Construct: sgRNA BRDN0001146270
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGGGCCCAATGTATGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK3 (85443)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77312
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
36738143 |
- |
DCLK3 |
NNNGGGCCCAATGTATGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
39121321 |
+ |
TTC21A |
NNNGGGTACAATGTATGATG |
NGG |
2 |
0.5281 |
Tier II |
| 3 |
NC_000004.12 |
4 |
40756807 |
+ |
NSUN7 |
NNNGTCCCCAATGTATGATG |
NGG |
2 |
0.2045 |
Tier II |
| 4 |
NC_000001.11 |
1 |
5908090 |
+ |
NPHP4 |
NNNGGCCCCAATGTATGAAG |
NGG |
2 |
0.1948 |
Tier II |
| 5 |
NC_000017.11 |
17 |
59351734 |
- |
YPEL2 |
NNNGGGGCCAATGTATGATG |
NCG |
2 |
0.0504 |
Tier II |
| 6 |
NC_000006.12 |
6 |
165692389 |
- |
PDE10A |
NNNGGGCCCAATGTGTGATG |
NTG |
2 |
0.0253 |
Tier II |
| 7 |
NC_000010.11 |
10 |
101235594 |
- |
LBX1-AS1 |
NNNGGGCCCAACGAATGATG |
NGG |
2 |
0.5865 |
Tier III |
| 8 |
NC_000004.12 |
4 |
64938360 |
- |
LINC02232 |
NNNGGGGCCAATGTATCATG |
NGG |
2 |
0.1107 |
Tier III |
| 9 |
NC_000018.10 |
18 |
6925827 |
- |
LINC00668 |
NNNGGGCCCAATGTATGAGG |
NTG |
2 |
0.0097 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
72441581 |
+ |
Mybbp1a |
NNNAGGCCCAATGTATGATG |
NAG |
2 |
0.2333 |
Tier I |
| 2 |
NC_000081.6 |
15 |
86059235 |
+ |
Gramd4 |
NNNGGGCCCAAACTATGATG |
NGG |
2 |
0.3368 |
Tier II |
| 3 |
NC_000070.6 |
4 |
120026061 |
- |
Hivep3 |
NNNGGGCCAAATGTATTATG |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000078.6 |
12 |
81279352 |
- |
Slc8a3 |
NNNGAGCCCAATGTATGATG |
NGA |
2 |
0.0602 |
Tier II |
| 5 |
NC_000078.6 |
12 |
28946638 |
- |
Gm31508 |
NNNGGGCCCAAAGTAAGATG |
NGG |
2 |
0.7273 |
Tier III |
| 6 |
NC_000070.6 |
4 |
120026061 |
- |
Gm38632 |
NNNGGGCCAAATGTATTATG |
NGG |
2 |
0.2143 |
Tier III |
Other clones with same target sequence:
(none)
