Construct: sgRNA BRDN0001146267

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTCTAGAGTATGCCCCCCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AURKB (9212)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77618

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 8206814 - AURKB NNNCTAGAGTATGCCCCCCG NGG 0 1.0 Tier I
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 61303878 + Prf1 NNNCTGGAGTATGCCCCCCA NGG 2 0.6696 Tier I
2 NC_000067.6 1 23790404 - B3gat2 NNNCTTGAGTATGCACCCCG NGG 2 0.1587 Tier II
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Other clones with same target sequence:

(none)