Construct: sgRNA BRDN0001146266

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCATAATTAACACACATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRAF (673)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76480

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 140801438 + BRAF NNNTAATTAACACACATCAG NGG 0 1.0 Tier I
2 NC_000019.10 19 2693488 + GNG7 NNNTAATTAACATACATCAA NGG 2 0.6563 Tier II
3 NC_000007.14 7 133445330 + EXOC4 NNNTAGTTAACACACATCTG NGG 2 0.3846 Tier II
4 NC_000020.11 20 43060471 + PTPRT NNNCAAGTAACACACATCAG NGG 2 0.3806 Tier II
5 NC_000011.10 11 125481668 + FEZ1 NNNTCATTAACACACATCTG NGG 2 0.2692 Tier II
6 NC_000005.10 5 145256611 - PRELID2 NNNTTATGAACACACATCAG NGG 2 0.2667 Tier II
7 NC_000004.12 4 16534976 + LDB2 NNNTAATTATGACACATCAG NGG 2 0.2206 Tier II
8 NC_000002.12 2 148161176 + MBD5 NNNTAATAAACCCACATCAG NGG 2 0.2105 Tier II
9 NC_000002.12 2 111238850 - LOC124907867 NNNTAATTTCCACACATCAG NGG 2 0.2 Tier II
10 NC_000005.10 5 58817054 - RAB3C NNNTGATTAACAGACATCAG NGG 2 0.0982 Tier II
11 NC_000007.14 7 32274655 - PDE1C NNNTAACTAACACACATCAG NGA 2 0.0477 Tier II
12 NC_000002.12 2 108504412 + GCC2 NNNTAATTAACACATATTAG NGG 2 0.0429 Tier II
13 NC_000020.11 20 5101742 - TMEM230 NNNTAATTAACACACAACAG NGA 2 0.037 Tier II
14 NC_000020.11 20 46971677 + EYA2 NNNTAATTAGCACAGATCAG NGG 2 0.0278 Tier II
15 NC_000002.12 2 2159464 + MYT1L NNNTAATTAACACACACCAG NTG 2 0.0275 Tier II
16 NC_000011.10 11 434852 - ANO9 NNNTAATTAACAAATATCAG NGG 2 0.0256 Tier II
17 NC_000007.14 7 96220099 - SLC25A13 NNNTAATTAACACACAGCAG NTG 2 0.0046 Tier II
18 NC_000004.12 4 140044910 + MAML3 NNNAAATTAACACACTTCAG NGG 2 0.0 Tier II
19 NC_000013.11 13 73739936 - KLF12 NNNTAATTAACACACCTCAG NAG 2 0.0 Tier II
20 NC_000007.14 7 41720076 + INHBA-AS1 NNNAAATCAACACACATCAG NGG 2 0.4762 Tier III
21 NC_000023.11 X 75583639 - BRAFP1 NNNTAATTAACAAACATCAG NGG 1 0.3846 Tier III
22 NC_000023.11 X 75583639 - LOC107985664 NNNTAATTAACAAACATCAG NGG 1 0.3846 Tier III
23 NC_000004.12 4 16534976 + LOC105374505 NNNTAATTATGACACATCAG NGG 2 0.2206 Tier III
24 NC_000006.12 6 135535579 - AHI1-DT NNNTAATAAACACACATCAG NAG 2 0.2074 Tier III
25 NC_000002.12 2 111238850 - MIR4435-2HG NNNTAATTTCCACACATCAG NGG 2 0.2 Tier III
26 NC_000002.12 2 195188140 + LOC105376755 NNNAAATTACCACACATCAG NGG 2 0.1587 Tier III
27 NC_000012.12 12 22799669 + LINC02955 NNNTAATTAATACACATCAG NAG 2 0.0798 Tier III
28 NC_000011.10 11 434852 - LOC105376506 NNNTAATTAACAAATATCAG NGG 2 0.0256 Tier III
29 NC_000018.10 18 51462043 - LINC01630 NNNTAATTAACACAGAGCAG NGG 2 0.0059 Tier III
30 NC_000004.12 4 140044910 + LOC124900783 NNNAAATTAACACACTTCAG NGG 2 0.0 Tier III
31 NC_000023.11 X 36743774 + LOC105373155 NNNTAATTAACACACATCAG NCA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 39662030 + Braf NNNTAATTTACACACATCAG NGG 1 0.6 Tier I
2 NC_000075.6 9 85709021 + Ibtk NNNTAATTAAAACACATCAA NGG 2 0.7031 Tier II
3 NC_000076.6 10 31973144 + Nkain2 NNNTAATTAACACACATTAG NGG 1 0.6429 Tier II
4 NC_000069.6 3 148974623 + Adgrl2 NNNGAATTAACACACATCAG NGG 1 0.625 Tier II
5 NC_000081.6 15 21407986 - Cdh12 NNNTAATTATCACACATCAT NGG 2 0.6176 Tier II
6 NC_000072.6 6 36988596 + Dgki NNNTAGTTAACATACATCAG NGG 2 0.5 Tier II
7 NC_000078.6 12 61717785 - Lrfn5 NNNTAATTCAAACACATCAG NGG 2 0.4286 Tier II
8 NC_000083.6 17 44641784 + Runx2 NNNTACTTAAAACACATCAG NGG 2 0.3409 Tier II
9 NC_000077.6 11 82877520 + Rad51d NNNTTATTATCACACATCAG NGG 2 0.3209 Tier II
10 NC_000069.6 3 26083417 + Nlgn1 NNNTAATTATCAGACATCAG NGG 2 0.1203 Tier II
11 NC_000086.7 X 109486966 + Gm34521 NNNAAATTAACACAAATCAG NGG 2 0.1058 Tier II
12 NC_000076.6 10 6854910 + Oprm1 NNNTAATTAACACACACCAG NGA 2 0.049 Tier II
13 NC_000083.6 17 71798530 - Clip4 NNNTAATAAACACACATCAG NTG 2 0.0312 Tier II
14 NC_000073.6 7 54962967 + Luzp2 NNNTAATTAACTCACATCAG NGA 2 0.0231 Tier II
15 NC_000080.6 14 8508215 - Cfap20dc NNNTAATTAACTCACATCAG NGA 2 0.0231 Tier II
16 NC_000068.7 2 72961020 - Sp3 NNNTAATTAACAAAGATCAG NGG 2 0.0192 Tier II
17 NC_000071.6 5 49286805 - Kcnip4 NNNTCATTAACACACTTCAG NGG 2 0.0 Tier II
18 NC_000076.6 10 31973144 + Gm18189 NNNTAATTAACACACATTAG NGG 1 0.6429 Tier III
19 NC_000073.6 7 71236800 - Gm36212 NNNTGATTTACACACATCAG NGG 2 0.432 Tier III
20 NC_000073.6 7 84200776 - LOC117997403 NNNTAATTAACACACTTCAA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)