Construct: sgRNA BRDN0001146308
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGAACAGTCGGCTACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK4 (2868)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77724
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
3027997 |
+ |
GRK4 |
NNNGGAACAGTCGGCTACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
168305164 |
+ |
DDX60 |
NNNGGAACAGTAGGCTACAT |
NAG |
2 |
0.1852 |
Tier II |
| 3 |
NC_000009.12 |
9 |
28768430 |
- |
LINGO2 |
NNNGGAACAGTCTGCTACAT |
NAG |
2 |
0.0778 |
Tier II |
| 4 |
NC_000008.11 |
8 |
12973041 |
- |
TRMT9B |
NNNGGAACAGTCAGCTACAT |
NGA |
2 |
0.0641 |
Tier II |
| 5 |
NC_000007.14 |
7 |
151677717 |
- |
PRKAG2 |
NNNGGAACAGTTGGCTACAT |
NTG |
2 |
0.021 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
103790524 |
- |
Caprin1 |
NNNAGAACAGTTGGCTACAT |
NGG |
2 |
0.4846 |
Tier II |
| 2 |
NC_000078.6 |
12 |
98438180 |
- |
Kcnk10 |
NNNTGAACAGTTGGCTACAT |
NGG |
2 |
0.1958 |
Tier II |
| 3 |
NC_000072.6 |
6 |
61786916 |
+ |
Ccser1 |
NNNGGAACAGTGGGCTACAT |
NGC |
2 |
0.0099 |
Tier II |
| 4 |
NC_000070.6 |
4 |
65912135 |
+ |
Astn2 |
NNNGGAACAGTCGGGTACAC |
NGG |
2 |
0.0045 |
Tier II |
| 5 |
NC_000075.6 |
9 |
119781082 |
+ |
Scn11a |
NNNGGAACAGTCGGGTACAT |
NTG |
2 |
0.0019 |
Tier II |
Other clones with same target sequence:
(none)
