Construct: sgRNA BRDN0001146264

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGTCAAGTGATCAGTGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2H1 (2965)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76600

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 18338177 + GTF2H1 NNNTCAAGTGATCAGTGCTG NGG 0 1.0 Tier I
2 NC_000003.12 3 38002586 - VILL NNNTCAAGTGGTCAGTGCTG NGG 1 0.65 Tier I
3 NC_000015.10 15 77686838 - LINGO1 NNNCCAAGTGATCAGAGCTG NGG 2 0.5882 Tier II
4 NC_000005.10 5 116019873 + LVRN NNNTGAAGTGACCAGTGCTG NGG 2 0.5684 Tier II
5 NC_000002.12 2 20212191 - SDC1 NNNTCTGGTGATCAGTGCTG NGG 2 0.5042 Tier II
6 NC_000012.12 12 3671318 + CRACR2A NNNCCAGGTGATCAGTGCTG NGG 2 0.4567 Tier II
7 NC_000021.9 21 44400580 - TRPM2 NNNTCATGAGATCAGTGCTG NGG 2 0.4062 Tier II
8 NC_000015.10 15 76613668 - SCAPER NNNTCTAGTGATCAGTGATG NGG 2 0.3846 Tier II
9 NC_000020.11 20 14183791 + MACROD2 NNNTCTAGTGATCAGTGATG NGG 2 0.3846 Tier II
10 NC_000010.11 10 5966051 - IL15RA NNNTCAAGGGATCTGTGCTG NGG 2 0.3302 Tier II
11 NC_000007.14 7 121364306 + FAM3C NNNTCAATTGTTCAGTGCTG NGG 2 0.1923 Tier II
12 NC_000008.11 8 101632036 - GRHL2 NNNTCAAGTGAGCACTGCTG NGG 2 0.1364 Tier II
13 NC_000012.12 12 52827272 - KRT79 NNNTCAAGTGATGAGAGCTG NGG 2 0.124 Tier II
14 NC_000012.12 12 101079737 + ANO4 NNNTCAAGTGTTCCGTGCTG NGG 2 0.0659 Tier II
15 NC_000003.12 3 57603583 + PDE12 NNNTCAAGTGATCTGTGCTG NGA 2 0.037 Tier II
16 NC_000014.9 14 61403674 + PRKCH NNNACAAGTGATCAGTGCTG NGA 2 0.0331 Tier II
17 NC_000010.11 10 7374764 - SFMBT2 NNNTCAAGTCATCAGTGCTG NGC 2 0.0089 Tier II
18 NC_000002.12 2 20212191 - LOC124905978 NNNTCTGGTGATCAGTGCTG NGG 2 0.5042 Tier III
19 NC_000016.10 16 27165785 - LINC02129 NNNTCCAGTGATCAGAGCTG NGG 2 0.4132 Tier III
20 NC_000010.11 10 5966051 - LOC107984200 NNNTCAAGGGATCTGTGCTG NGG 2 0.3302 Tier III
21 NC_000003.12 3 8001404 - LOC101927394 NNNAAAAGTGATCAGTGCTG NGG 2 0.2721 Tier III
22 NC_000018.10 18 60196966 + LOC342784 NNNCCAAGTGATGAGTGCTG NGG 2 0.0882 Tier III
23 NC_000001.11 1 63164202 + LINC00466 NNNTCAGGTGATCAGTGCTG NGA 2 0.049 Tier III
24 NC_000010.11 10 38674744 + SLC9B1P3 NNNTCAAGTGAACAGTGCTG NGT 2 0.0129 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 46805029 + Gtf2h1 NNNCCAAGTGATCAGTGCTG NGG 1 0.6471 Tier I
2 NC_000070.6 4 64008833 + Tnc NNNCCAGGTGATCAGTGCTG NGG 2 0.4567 Tier I
3 NC_000078.6 12 24581622 - Grhl1 NNNTGAACTGATCAGTGCTG NGG 2 0.3692 Tier I
4 NC_000081.6 15 92117422 + Cntn1 NNNTCAAATGATCAATGCTG NGG 2 0.9412 Tier II
5 NC_000086.7 X 49237212 - Enox2 NNNTCAAATGATCAGTGATG NGG 2 0.5385 Tier II
6 NC_000079.6 13 108374683 - Depdc1b NNNGTAAGTGATCAGTGCTG NGG 2 0.3977 Tier II
7 NC_000077.6 11 35901745 + Wwc1 NNNTCAAGTGATCAGAGCTC NGG 2 0.3896 Tier II
8 NC_000072.6 6 54448103 + Wipf3 NNNTCCAGGGATCAGTGCTG NGG 2 0.2814 Tier II
9 NC_000071.6 5 98764323 - Cfap299 NNNTCAAGTGCTCAGTGTTG NGG 2 0.2571 Tier II
10 NC_000080.6 14 117882892 - Gpc6 NNNTCAAGTGATCAGAGCTG NAG 2 0.2357 Tier II
11 NC_000075.6 9 28183945 + Opcml NNNTCATGTGATCAGTGATG NGG 2 0.2356 Tier II
12 NC_000080.6 14 47160894 - Gch1 NNNTGAAGTGATCAGTGCGG NGG 2 0.15 Tier II
13 NC_000071.6 5 103725511 - Aff1 NNNTCAAGAGATCAGTGCTG NGC 2 0.0206 Tier II
14 NC_000067.6 1 38315818 - Aff3 NNNTCAAGGGATCAGTGCTG NGC 2 0.0138 Tier II
15 NC_000073.6 7 120588039 + Gm4105 NNNTCAGGTGATCAGTGCTG NGG 1 0.7059 Tier III
16 NC_000073.6 7 120588039 + Gm46020 NNNTCAGGTGATCAGTGCTG NGG 1 0.7059 Tier III
17 NC_000070.6 4 64008833 + 8030451A03Rik NNNCCAGGTGATCAGTGCTG NGG 2 0.4567 Tier III
18 NC_000068.7 2 14538943 - Gm13266 NNNTTAACTGATCAGTGCTG NGG 2 0.3916 Tier III
19 NC_000072.6 6 32641366 - Gm32007 NNNTTAAGTGATAAGTGCTG NGG 2 0.2448 Tier III
20 NC_000079.6 13 100274819 + Naip3-ps1 NNNTCAAGTGGTCAGTGCCG NGG 2 0.1793 Tier III
21 NC_000079.6 13 100353996 + Naip3 NNNTCAAGTGGTCAGTGCCG NGG 2 0.1793 Tier III
Download CSV

Other clones with same target sequence:

(none)