Construct: sgRNA BRDN0001146295
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGTGATGCCAAAGTACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTRK2 (4915)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76469
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
84867392 |
- |
NTRK2 |
NNNGTGATGCCAAAGTACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
170280326 |
+ |
PRKCI |
NNNGTTATGCCAAAGTACTG |
NTG |
2 |
0.026 |
Tier I |
| 3 |
NC_000009.12 |
9 |
95104440 |
+ |
FANCC |
NNNGTGATGACAAAGCACTG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000009.12 |
9 |
95104440 |
+ |
AOPEP |
NNNGTGATGACAAAGCACTG |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000017.11 |
17 |
39981293 |
- |
PSMD3 |
NNNGTGATCCCAAAGAACTG |
NGG |
2 |
0.4895 |
Tier II |
| 6 |
NC_000013.11 |
13 |
26645275 |
+ |
WASF3 |
NNNGTGATGCCAAAGCAATG |
NGG |
2 |
0.359 |
Tier II |
| 7 |
NC_000012.12 |
12 |
129495919 |
+ |
TMEM132D |
NNNGTGATTCCAAAGTAATG |
NGG |
2 |
0.2872 |
Tier II |
| 8 |
NC_000001.11 |
1 |
245356447 |
+ |
KIF26B |
NNNGTGATCCCAAGGTACTG |
NGG |
2 |
0.2513 |
Tier II |
| 9 |
NC_000007.14 |
7 |
33500890 |
- |
BBS9 |
NNNTTGATGCCAAAGTAATG |
NGG |
2 |
0.1958 |
Tier II |
| 10 |
NC_000012.12 |
12 |
128651897 |
- |
TMEM132C |
NNNGTGAAGCCAAAGTTCTG |
NGG |
2 |
0.1067 |
Tier II |
| 11 |
NC_000015.10 |
15 |
70710026 |
- |
UACA |
NNNGTGATCCCAAAGTGCTG |
NGG |
2 |
0.095 |
Tier II |
| 12 |
NC_000012.12 |
12 |
1968117 |
- |
DCP1B |
NNNGTGATCCCAAAGTACTG |
NGA |
2 |
0.0374 |
Tier II |
| 13 |
NC_000021.9 |
21 |
6115121 |
+ |
LOC102724428 |
NNNGTGATCCCAAAGTACTG |
NGA |
2 |
0.0374 |
Tier II |
| 14 |
NC_000021.9 |
21 |
43423141 |
- |
SIK1 |
NNNGTGATCCCAAAGTACTG |
NGA |
2 |
0.0374 |
Tier II |
| 15 |
NC_000022.11 |
22 |
42342381 |
+ |
TCF20 |
NNNGTGATGCCACAGTCCTG |
NGG |
2 |
0.0372 |
Tier II |
| 16 |
NC_000009.12 |
9 |
10389471 |
- |
PTPRD |
NNNGTGATGCCAAATTACTG |
NGT |
2 |
0.0023 |
Tier II |
| 17 |
NC_000009.12 |
9 |
99704132 |
- |
LOC101928438 |
NNNGTGCTGCCAGAGTACTG |
NGG |
2 |
0.2853 |
Tier III |
| 18 |
NC_000009.12 |
9 |
99704132 |
- |
LOC124902234 |
NNNGTGCTGCCAGAGTACTG |
NGG |
2 |
0.2853 |
Tier III |
| 19 |
NC_000008.11 |
8 |
12155603 |
+ |
DEFB109D |
NNNGTGATCCCAAAGTACTG |
NGA |
2 |
0.0374 |
Tier III |
| 20 |
NC_000008.11 |
8 |
12397937 |
+ |
DEFB109A |
NNNGTGATCCCAAAGTACTG |
NGA |
2 |
0.0374 |
Tier III |
| 21 |
NC_000008.11 |
8 |
12397937 |
+ |
FAM66A |
NNNGTGATCCCAAAGTACTG |
NGA |
2 |
0.0374 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
58985951 |
- |
Ntrk2 |
NNNGTGATGCCAAAGTACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000067.6 |
1 |
159546178 |
+ |
Tnr |
NNNGTGAGGACAAAGTACTG |
NGG |
2 |
0.6356 |
Tier II |
| 3 |
NC_000083.6 |
17 |
24196610 |
+ |
Tbc1d24 |
NNNGTGAGGACAAAGTACTG |
NGG |
2 |
0.6356 |
Tier II |
| 4 |
NC_000086.7 |
X |
17262073 |
- |
Efhc2 |
NNNGTTATTCCAAAGTACTG |
NGG |
2 |
0.3556 |
Tier II |
| 5 |
NC_000068.7 |
2 |
29219368 |
+ |
Ntng2 |
NNNGTGATGCCAAGGTAATG |
NGG |
2 |
0.2513 |
Tier II |
| 6 |
NC_000076.6 |
10 |
75330293 |
- |
Adora2a |
NNNGTGTTGGCAAAGTACTG |
NGG |
2 |
0.1701 |
Tier II |
| 7 |
NC_000085.6 |
19 |
36574125 |
- |
Hectd2 |
NNNGTGAAGCCAAAGTGCTG |
NGG |
2 |
0.1412 |
Tier II |
| 8 |
NC_000075.6 |
9 |
21139605 |
- |
Cdc37 |
NNNGTTATGCCAAAGTCCTG |
NGG |
2 |
0.1176 |
Tier II |
| 9 |
NC_000086.7 |
X |
141222459 |
+ |
Col4a6 |
NNNGTGATGCCAATGTTCTG |
NGG |
2 |
0.0711 |
Tier II |
| 10 |
NC_000075.6 |
9 |
67404840 |
+ |
Tln2 |
NNNGTGATGCCAAACTGCTG |
NGG |
2 |
0.0481 |
Tier II |
| 11 |
NC_000086.7 |
X |
106656358 |
+ |
Gm5127 |
NNNGTGATGCCAAAGAACTG |
NGC |
2 |
0.0202 |
Tier II |
| 12 |
NC_000086.7 |
X |
17262073 |
- |
Gm35105 |
NNNGTTATTCCAAAGTACTG |
NGG |
2 |
0.3556 |
Tier III |
Other clones with same target sequence:
(none)
