Construct: sgRNA BRDN0001146259
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCGCTCTCAATTGCTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR1C (130399)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76522
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
157544489 |
- |
ACVR1C |
NNNGCGCTCTCAATTGCTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
13790251 |
+ |
PRDM2 |
NNNTAGCTCTCAATTGCTAG |
NGG |
2 |
0.2078 |
Tier II |
| 3 |
NC_000006.12 |
6 |
163103321 |
+ |
PACRG |
NNNGAGCTCTCAACTGCTAG |
NGG |
2 |
0.1633 |
Tier II |
| 4 |
NC_000006.12 |
6 |
85921296 |
+ |
LOC101928842 |
NNNGCTCTCACAATTGCTAG |
NGG |
2 |
0.5714 |
Tier III |
| 5 |
NC_000006.12 |
6 |
77172618 |
+ |
LOC101928570 |
NNNGCACTCTCATTTGCTAG |
NGG |
2 |
0.3 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
34574233 |
- |
Nme5 |
NNNGCTCTCTCATTTGCTAG |
NGG |
2 |
0.2 |
Tier II |
| 2 |
NC_000068.7 |
2 |
58941725 |
- |
Ccdc148 |
NNNGGGCTCTCAACTGCTAG |
NGG |
2 |
0.1714 |
Tier II |
| 3 |
NC_000075.6 |
9 |
27348755 |
+ |
Igsf9b |
NNNGCGATCTCAATTGCTAG |
NTG |
2 |
0.0292 |
Tier II |
| 4 |
NC_000076.6 |
10 |
24531286 |
- |
Gm15270 |
NNNGTGCTCTCAATTGCTAG |
NTG |
2 |
0.0248 |
Tier III |
| 5 |
NC_000086.7 |
X |
78407969 |
- |
Gm14752 |
NNNGCGCTCTGAATTTCTAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
