTGTTCT	NGG	2	0.181	Tier II
6	NC_000075.6	9	119244118	+	Oxsr1	NNNAAACGACACCTTGTTCC	NGG	2	0.0175	Tier II
7	NC_000086.7	X	155966526	-	Gm15155	NNNAAATTACACCTTATTCT	NGG	2	0.5078	Tier III
8	NC_000072.6	6	99676573	-	Gm52869	NNNACACGACACCTTATACT	NGG	2	0.3333	Tier III
9	NC_000077.6	11	96987804	-	Gm51916	NNNAAACCACACCTGATTCT	NGG	2	0.0308	Tier III
10	NC_000073.6	7	94587303	+	Gm32647	NNNAAACGACACCTTCATCT	NGG	2	0.0	Tier III

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Other clones with same target sequence:

(none)