Construct: sgRNA BRDN0001146284

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGCATGTATTTCACCAGGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME1 (4830)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75539

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 51160044 - NME1 NNNCATGTATTTCACCAGGC NGG 0 1.0 Tier I
2 NC_000017.11 17 51160044 - NME1-NME2 NNNCATGTATTTCACCAGGC NGG 0 1.0 Tier I
3 NC_000002.12 2 65016520 - SLC1A4 NNNGATGTATTTCCCCAGGC NGG 2 0.1071 Tier I
4 NC_000018.10 18 9838171 - RAB31 NNNCATGCATTCCACCAGGC NGG 2 0.9474 Tier II
5 NC_000003.12 3 197266474 - DLG1 NNNCATGTATACCACCAGGC NGG 2 0.7105 Tier II
6 NC_000005.10 5 151050886 + TNIP1 NNNGATGTATTTCACCAGGC NGG 1 0.5 Tier II
7 NC_000009.12 9 18601164 - ADAMTSL1 NNNTATGTATTTCACCAGGA NGG 2 0.4 Tier II
8 NC_000021.9 21 32428366 + EVA1C NNNCATGGATTGCACCAGGC NGG 2 0.3667 Tier II
9 NC_000003.12 3 136191995 + MSL2 NNNCATGTAAGTCACCAGGC NGG 2 0.3429 Tier II
10 NC_000002.12 2 232138935 + DIS3L2 NNNCATTTAGTTCACCAGGC NGG 2 0.2857 Tier II
11 NC_000018.10 18 28035872 + CDH2 NNNCATGTATTTCACCAGCA NGG 2 0.2241 Tier II
12 NC_000020.11 20 45853624 + ACOT8 NNNCAGGTATTTCACTAGGC NGG 2 0.1758 Tier II
13 NC_000014.9 14 71524136 - SIPA1L1 NNNCATGTATTTCAACAGAC NGG 2 0.1587 Tier II
14 NC_000015.10 15 67085714 - SMAD3 NNNCATGGATTTCCCCAGGC NGG 2 0.1571 Tier II
15 NC_000019.10 19 16417778 - EPS15L1 NNNCAGGTATTTCCCCAGGC NGG 2 0.1224 Tier II
16 NC_000011.10 11 47804860 + NUP160 NNNCATCTATTTCACCAGGC NCG 2 0.0737 Tier II
17 NC_000016.10 16 89331254 - ANKRD11 NNNAATGTATTTCATCAGGC NGG 2 0.0561 Tier II
18 NC_000016.10 16 89331254 - LOC128462377 NNNAATGTATTTCATCAGGC NGG 2 0.0561 Tier II
19 NC_000022.11 22 37952290 - C22orf23 NNNCATGAATTTCATCAGGC NGG 2 0.0533 Tier II
20 NC_000003.12 3 128966911 + CFAP92 NNNCATGTATTTCACCAGTC NGA 2 0.0463 Tier II
21 NC_000001.11 1 47420241 - LINC01389 NNNCATGTATTTTACCAGGC NGG 1 0.7 Tier III
22 NC_000003.12 3 178851814 + KCNMB2-AS1 NNNCAAGTTTTTCACCAGGC NGG 2 0.52 Tier III
23 NC_000011.10 11 3401292 - TSSC2 NNNCATGAATTGCACCAGGC NGG 2 0.4 Tier III
24 NC_000016.10 16 86556586 + FLJ30679 NNNCCTGTCTTTCACCAGGC NGG 2 0.2857 Tier III
25 NC_000008.11 8 97624879 + RPS2P33 NNNCATGTCTTTGACCAGGC NGG 2 0.0779 Tier III
26 NC_000011.10 11 86978879 - FZD4-DT NNNTATGTATTTCAGCAGGC NGG 2 0.04 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 93963265 + Nme1 NNNCATGTATTTCACCAGGC NAG 1 0.2593 Tier I
2 NC_000072.6 6 119399281 + Adipor2 NNNCATGAATCTCACCAGGC NGG 2 0.5333 Tier II
3 NC_000081.6 15 84962056 - 5031439G07Rik NNNCATGTGTCTCACCAGGC NGG 2 0.4444 Tier II
4 NC_000069.6 3 92620186 - Lce6a NNNCATGAATTTCACCAGGA NGG 2 0.4 Tier II
5 NC_000071.6 5 52660892 + Sepsecs NNNCATGTAATTCACCAGCC NGG 2 0.3842 Tier II
6 NC_000067.6 1 88199274 + Ugt1a6a NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
7 NC_000067.6 1 88199274 + Ugt1a10 NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
8 NC_000067.6 1 88199274 + Ugt1a7c NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
9 NC_000067.6 1 88199274 + Ugt1a5 NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
10 NC_000067.6 1 88199274 + Ugt1a9 NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
11 NC_000067.6 1 88199274 + Ugt1a6b NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
12 NC_000067.6 1 88199274 + Ugt1a8 NNNCATGTGTTTCACCAGGA NGG 2 0.3333 Tier II
13 NC_000072.6 6 59390940 + Gprin3 NNNCAGGTATGTCACCAGGC NGG 2 0.2286 Tier II
14 NC_000074.6 8 36930379 + Dlc1 NNNAATGTATTTCACCAGGC NTG 2 0.0328 Tier II
15 NC_000067.6 1 62740921 + Nrp2 NNNCATATATTTCACCAGGC NGT 2 0.0161 Tier II
16 NC_000069.6 3 92620186 - Gm35639 NNNCATGAATTTCACCAGGA NGG 2 0.4 Tier III
17 NC_000068.7 2 132179656 + Gm14051 NNNCATGTACTTCACCAGAC NGG 2 0.381 Tier III
18 NC_000076.6 10 84309076 - Gm5425 NNNCATGTACTTCACCAGCC NGG 2 0.2391 Tier III
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Other clones with same target sequence:

(none)