Construct: sgRNA BRDN0001146263
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTGTTGCTTAGTAACCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GAK (2580)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76471
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
898135 |
- |
GAK |
NNNTGTTGCTTAGTAACCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
84871542 |
+ |
DLG2 |
NNNTGTTGCTAAATAACCAA |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000020.11 |
20 |
43918286 |
+ |
TOX2 |
NNNTGTTGCTTAGTGACCTA |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000010.11 |
10 |
37795286 |
+ |
ZNF248 |
NNNTGTTGATGAGTAACCAA |
NGG |
2 |
0.3429 |
Tier II |
| 5 |
NC_000009.12 |
9 |
20928733 |
+ |
FOCAD |
NNNTATTGCTTTGTAACCAA |
NGG |
2 |
0.2889 |
Tier II |
| 6 |
NC_000002.12 |
2 |
173109300 |
- |
MAP3K20 |
NNNTATTGCTTAGTAACCAA |
NAG |
2 |
0.2247 |
Tier II |
| 7 |
NC_000024.10 |
Y |
2965989 |
- |
ZFY |
NNNTGTTGCTTAGTAAACAA |
NAG |
2 |
0.121 |
Tier II |
| 8 |
NC_000011.10 |
11 |
118757060 |
- |
DDX6 |
NNNTGTTCCTTAGTAGCCAA |
NGG |
2 |
0.1183 |
Tier II |
| 9 |
NC_000007.14 |
7 |
71417046 |
+ |
GALNT17 |
NNNTGTTGCTAAGTAACCAA |
NGA |
2 |
0.0521 |
Tier II |
| 10 |
NC_000003.12 |
3 |
60156011 |
+ |
FHIT |
NNNTGTTGCTTAGTAACCAT |
NGC |
2 |
0.0133 |
Tier II |
| 11 |
NC_000007.14 |
7 |
102152984 |
+ |
CUX1 |
NNNAGTTGCTTAGTAACCAA |
NGC |
2 |
0.0106 |
Tier II |
| 12 |
NC_000001.11 |
1 |
222915904 |
+ |
DISP1 |
NNNTGTTGCTTTGTAACCAA |
NGT |
2 |
0.0054 |
Tier II |
| 13 |
NC_000001.11 |
1 |
77539756 |
- |
AK5 |
NNNTGTTACTTAGGAACCAA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000002.12 |
2 |
144954149 |
+ |
TEX41 |
NNNTGGTGCTTAGTAACCAG |
NGG |
2 |
0.437 |
Tier III |
| 15 |
NC_000003.12 |
3 |
191477688 |
- |
PYDC2-AS1 |
NNNTGCTGCTTAGTAAACAA |
NGG |
2 |
0.4242 |
Tier III |
| 16 |
NC_000007.14 |
7 |
69172870 |
- |
LOC105375344 |
NNNTGTTGCCTAGCAACCAA |
NGG |
2 |
0.1524 |
Tier III |
| 17 |
NC_000004.12 |
4 |
84002763 |
- |
LINC02994 |
NNNTGTTGCTTAGTAAGAAA |
NGG |
2 |
0.0317 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
5260614 |
- |
Syne1 |
NNNTGATGCTTAGAAACCAA |
NGG |
2 |
0.5365 |
Tier II |
| 2 |
NC_000082.6 |
16 |
31895710 |
- |
Meltf |
NNNTGTTGAGTAGTAACCAA |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000081.6 |
15 |
93447729 |
- |
Pphln1 |
NNNTGTTTCTTAGAAACCAA |
NGG |
2 |
0.3869 |
Tier II |
| 4 |
NC_000068.7 |
2 |
49533873 |
+ |
Epc2 |
NNNTGTTGCTCAGTAAACAA |
NGG |
2 |
0.3111 |
Tier II |
| 5 |
NC_000067.6 |
1 |
19120397 |
- |
Tfap2d |
NNNTTTTGCTTAGTAACCAA |
NGG |
1 |
0.3 |
Tier II |
| 6 |
NC_000073.6 |
7 |
16959662 |
+ |
Pnmal1 |
NNNTGATGCTTAGCAACCAA |
NGG |
2 |
0.2476 |
Tier II |
| 7 |
NC_000068.7 |
2 |
145487613 |
- |
Slc24a3 |
NNNTGTTTCTTTGTAACCAA |
NGG |
2 |
0.2083 |
Tier II |
| 8 |
NC_000081.6 |
15 |
55123111 |
+ |
Deptor |
NNNTGTTGCTTATTAACCAT |
NGG |
2 |
0.18 |
Tier II |
| 9 |
NC_000079.6 |
13 |
114289132 |
+ |
Ndufs4 |
NNNTCTTGCTTAGTTACCAA |
NGG |
2 |
0.1571 |
Tier II |
| 10 |
NC_000086.7 |
X |
85084809 |
+ |
Dmd |
NNNTGTTGCTTAGAAAGCAA |
NGG |
2 |
0.0364 |
Tier II |
| 11 |
NC_000076.6 |
10 |
73214576 |
- |
Pcdh15 |
NNNTATTGCTTAGTAACCAA |
NGC |
2 |
0.0193 |
Tier II |
| 12 |
NC_000068.7 |
2 |
43910830 |
- |
Arhgap15 |
NNNTGTTGCTTAGTAACCCA |
NTG |
2 |
0.0081 |
Tier II |
| 13 |
NC_000081.6 |
15 |
10670054 |
- |
Rai14 |
NNNTGTTGCTAAGGAACCAA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000071.6 |
5 |
149398705 |
+ |
Medag |
NNNTGTTGCTTAGTATCCAA |
NTG |
2 |
0.0 |
Tier II |
| 15 |
NC_000076.6 |
10 |
9025327 |
+ |
Gm51794 |
NNNTGTTGATAAGTAACCAA |
NGG |
2 |
0.6429 |
Tier III |
| 16 |
NC_000086.7 |
X |
5737607 |
+ |
Gm39489 |
NNNTGATGCCTAGTAACCAA |
NGG |
2 |
0.4622 |
Tier III |
| 17 |
NC_000082.6 |
16 |
31895710 |
- |
Gm52243 |
NNNTGTTGAGTAGTAACCAA |
NGG |
2 |
0.4286 |
Tier III |
| 18 |
NC_000067.6 |
1 |
151302081 |
- |
Gm10138 |
NNNTGTTGCTTAGCAACCAG |
NGG |
2 |
0.2185 |
Tier III |
| 19 |
NC_000085.6 |
19 |
30491117 |
+ |
Gm17885 |
NNNTGTTCCTTAGTCACCAA |
NGG |
2 |
0.1678 |
Tier III |
Other clones with same target sequence:
(none)
