Construct: sgRNA BRDN0001146307
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTCCAGCTAAAAGCCATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1B (8395)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77085
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
68919506 |
+ |
PIP5K1B |
NNNCCAGCTAAAAGCCATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
169541825 |
+ |
F5 |
NNNCCAGCCAAAATCCATAG |
NGG |
2 |
0.2462 |
Tier I |
| 3 |
NC_000001.11 |
1 |
242508794 |
+ |
PLD5 |
NNNCTATCTAAAAGCCATAG |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000003.12 |
3 |
114867779 |
+ |
ZBTB20 |
NNNCCAGCTATAAACCATAG |
NGG |
2 |
0.2308 |
Tier II |
| 5 |
NC_000006.12 |
6 |
127511147 |
+ |
MTCL3 |
NNNCCATCTACAAGCCATAG |
NGG |
2 |
0.2286 |
Tier II |
| 6 |
NC_000005.10 |
5 |
156480542 |
- |
SGCD |
NNNCCAGCTAAAAGCCATCA |
NGG |
2 |
0.194 |
Tier II |
| 7 |
NC_000007.14 |
7 |
127039376 |
+ |
GRM8 |
NNNCCATCTAAAAGCCAGAG |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000001.11 |
1 |
204829385 |
- |
NFASC |
NNNCCAGCTAACAGCCAAAG |
NGG |
2 |
0.1754 |
Tier II |
| 9 |
NC_000006.12 |
6 |
144686845 |
- |
UTRN |
NNNCCACCTAAAAGACATAG |
NGG |
2 |
0.1528 |
Tier II |
| 10 |
NC_000011.10 |
11 |
78960209 |
+ |
TENM4 |
NNNGCAGCTAAAAGGCATAG |
NGG |
2 |
0.025 |
Tier II |
| 11 |
NC_000009.12 |
9 |
135960463 |
+ |
UBAC1 |
NNNCCAGCTAAAAGGCACAG |
NGG |
2 |
0.0214 |
Tier II |
| 12 |
NC_000001.11 |
1 |
110486471 |
- |
CYMP |
NNNCCAGCAAAAAGCCATAG |
NAG |
2 |
0.2407 |
Tier III |
| 13 |
NC_000006.12 |
6 |
127511147 |
+ |
LOC107986642 |
NNNCCATCTACAAGCCATAG |
NGG |
2 |
0.2286 |
Tier III |
| 14 |
NC_000006.12 |
6 |
127511147 |
+ |
SOGA3-KIAA0408 |
NNNCCATCTACAAGCCATAG |
NGG |
2 |
0.2286 |
Tier III |
| 15 |
NC_000014.9 |
14 |
106836368 |
- |
IGH |
NNNCCAGCTAGACGCCATAG |
NGG |
2 |
0.1368 |
Tier III |
| 16 |
NC_000017.11 |
17 |
32429678 |
+ |
NUDT15P2 |
NNNCAAGCTAAAAGCCTTAG |
NGG |
2 |
0.0762 |
Tier III |
| 17 |
NC_000007.14 |
7 |
66003468 |
- |
LOC105375336 |
NNNCCAGTTAAAAGCCATAG |
NTG |
2 |
0.0341 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
24358268 |
- |
Pip5k1b |
NNNCCAGCTAAAAGCCATAA |
NGG |
1 |
0.9375 |
Tier I |
| 2 |
NC_000075.6 |
9 |
119200048 |
- |
Slc22a13 |
NNNCCTGGTAAAAGCCATAG |
NGG |
2 |
0.4592 |
Tier II |
| 3 |
NC_000086.7 |
X |
103722920 |
- |
Slc16a2 |
NNNCCAGCTGAATGCCATAG |
NGG |
2 |
0.1667 |
Tier II |
| 4 |
NC_000081.6 |
15 |
98068609 |
+ |
Senp1 |
NNNCCAGCTAAACGCCATTG |
NGG |
2 |
0.1134 |
Tier II |
| 5 |
NC_000071.6 |
5 |
23511760 |
+ |
Srpk2 |
NNNCCAGCTGAAAGCCATAG |
NGA |
2 |
0.0386 |
Tier II |
| 6 |
NC_000073.6 |
7 |
30620507 |
+ |
Cox6b1 |
NNNCCAGCTAAAGGGCATAG |
NGG |
2 |
0.0326 |
Tier II |
| 7 |
NC_000073.6 |
7 |
122673685 |
+ |
Cacng3 |
NNNCCAGCTAAAAGCCATAC |
NGA |
2 |
0.0298 |
Tier II |
| 8 |
NC_000080.6 |
14 |
93328175 |
+ |
Pcdh9 |
NNNCCAGCTAAAAGTCATCG |
NGG |
2 |
0.0138 |
Tier II |
| 9 |
NC_000080.6 |
14 |
27484942 |
+ |
Ccdc66 |
NNNCCAGCTAAAAGCCATAG |
NAA |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
