Construct: sgRNA BRDN0001146276
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGAAATCCGGTGTAAAGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DMPK (1760)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75610
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
45777414 |
+ |
DMPK |
NNNAAATCCGGTGTAAAGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
51162074 |
- |
C13orf42 |
NNNAAATACGGTGTAAAGGT |
NGG |
2 |
0.455 |
Tier II |
| 3 |
NC_000003.12 |
3 |
16174861 |
+ |
GALNT15 |
NNNAAAACCGGGGTAAAGGG |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000002.12 |
2 |
77011806 |
- |
LRRTM4 |
NNNAAATCCTGTGTAAAGGG |
NGA |
2 |
0.0564 |
Tier II |
| 5 |
NC_000002.12 |
2 |
77013757 |
+ |
LRRTM4 |
NNNAAATCCTGTGTAAAGGG |
NGA |
2 |
0.0564 |
Tier II |
| 6 |
NC_000013.11 |
13 |
51162074 |
- |
RPL5P31 |
NNNAAATACGGTGTAAAGGT |
NGG |
2 |
0.455 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
19088178 |
- |
Dmpk |
NNNAAGTCTGGTGTAAAGGG |
NGG |
2 |
0.625 |
Tier I |
| 2 |
NC_000086.7 |
X |
9304701 |
+ |
Xk |
NNNAAATACAGTGTAAAGGG |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000072.6 |
6 |
11910136 |
+ |
Phf14 |
NNNAAAGCCAGTGTAAAGGG |
NGG |
2 |
0.549 |
Tier II |
| 4 |
NC_000085.6 |
19 |
43482411 |
- |
Cnnm1 |
NNNAAATCCTGTTTAAAGGG |
NGG |
2 |
0.2438 |
Tier II |
Other clones with same target sequence:
(none)
