Construct: sgRNA BRDN0001146300
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGGTATTAATTGACTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME7 (29922)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76497
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
169323163 |
- |
NME7 |
NNNGGTATTAATTGACTATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
74296948 |
+ |
TNNI3K |
NNNAGTATTAATTGAATATG |
NGG |
2 |
0.9 |
Tier II |
| 3 |
NC_000001.11 |
1 |
74296948 |
+ |
FPGT-TNNI3K |
NNNAGTATTAATTGAATATG |
NGG |
2 |
0.9 |
Tier II |
| 4 |
NC_000005.10 |
5 |
119106977 |
+ |
DMXL1 |
NNNGGTATTAATTGGATATG |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000004.12 |
4 |
107640189 |
- |
PAPSS1 |
NNNGGTATTAATTGACTATG |
NAG |
1 |
0.2593 |
Tier II |
| 6 |
NC_000007.14 |
7 |
151429140 |
+ |
CRYGN |
NNNGGCATTAATTGCCTATG |
NGG |
2 |
0.2479 |
Tier II |
| 7 |
NC_000001.11 |
1 |
63486439 |
- |
ITGB3BP |
NNNTGTATTAATTGACTTTG |
NGG |
2 |
0.1818 |
Tier II |
| 8 |
NC_000003.12 |
3 |
15718056 |
- |
BTD |
NNNGTTATTGATTGACTATG |
NGG |
2 |
0.1667 |
Tier II |
| 9 |
NC_000003.12 |
3 |
15718056 |
- |
ANKRD28 |
NNNGTTATTGATTGACTATG |
NGG |
2 |
0.1667 |
Tier II |
| 10 |
NC_000012.12 |
12 |
13752597 |
- |
GRIN2B |
NNNGGTATTAAATGACTCTG |
NGG |
2 |
0.1524 |
Tier II |
| 11 |
NC_000001.11 |
1 |
40164520 |
+ |
RLF |
NNNGGTATTAAGTGACTATG |
NGA |
2 |
0.0347 |
Tier II |
| 12 |
NC_000003.12 |
3 |
175141008 |
+ |
NAALADL2 |
NNNGGTTTTAATTGACTATG |
NGA |
2 |
0.0304 |
Tier II |
| 13 |
NC_000017.11 |
17 |
60476415 |
- |
APPBP2 |
NNNGGTATTAATTGACCATG |
NGT |
2 |
0.0114 |
Tier II |
| 14 |
NC_000002.12 |
2 |
194930735 |
+ |
LOC105376755 |
NNNGGTATTAATTAGCTATG |
NGG |
2 |
0.4875 |
Tier III |
| 15 |
NC_000010.11 |
10 |
80069126 |
- |
TMEM254-AS1 |
NNNGGTATTAATTGAATATC |
NGG |
2 |
0.4286 |
Tier III |
| 16 |
NC_000023.11 |
X |
113516795 |
+ |
LOC101928437 |
NNNAGTATTAATTGACTATC |
NGG |
2 |
0.3857 |
Tier III |
| 17 |
NC_000021.9 |
21 |
22396182 |
+ |
LOC107985508 |
NNNGGTATTAGTGGACTATG |
NGG |
2 |
0.1696 |
Tier III |
| 18 |
NC_000006.12 |
6 |
22429105 |
+ |
LOC105374971 |
NNNGGTATTAATTGACTATG |
NGT |
1 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
164332733 |
+ |
Nme7 |
NNNGGTGTTGATTGACTATG |
NGG |
2 |
0.3922 |
Tier I |
| 2 |
NC_000072.6 |
6 |
23497161 |
- |
Cadps2 |
NNNGCTATTAATTGATTATG |
NGG |
2 |
0.2418 |
Tier II |
| 3 |
NC_000071.6 |
5 |
3487882 |
- |
Cdk6 |
NNNGGTATTAATTAATTATG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000075.6 |
9 |
63509068 |
+ |
Iqch |
NNNGGTCTTAATTGACTATC |
NGG |
2 |
0.1875 |
Tier II |
| 5 |
NC_000068.7 |
2 |
181853269 |
+ |
Pcmtd2 |
NNNGGTTTTAATTCACTATG |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000082.6 |
16 |
50217158 |
- |
Bbx |
NNNGGTATTAATTGGCTAAG |
NGG |
2 |
0.1857 |
Tier II |
| 7 |
NC_000071.6 |
5 |
96403486 |
- |
Fras1 |
NNNGGTATTCATTGACAATG |
NGG |
2 |
0.1778 |
Tier II |
| 8 |
NC_000081.6 |
15 |
32552061 |
+ |
Sema5a |
NNNGGTATTTATTGTCTATG |
NGG |
2 |
0.1765 |
Tier II |
| 9 |
NC_000067.6 |
1 |
105672404 |
- |
Relch |
NNNGGTAGTAATTGACTCTG |
NGG |
2 |
0.1397 |
Tier II |
| 10 |
NC_000072.6 |
6 |
115391295 |
- |
Pparg |
NNNGGTATTATTTCACTATG |
NGG |
2 |
0.1319 |
Tier II |
| 11 |
NC_000069.6 |
3 |
145511355 |
- |
Col24a1 |
NNNGGTATTAGTTGTCTATG |
NGG |
2 |
0.13 |
Tier II |
| 12 |
NC_000069.6 |
3 |
143339950 |
+ |
Gm40159 |
NNNGGTATAATTTGACTATG |
NGG |
2 |
0.2857 |
Tier III |
| 13 |
NC_000075.6 |
9 |
63509068 |
+ |
Gm16759 |
NNNGGTCTTAATTGACTATC |
NGG |
2 |
0.1875 |
Tier III |
Other clones with same target sequence:
(none)
