Construct: sgRNA BRDN0001146281

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTATAAAGTTCTGGTTTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK3 (23678)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75541

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 66798561 + SGK3 NNNTAAAGTTCTGGTTTCAG NGG 0 1.0 Tier I
2 NC_000008.11 8 66798561 + C8orf44-SGK3 NNNTAAAGTTCTGGTTTCAG NGG 0 1.0 Tier I
3 NC_000004.12 4 139530724 + SETD7 NNNTAAATTTCTGGTTTCAG NGG 1 0.625 Tier II
4 NC_000021.9 21 21237798 + NCAM2 NNNTATAGTTATGGTTTCAG NGG 2 0.5357 Tier II
5 NC_000015.10 15 52008361 + MAPK6 NNNTGAAGTTCTGGTTCCAG NGG 2 0.5082 Tier II
6 NC_000016.10 16 46685624 + VPS35 NNNTAAAATGCTGGTTTCAG NGG 2 0.5 Tier II
7 NC_000015.10 15 72707788 + BBS4 NNNTAAACTTCTGATTTCAG NGG 2 0.4615 Tier II
8 NC_000001.11 1 213211277 - RPS6KC1 NNNTAAAGTTCTGGTTTTAT NGG 2 0.45 Tier II
9 NC_000001.11 1 213720972 + RPS6KC1 NNNTAACATTCTGGTTTCAG NGG 2 0.4375 Tier II
10 NC_000016.10 16 69011075 + TANGO6 NNNTAAAGTTCTGATTACAG NGG 2 0.4 Tier II
11 NC_000011.10 11 79387318 + TENM4 NNNTAAAGTTCTGGTTACAT NGG 2 0.3733 Tier II
12 NC_000004.12 4 159308584 + RAPGEF2 NNNTACAGTTCTGATTTCAG NGG 2 0.3409 Tier II
13 NC_000002.12 2 167722596 - B3GALT1 NNNTAAAATTCTGGCTTCAG NGG 2 0.2727 Tier II
14 NC_000007.14 7 147545272 - CNTNAP2 NNNTAAAGTTCTGGTTTCAG NAG 1 0.2593 Tier II
15 NC_000013.11 13 37861472 + TRPC4 NNNTTAAGTTCTGGTTTTAG NGG 2 0.2338 Tier II
16 NC_000019.10 19 56292657 - ZSCAN5A NNNTAAAGTTCTGGAGTCAG NGG 2 0.2004 Tier II
17 NC_000019.10 19 56292657 - EDDM13 NNNTAAAGTTCTGGAGTCAG NGG 2 0.2004 Tier II
18 NC_000019.10 19 56292657 - LOC124900420 NNNTAAAGTTCTGGAGTCAG NGG 2 0.2004 Tier II
19 NC_000002.12 2 208405061 + PTH2R NNNTAAAGTTGTGATTTCAG NGG 2 0.1875 Tier II
20 NC_000006.12 6 2007209 - GMDS NNNTAAAGTTGTGGTTTCAT NGG 2 0.175 Tier II
21 NC_000007.14 7 92767800 + CDK6 NNNTAAAGTTGTGGTTTCTG NGG 2 0.1346 Tier II
22 NC_000006.12 6 44117036 - MRPL14 NNNTAAAGTGCTGGTTTCAG NAG 2 0.1296 Tier II
23 NC_000003.12 3 105864728 - CBLB NNNTAATGTTCTGTTTTCAG NGG 2 0.1167 Tier II
24 NC_000005.10 5 169291352 - SLIT3 NNNTAAATTTCTGGTTTGAG NGG 2 0.0833 Tier II
25 NC_000003.12 3 146093223 - PLOD2 NNNTAAAGTTCTTGTTTCAG NAG 2 0.0778 Tier II
26 NC_000004.12 4 2653108 + FAM193A NNNTAAAGTTCTAGTTTCAG NGA 2 0.0641 Tier II
27 NC_000009.12 9 21332083 - KLHL9 NNNTAAAGTTCAGGTTTCAG NGA 2 0.0556 Tier II
28 NC_000015.10 15 42388861 + CAPN3 NNNTAAAGTTCTGGTGGCAG NGG 2 0.0407 Tier II
29 NC_000010.11 10 82572757 - NRG3 NNNTAAAGTACTGGTTTCAG NTG 2 0.0334 Tier II
30 NC_000016.10 16 12285334 - SNX29 NNNTAAAGTTCTGTTTGCAG NGG 2 0.0314 Tier II
31 NC_000017.11 17 8461544 - NDEL1 NNNTAAAGTTCTGATTTCAG NTG 2 0.0292 Tier II
32 NC_000007.14 7 27568325 + HIBADH NNNTAAAATTCTGGTTTCAG NGC 2 0.0222 Tier II
33 NC_000001.11 1 196402041 - KCNT2 NNNTAAAGTTCTGGTTTCAT NGT 2 0.0113 Tier II
34 NC_000009.12 9 83328351 - FRMD3 NNNTAAAGTTCTGGCTTCAG NTG 2 0.0106 Tier II
35 NC_000014.9 14 99203423 - BCL11B NNNTAAAGTTCTGGATTCAG NGT 2 0.0093 Tier II
36 NC_000012.12 12 97273615 - LINC02409 NNNTAAAGTTCTGGTATCAA NGG 2 0.8523 Tier III
37 NC_000021.9 21 32912836 + LOC105377136 NNNTAAATTTCTGGTATCAG NGG 2 0.5682 Tier III
38 NC_000008.11 8 128507150 + LINC00824 NNNTAGAGTGCTGGTTTCAG NGG 2 0.3571 Tier III
39 NC_000002.12 2 208405061 + LOC105373855 NNNTAAAGTTGTGATTTCAG NGG 2 0.1875 Tier III
40 NC_000012.12 12 28127414 - LOC105369710 NNNTAAAGTTCTGTTTTTAG NGG 2 0.1714 Tier III
41 NC_000012.12 12 28127414 - LOC107984462 NNNTAAAGTTCTGTTTTTAG NGG 2 0.1714 Tier III
42 NC_000006.12 6 10448211 - MIR5689HG NNNTAATGTTTTGGTTTCAG NGG 2 0.1346 Tier III
43 NC_000009.12 9 21332083 - LOC107987053 NNNTAAAGTTCAGGTTTCAG NGA 2 0.0556 Tier III
44 NC_000021.9 21 22027713 - LINC01687 NNNTAAAGTTCTGGGTTTAG NGG 2 0.0321 Tier III
45 NC_000006.12 6 74493039 + LOC101928516 NNNTAAATTTCTGGTTTCAG NTG 2 0.0244 Tier III
46 NC_000014.9 14 99203423 - LOC124903412 NNNTAAAGTTCTGGATTCAG NGT 2 0.0093 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 9868402 + Sgk3 NNNTAAAGTTCTGGTCTCTG NGG 2 0.359 Tier I
2 NC_000068.7 2 33065165 + Garnl3 NNNTATAATTCTGGTTTCAG NGG 2 0.7143 Tier II
3 NC_000077.6 11 87669648 - Rnf43 NNNTATAATTCTGGTTTCAG NGG 2 0.7143 Tier II
4 NC_000077.6 11 104894604 + Gm11639 NNNTAAAGTTCTGGTTCCAG NGG 1 0.7059 Tier II
5 NC_000071.6 5 125781138 - Tmem132b NNNTCAAGCTCTGGTTTCAG NGG 2 0.4615 Tier II
6 NC_000071.6 5 144315247 - Baiap2l1 NNNTAATGATCTGGTTTCAG NGG 2 0.4062 Tier II
7 NC_000073.6 7 66939418 + Adamts17 NNNTAAATGTCTGGTTTCAG NGG 2 0.3869 Tier II
8 NC_000084.6 18 78489956 - Slc14a2 NNNTAAAGTTCTTGTTTCAG NGG 1 0.3 Tier II
9 NC_000081.6 15 58388137 - Anxa13 NNNTAAAGTACTGTTTTCAG NGG 2 0.2286 Tier II
10 NC_000080.6 14 14860173 - Nek10 NNNCAAAGTTCTGGCTTCAG NGG 2 0.1765 Tier II
11 NC_000076.6 10 40589606 - Slc22a16 NNNTACAGTTTTGGTTTCAG NGG 2 0.1399 Tier II
12 NC_000083.6 17 63302457 + Fbxl17 NNNTAAAGTTGTGGTTACAG NGG 2 0.1333 Tier II
13 NC_000072.6 6 55071937 - Gars NNNTGAAGTTCTGGTTTCAG NTG 2 0.0281 Tier II
14 NC_000080.6 14 29102638 - Cacna2d3 NNNTTAAGTTCTGGTTTCAG NGA 2 0.0253 Tier II
15 NC_000075.6 9 75044013 - Arpp19 NNNTAAAGTTCTGGATTCAG NTG 2 0.0226 Tier II
16 NC_000075.6 9 58671147 - Rec114 NNNTAAAGTTCTGATTTCAG NGT 2 0.0121 Tier II
17 NC_000071.6 5 99379835 - A930011G23Rik NNNCAAGGTTCTGGTTTCAG NGG 2 0.4567 Tier III
18 NC_000071.6 5 99379835 - Gm35314 NNNCAAGGTTCTGGTTTCAG NGG 2 0.4567 Tier III
19 NC_000079.6 13 26306989 - 1700092E19Rik NNNTACAGCTCTGGTTTCAG NGG 2 0.4196 Tier III
20 NC_000078.6 12 113290081 - Igh NNNTAAAGTTCTAGTGTCAG NGG 2 0.3195 Tier III
21 NC_000078.6 12 113309087 - Igh NNNTAAAGTTCTAGTGTCAG NGG 2 0.3195 Tier III
22 NC_000069.6 3 52663444 + Gm30235 NNNTAAGGTTCTGTTTTCAG NGG 2 0.1882 Tier III
23 NC_000075.6 9 75044013 - Gm51714 NNNTAAAGTTCTGGATTCAG NTG 2 0.0226 Tier III
24 NC_000078.6 12 117499459 + Gm31482 NNNTAAAGTTCTGGGTTCAG NTG 2 0.0019 Tier III
25 NC_000067.6 1 180833866 + G370120E05Rik NNNTAAAGTTCTGGGTTCAG NGC 2 0.0011 Tier III
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Other clones with same target sequence:

(none)