Construct: sgRNA BRDN0001146311

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AACTGGTCCATGATGCCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALK1 (2584)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76704

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 75763079 + GALK1 NNNTGGTCCATGATGCCACA NGG 0 1.0 Tier I
2 NC_000003.12 3 126136344 + ALDH1L1 NNNTGGCTCATGATGCCACA NGG 2 0.6016 Tier II
3 NC_000023.11 X 71305273 - ITGB1BP2 NNNTGCTCAATGATGCCACA NGG 2 0.5844 Tier II
4 NC_000016.10 16 75572875 + GABARAPL2 NNNTGGTACTTGATGCCACA NGG 2 0.5735 Tier II
5 NC_000017.11 17 33039236 + ASIC2 NNNTGTTCCATGGTGCCACA NGG 2 0.4348 Tier II
6 NC_000013.11 13 111181678 - ARHGEF7 NNNTGTTCCATGATGCCTCA NGG 2 0.3333 Tier II
7 NC_000007.14 7 87734365 + RUNDC3B NNNTGATCCATGCTGCCACA NGG 2 0.2105 Tier II
8 NC_000002.12 2 110113707 - MALL NNNTGGTCCAGGATCCCACA NGG 2 0.1091 Tier II
9 NC_000010.11 10 22676029 + PIP4K2A NNNTGCTCCATGATGGCACA NGG 2 0.1049 Tier II
10 NC_000009.12 9 4240142 - GLIS3 NNNTGGTCCCTGATGCCACA NAG 2 0.0864 Tier II
11 NC_000002.12 2 20561498 + HS1BP3 NNNTGGTCCACGATGCCACA NTG 2 0.026 Tier II
12 NC_000010.11 10 24494857 - KIAA1217 NNNTGGTCCATGAAGCCACA NGC 2 0.0138 Tier II
13 NC_000011.10 11 70742175 - SHANK2 NNNTGGCCCATGAGGCCACA NGG 2 0.0 Tier II
14 NC_000019.10 19 1618693 - TCF3 NNNTGGCCCATGAGGCCACA NGG 2 0.0 Tier II
15 NC_000008.11 8 57546331 + LOC105375855 NNNTAGTTCATGATGCCACA NGG 2 0.7583 Tier III
16 NC_000004.12 4 36258354 - LOC439933 NNNTGCTCAATGATGCCACA NGG 2 0.5844 Tier III
17 NC_000003.12 3 25948056 - LOC124909357 NNNTCGTCCATGATGCCAGA NGG 2 0.0982 Tier III
18 NC_000016.10 16 50863560 - LINC02128 NNNTGGTCCATGCTGCCACA NAG 2 0.0546 Tier III
19 NC_000002.12 2 108317867 - SULT1C2P2 NNNTGGTCCATGATGTCACA NGT 2 0.005 Tier III
20 NC_000001.11 1 211683600 + NEK2-DT NNNTGGTCCATGATGCCAGA NGT 2 0.002 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 116010306 + Galk1 NNNTGGTCCATGATGCCACA NGG 0 1.0 Tier I
2 NC_000070.6 4 136607428 + Tex46 NNNTGGTCCTTGATGCCACC NGG 2 0.2005 Tier I
3 NC_000075.6 9 113796328 - Clasp2 NNNTGGTCAAAGATGCCACA NGG 2 0.6429 Tier II
4 NC_000068.7 2 110704153 + Ano3 NNNTGCTCCATGATACCACA NGG 2 0.6417 Tier II
5 NC_000081.6 15 28286952 - Dnah5 NNNTGTTCCATGATACCACA NGG 2 0.6275 Tier II
6 NC_000083.6 17 30859396 + Dnah8 NNNAGATCCATGATGCCACA NGG 2 0.4762 Tier II
7 NC_000081.6 15 53653266 + Samd12 NNNTGCCCCATGATGCCACA NGG 2 0.4687 Tier II
8 NC_000086.7 X 138292275 - Il1rapl2 NNNTGCCCCATGATGCCACA NGG 2 0.4687 Tier II
9 NC_000070.6 4 107080741 - Cyb5rl NNNTGGTGCATGAAGCCACA NGG 2 0.398 Tier II
10 NC_000075.6 9 10207629 + Cntn5 NNNTGTGCCATGATGCCACA NGG 2 0.3922 Tier II
11 NC_000072.6 6 108222216 + Itpr1 NNNTGGGCCATGAAGCCACA NGG 2 0.3641 Tier II
12 NC_000070.6 4 46608299 - Tbc1d2 NNNTGGTCCTTGATGCCGCA NGG 2 0.3529 Tier II
13 NC_000070.6 4 86082381 + Adamtsl1 NNNTGGCCCATGATGCCGCA NGG 2 0.275 Tier II
14 NC_000081.6 15 57699860 - Zhx2 NNNTGGACCATGATGCCACA NAG 2 0.2269 Tier II
15 NC_000080.6 14 45306679 + Ero1a NNNTGGTCCCTGGTGCCACA NGG 2 0.2174 Tier II
16 NC_000068.7 2 140306191 - Sel1l2 NNNTGATCCATGCTGCCACA NGG 2 0.2105 Tier II
17 NC_000067.6 1 80642310 + Dock10 NNNTGGTCCCTGAAGCCACA NGG 2 0.2063 Tier II
18 NC_000075.6 9 121073126 + Ulk4 NNNTGGTCCCTGAAGCCACA NGG 2 0.2063 Tier II
19 NC_000086.7 X 157298854 - Phex NNNTGGTCTATGATGCCACC NGG 2 0.1989 Tier II
20 NC_000068.7 2 161068285 - Chd6 NNNTGGTCCATGCTACCACA NGG 2 0.1981 Tier II
21 NC_000083.6 17 66403631 + Mtcl1 NNNTGGTCCATGATACCCCA NGG 2 0.1793 Tier II
22 NC_000072.6 6 113654523 - Irak2 NNNAGGTCCCTGATGCCACA NGG 2 0.1587 Tier II
23 NC_000071.6 5 22877822 - Lhfpl3 NNNTGGTCCATGCTGCCACT NGG 2 0.1263 Tier II
24 NC_000073.6 7 76309795 + Agbl1 NNNTGGTCCATGCTGCCACT NGG 2 0.1263 Tier II
25 NC_000076.6 10 59838440 - Micu1 NNNTTGTCCATGCTGCCACA NGG 2 0.0632 Tier II
26 NC_000076.6 10 39065559 - Lama4 NNNTGGTCCATGATGCCTGA NGG 2 0.0625 Tier II
27 NC_000072.6 6 37439792 - Creb3l2 NNNTTGTCCATGATGGCACA NGG 2 0.0462 Tier II
28 NC_000083.6 17 62684969 - Efna5 NNNTGGTCCCTGATGCCACA NCG 2 0.0357 Tier II
29 NC_000073.6 7 134394456 - D7Ertd443e NNNTGGTCCATGGTGCCACA NGC 2 0.0145 Tier II
30 NC_000078.6 12 83265907 + Dpf3 NNNTGGTCCATGAGGTCACA NGG 2 0.0 Tier II
31 NC_000077.6 11 115835403 - Llgl2 NNNTGGTCCTTGAGGCCACA NGG 2 0.0 Tier II
32 NC_000070.6 4 46608299 - Gm31850 NNNTGGTCCTTGATGCCGCA NGG 2 0.3529 Tier III
33 NC_000084.6 18 68576129 - Gm30700 NNNTGGTCCAATATGCCACA NGG 2 0.2885 Tier III
34 NC_000069.6 3 27810584 - Gm32795 NNNTGATCCATGCTGCCACA NGG 2 0.2105 Tier III
35 NC_000069.6 3 27810584 - Gm42194 NNNTGATCCATGCTGCCACA NGG 2 0.2105 Tier III
36 NC_000082.6 16 23179445 + C730014E05Rik NNNTGATCCATGCTGCCACA NGG 2 0.2105 Tier III
37 NC_000081.6 15 18061846 + Gm41277 NNNTGGTCCATGCTGCCACT NGG 2 0.1263 Tier III
38 NC_000069.6 3 105133280 + Gm40118 NNNTGGTCCATGATGCCTCA NCG 2 0.0536 Tier III
39 NC_000075.6 9 43718321 + Gm30015 NNNTTGTCCATGATGCCACA NGA 2 0.0208 Tier III
Download CSV

Other clones with same target sequence:

(none)