Construct: sgRNA BRDN0001146294
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGGTTGGATGATGTCGGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KSR1 (8844)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77859
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
27597356 |
- |
KSR1 |
NNNGTTGGATGATGTCGGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
78739725 |
+ |
TENM4 |
NNNGCTGGATGAAGTCGGGA |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000018.10 |
18 |
34837716 |
+ |
DTNA |
NNNGTTGGAGGATGTAGGGA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000002.12 |
2 |
237517596 |
+ |
MLPH |
NNNGATGGATGAAGTCGGGA |
NGG |
2 |
0.3462 |
Tier II |
| 5 |
NC_000001.11 |
1 |
245528808 |
- |
KIF26B |
NNNGTTGGATGACCTCGGGA |
NGG |
2 |
0.3383 |
Tier II |
| 6 |
NC_000013.11 |
13 |
29360910 |
- |
MTUS2 |
NNNTTTGGATGATGTCTGGA |
NGG |
2 |
0.0909 |
Tier II |
| 7 |
NC_000010.11 |
10 |
27869908 |
+ |
ODAD2 |
NNNGTTTGATGATGTGGGGA |
NGG |
2 |
0.0879 |
Tier II |
| 8 |
NC_000011.10 |
11 |
69040214 |
- |
LOC124902701 |
NNNGTAGGATGATGTCAGGA |
NGG |
2 |
0.8089 |
Tier III |
| 9 |
NC_000011.10 |
11 |
69040214 |
- |
LOC124902702 |
NNNGTAGGATGATGTCAGGA |
NGG |
2 |
0.8089 |
Tier III |
| 10 |
NC_000018.10 |
18 |
48901346 |
+ |
LOC124904355 |
NNNGATGGATGATGTGGGGA |
NGG |
2 |
0.0769 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
165898921 |
- |
Zmynd8 |
NNNGGTGGAAGATGTCGGGA |
NGG |
2 |
0.5486 |
Tier I |
| 2 |
NC_000082.6 |
16 |
63595593 |
+ |
Epha3 |
NNNGTCGGATGATGTTGGGA |
NGG |
2 |
0.2797 |
Tier I |
| 3 |
NC_000074.6 |
8 |
104518506 |
- |
Nae1 |
NNNGTTGGATGCTGTAGGGA |
NGG |
2 |
0.2632 |
Tier II |
| 4 |
NC_000074.6 |
8 |
104518638 |
- |
Nae1 |
NNNGTTGGATGCTGTAGGGA |
NGG |
2 |
0.2632 |
Tier II |
| 5 |
NC_000073.6 |
7 |
111700940 |
+ |
Galnt18 |
NNNGTTGGGTGATGTGGGGA |
NGG |
2 |
0.1026 |
Tier II |
| 6 |
NC_000074.6 |
8 |
54828132 |
- |
Gpm6a |
NNNGTTGGATGATGAGGGGA |
NGG |
2 |
0.0891 |
Tier II |
| 7 |
NC_000084.6 |
18 |
4397931 |
+ |
Mtpap |
NNNGTTGGATGATTTCGGGA |
NAG |
2 |
0.0691 |
Tier II |
| 8 |
NC_000067.6 |
1 |
38425333 |
- |
Aff3 |
NNNGTTGGATGCTGTCTGGA |
NGG |
2 |
0.0658 |
Tier II |
| 9 |
NC_000070.6 |
4 |
137140456 |
+ |
Gm31762 |
NNNGTTGGATTATATCGGGA |
NGG |
2 |
0.2885 |
Tier III |
| 10 |
NC_000077.6 |
11 |
56409597 |
- |
Gm32500 |
NNNGTTGGAGGATGTCTGGA |
NGG |
2 |
0.125 |
Tier III |
Other clones with same target sequence:
(none)
