Construct: sgRNA BRDN0001146260
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACTGCCCTTAACACCAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MINK1 (50488)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76260
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000077.6 |
11 |
70608384 |
+ |
Mink1 |
NNNTGCCCTTAACACCAGTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000073.6 |
7 |
16549130 |
- |
Arhgap35 |
NNNTGCCCTTAACAACAGTG |
NTG |
2 |
0.0087 |
Tier I |
3 |
NC_000083.6 |
17 |
70673758 |
- |
Dlgap1 |
NNNTGCCTGTAACACCAGTG |
NGG |
2 |
0.5417 |
Tier II |
4 |
NC_000083.6 |
17 |
10798796 |
+ |
Pacrg |
NNNTGCCCTTAACTCAAGTG |
NGG |
2 |
0.5333 |
Tier II |
5 |
NC_000067.6 |
1 |
175097159 |
+ |
Rgs7 |
NNNTCCCCTTAACACCACTG |
NGG |
2 |
0.3741 |
Tier II |
6 |
NC_000073.6 |
7 |
98060906 |
- |
Myo7a |
NNNTGCCATTAAAACCAGTG |
NGG |
2 |
0.25 |
Tier II |
7 |
NC_000079.6 |
13 |
103184107 |
- |
Mast4 |
NNNTGCTCTTAACACCAGTG |
NAG |
2 |
0.2106 |
Tier II |
8 |
NC_000080.6 |
14 |
30967742 |
+ |
Nek4 |
NNNTGCCTTTAACCCCAGTG |
NGG |
2 |
0.1875 |
Tier II |
9 |
NC_000082.6 |
16 |
22516280 |
+ |
Dgkg |
NNNGGCCCTTACCACCAGTG |
NGG |
2 |
0.1645 |
Tier II |
10 |
NC_000079.6 |
13 |
64099240 |
- |
Slc35d2 |
NNNTGCGCTTAACACCAGGG |
NGG |
2 |
0.1176 |
Tier II |
11 |
NC_000070.6 |
4 |
99203062 |
+ |
Atg4c |
NNNTGCCCTCAACACCTGTG |
NGG |
2 |
0.0711 |
Tier II |
12 |
NC_000072.6 |
6 |
91400340 |
+ |
Wnt7a |
NNNTGCCCTGAAGACCAGTG |
NGG |
2 |
0.0682 |
Tier II |
13 |
NC_000075.6 |
9 |
89966565 |
+ |
Rasgrf1 |
NNNTGCCTTTAACACCAGTG |
NTG |
2 |
0.0341 |
Tier II |
14 |
NC_000074.6 |
8 |
124598092 |
+ |
Capn9 |
NNNTGCCCTCAACACCAGTG |
NTG |
2 |
0.0208 |
Tier II |
15 |
NC_000068.7 |
2 |
144139149 |
+ |
Banf2os |
NNNTGCCAATAACACCAGTG |
NGG |
2 |
0.6036 |
Tier III |
16 |
NC_000083.6 |
17 |
70673758 |
- |
Gm36746 |
NNNTGCCTGTAACACCAGTG |
NGG |
2 |
0.5417 |
Tier III |
17 |
NC_000082.6 |
16 |
22516280 |
+ |
9230117E06Rik |
NNNGGCCCTTACCACCAGTG |
NGG |
2 |
0.1645 |
Tier III |
18 |
NC_000068.7 |
2 |
92810637 |
+ |
Gm32413 |
NNNTGCCCTTACCAGCAGTG |
NGG |
2 |
0.0132 |
Tier III |
19 |
NC_000068.7 |
2 |
92810637 |
+ |
Gm39880 |
NNNTGCCCTTACCAGCAGTG |
NGG |
2 |
0.0132 |
Tier III |
Other clones with same target sequence:
(none)