Construct: sgRNA BRDN0001146305
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGGTACTGAACGATATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K15 (389840)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76102
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
19395152 |
+ |
MAP3K15 |
NNNGGTACTGAACGATATTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
59841220 |
- |
CBLIF |
NNNGGTACTAAAGGATATTG |
NGG |
2 |
0.1273 |
Tier I |
| 3 |
NC_000018.10 |
18 |
62355926 |
- |
TNFRSF11A |
NNNGGTACTGAAAGAAATTG |
NGG |
2 |
0.3497 |
Tier II |
| 4 |
NC_000008.11 |
8 |
55201270 |
+ |
XKR4 |
NNNGGTACTGAAAAATATTG |
NGG |
2 |
0.2885 |
Tier II |
| 5 |
NC_000009.12 |
9 |
126479518 |
+ |
MVB12B |
NNNTGTACTGAACGAGATTG |
NGG |
2 |
0.1259 |
Tier II |
| 6 |
NC_000006.12 |
6 |
157508811 |
- |
ZDHHC14 |
NNNGGTACTGAAAGATATTG |
NAG |
2 |
0.0997 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
110429506 |
+ |
Map2k6 |
NNNGGTCCTGAATGATATTG |
NGG |
2 |
0.3062 |
Tier II |
| 2 |
NC_000081.6 |
15 |
58993431 |
- |
Mtss1 |
NNNGGTACTGAACTATATGG |
NGG |
2 |
0.0667 |
Tier II |
| 3 |
NC_000081.6 |
15 |
58993431 |
- |
Gm41330 |
NNNGGTACTGAACTATATGG |
NGG |
2 |
0.0667 |
Tier III |
Other clones with same target sequence:
(none)
