Construct: sgRNA BRDN0001146287
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAACTATAAAAGAGTTACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK1 (53354)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76557
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
89599263 |
- |
PANK1 |
NNNACTATAAAAGAGTTACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
170837754 |
+ |
GAD1 |
NNNACTATATAAGAATTACA |
NGG |
2 |
0.8304 |
Tier II |
| 3 |
NC_000008.11 |
8 |
73026857 |
+ |
TERF1 |
NNNACTATCAAAGAATTACA |
NGG |
2 |
0.5378 |
Tier II |
| 4 |
NC_000009.12 |
9 |
124872308 |
+ |
ARPC5L |
NNNATTAGAAAAGAGTTACA |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000017.11 |
17 |
20163974 |
- |
SPECC1 |
NNNTCTATTAAAGAGTTACA |
NGG |
2 |
0.3818 |
Tier II |
| 6 |
NC_000007.14 |
7 |
6402060 |
- |
RAC1 |
NNNACAATAAAAGAGGTACA |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000015.10 |
15 |
73518858 |
+ |
REC114 |
NNNACTTTAAAAGAGCTACA |
NGG |
2 |
0.2917 |
Tier II |
| 8 |
NC_000005.10 |
5 |
120724786 |
- |
PRR16 |
NNNACAATAAATGAGTTACA |
NGG |
2 |
0.2889 |
Tier II |
| 9 |
NC_000014.9 |
14 |
101848963 |
- |
PPP2R5C |
NNNACTATAAAAGACTTACA |
NGG |
1 |
0.2727 |
Tier II |
| 10 |
NC_000011.10 |
11 |
7699092 |
+ |
OVCH2 |
NNNACTATAAAAGAGTTGCT |
NGG |
2 |
0.24 |
Tier II |
| 11 |
NC_000015.10 |
15 |
77401198 |
- |
PEAK1 |
NNNATTATAAATGAGTTACA |
NGG |
2 |
0.2121 |
Tier II |
| 12 |
NC_000007.14 |
7 |
96239864 |
- |
SLC25A13 |
NNNACTATAAAGGAGTTACA |
NAG |
2 |
0.1872 |
Tier II |
| 13 |
NC_000007.14 |
7 |
135672844 |
- |
STMP1 |
NNNACTCTAAAACAGTTACA |
NGG |
2 |
0.1842 |
Tier II |
| 14 |
NC_000023.11 |
X |
138667544 |
+ |
FGF13 |
NNNACTATAAGAGAGTTACA |
NAG |
2 |
0.1685 |
Tier II |
| 15 |
NC_000005.10 |
5 |
55893629 |
+ |
IL31RA |
NNNACTATAGAAGACTTACA |
NGG |
2 |
0.1515 |
Tier II |
| 16 |
NC_000005.10 |
5 |
6722684 |
- |
TENT4A |
NNNACTTTAAATGAGTTACA |
NGG |
2 |
0.1458 |
Tier II |
| 17 |
NC_000010.11 |
10 |
86761285 |
- |
BMPR1A |
NNNACTATAAAAGAGTAACA |
NAG |
2 |
0.1383 |
Tier II |
| 18 |
NC_000023.11 |
X |
29223209 |
+ |
IL1RAPL1 |
NNNACTTTAAAAGACTTACA |
NGG |
2 |
0.1193 |
Tier II |
| 19 |
NC_000004.12 |
4 |
40902227 |
+ |
APBB2 |
NNNACTTTAAAAGAGTTCCA |
NGG |
2 |
0.0833 |
Tier II |
| 20 |
NC_000002.12 |
2 |
222568380 |
- |
FARSB |
NNNACTATAAAAGAGTGACT |
NGG |
2 |
0.0706 |
Tier II |
| 21 |
NC_000010.11 |
10 |
72814230 |
- |
MCU |
NNNACTATAAAAGACTTCCA |
NGG |
2 |
0.0519 |
Tier II |
| 22 |
NC_000023.11 |
X |
112420396 |
+ |
RTL4 |
NNNACTATAAAAGAGTTAAA |
NGA |
2 |
0.0298 |
Tier II |
| 23 |
NC_000001.11 |
1 |
54412686 |
+ |
SSBP3 |
NNNGCTATAAAAGAGTTACA |
NTG |
2 |
0.0244 |
Tier II |
| 24 |
NC_000010.11 |
10 |
13327538 |
- |
SEPHS1 |
NNNACTATAAAAGAATTACA |
NGC |
2 |
0.0209 |
Tier II |
| 25 |
NC_000005.10 |
5 |
137896309 |
- |
PKD2L2 |
NNNACTATAAAAGAGTTAAA |
NGT |
2 |
0.0069 |
Tier II |
| 26 |
NC_000010.11 |
10 |
49147587 |
- |
FAM170B-AS1 |
NNNAGTATAAAAGAGTCACA |
NGG |
2 |
0.4235 |
Tier III |
| 27 |
NC_000007.14 |
7 |
159235284 |
+ |
PIP5K1P2 |
NNNAATATAAAAGTGTTACA |
NGG |
2 |
0.3048 |
Tier III |
| 28 |
NC_000011.10 |
11 |
7699092 |
+ |
LOC105376533 |
NNNACTATAAAAGAGTTGCT |
NGG |
2 |
0.24 |
Tier III |
| 29 |
NC_000004.12 |
4 |
136880889 |
+ |
LINC02511 |
NNNACTATAAAAGAGTAACA |
NGC |
2 |
0.0119 |
Tier III |
| 30 |
NC_000005.10 |
5 |
4758444 |
- |
LOC107986400 |
NNNACTATAAAAGAGTTATA |
NGT |
2 |
0.0074 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
69487920 |
+ |
Lrp2 |
NNNACTATGAAAGAGTTATA |
NGG |
2 |
0.3077 |
Tier I |
| 2 |
NC_000077.6 |
11 |
35777810 |
+ |
Pank3 |
NNNACTATAAAAGAGTGACT |
NGG |
2 |
0.0706 |
Tier I |
| 3 |
NC_000072.6 |
6 |
36870732 |
- |
Dgki |
NNNACTATAAAAGAATTACA |
NGG |
1 |
0.9412 |
Tier II |
| 4 |
NC_000072.6 |
6 |
77823806 |
+ |
Ctnna2 |
NNNACTATAAAAAAATTACA |
NGG |
2 |
0.8688 |
Tier II |
| 5 |
NC_000081.6 |
15 |
5193071 |
- |
Ttc33 |
NNNATTATAAAAGAATTACA |
NGG |
2 |
0.5989 |
Tier II |
| 6 |
NC_000072.6 |
6 |
33059099 |
- |
Chchd3 |
NNNACTATGTAAGAGTTACA |
NGG |
2 |
0.5882 |
Tier II |
| 7 |
NC_000077.6 |
11 |
50780156 |
- |
Adamts2 |
NNNACTAGAAAAGAGTTACT |
NGG |
2 |
0.44 |
Tier II |
| 8 |
NC_000072.6 |
6 |
77559600 |
+ |
Ctnna2 |
NNNACTTTAAAAGAATTACA |
NGG |
2 |
0.4118 |
Tier II |
| 9 |
NC_000084.6 |
18 |
23639049 |
+ |
Dtna |
NNNACTTTTAAAGAGTTACA |
NGG |
2 |
0.2625 |
Tier II |
| 10 |
NC_000069.6 |
3 |
116121757 |
+ |
Vcam1 |
NNNAATCTAAAAGAGTTACA |
NGG |
2 |
0.25 |
Tier II |
| 11 |
NC_000080.6 |
14 |
100184560 |
+ |
Klf12 |
NNNACTATAACAGAGTAACA |
NGG |
2 |
0.2133 |
Tier II |
| 12 |
NC_000067.6 |
1 |
183203157 |
+ |
Disp1 |
NNNAGTATAAAATAGTTACA |
NGG |
2 |
0.18 |
Tier II |
| 13 |
NC_000073.6 |
7 |
87672896 |
- |
Grm5 |
NNNACTATAAAAGAGTTACT |
NAG |
2 |
0.1556 |
Tier II |
| 14 |
NC_000078.6 |
12 |
53649969 |
+ |
Npas3 |
NNNCCTATACAAGAGTTACA |
NGG |
2 |
0.1176 |
Tier II |
| 15 |
NC_000086.7 |
X |
144368230 |
+ |
Alg13 |
NNNACTATAAAAGAGTTAAA |
NAG |
2 |
0.1111 |
Tier II |
| 16 |
NC_000071.6 |
5 |
33850634 |
+ |
Nsd2 |
NNNACTATAAAAGATTTTCA |
NGG |
2 |
0.0714 |
Tier II |
| 17 |
NC_000078.6 |
12 |
79073202 |
+ |
Plekhh1 |
NNNACTATAAAAGAGTGACT |
NGG |
2 |
0.0706 |
Tier II |
| 18 |
NC_000070.6 |
4 |
20583883 |
- |
Nkain3 |
NNNAATATAAAAGAGTTACA |
NGA |
2 |
0.0397 |
Tier II |
| 19 |
NC_000086.7 |
X |
79652489 |
- |
Mageb16 |
NNNATTATAAAAGAGTTACA |
NTG |
2 |
0.0248 |
Tier II |
| 20 |
NC_000084.6 |
18 |
5269765 |
+ |
Zfp438 |
NNNACTATAAAACAGTTACA |
NTG |
2 |
0.0164 |
Tier II |
| 21 |
NC_000077.6 |
11 |
85648412 |
- |
Bcas3 |
NNNACTATAAAATAGTTACA |
NTG |
2 |
0.0117 |
Tier II |
| 22 |
NC_000073.6 |
7 |
94751628 |
- |
Gm32647 |
NNNATTATAAAAGAATTACA |
NGG |
2 |
0.5989 |
Tier III |
Other clones with same target sequence:
(none)
