Construct: sgRNA BRDN0001146280
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGTAGCGGAGCCCACGATG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- EPHB3 (2049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000003.12 | 3 | 184577938 | - | EPHB3 | NNNTAGCGGAGCCCACGATG | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000012.12 | 12 | 62807739 | - | PPM1H | NNNTAGCAGAGCCCACAATG | NGG | 2 | 0.9333 | Tier II |
| 3 | NC_000014.9 | 14 | 91320533 | + | CCDC88C | NNNTAGCGGAGCCCACGAAG | NGG | 1 | 0.2857 | Tier II |
| 4 | NC_000002.12 | 2 | 203328742 | + | ABI2 | NNNCAGCGGAGCCCCCGATG | NGG | 2 | 0.1765 | Tier II |
| 5 | NC_000004.12 | 4 | 3412307 | + | RGS12 | NNNTAGCGGGGCCCACGCTG | NGG | 2 | 0.1058 | Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
No results found.