Construct: sgRNA BRDN0001146311
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACTGGTCCATGATGCCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GALK1 (2584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76704
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000017.11 |
17 |
75763079 |
+ |
GALK1 |
NNNTGGTCCATGATGCCACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000003.12 |
3 |
126136344 |
+ |
ALDH1L1 |
NNNTGGCTCATGATGCCACA |
NGG |
2 |
0.6016 |
Tier II |
3 |
NC_000023.11 |
X |
71305273 |
- |
ITGB1BP2 |
NNNTGCTCAATGATGCCACA |
NGG |
2 |
0.5844 |
Tier II |
4 |
NC_000016.10 |
16 |
75572875 |
+ |
GABARAPL2 |
NNNTGGTACTTGATGCCACA |
NGG |
2 |
0.5735 |
Tier II |
5 |
NC_000017.11 |
17 |
33039236 |
+ |
ASIC2 |
NNNTGTTCCATGGTGCCACA |
NGG |
2 |
0.4348 |
Tier II |
6 |
NC_000013.11 |
13 |
111181678 |
- |
ARHGEF7 |
NNNTGTTCCATGATGCCTCA |
NGG |
2 |
0.3333 |
Tier II |
7 |
NC_000007.14 |
7 |
87734365 |
+ |
RUNDC3B |
NNNTGATCCATGCTGCCACA |
NGG |
2 |
0.2105 |
Tier II |
8 |
NC_000002.12 |
2 |
110113707 |
- |
MALL |
NNNTGGTCCAGGATCCCACA |
NGG |
2 |
0.1091 |
Tier II |
9 |
NC_000010.11 |
10 |
22676029 |
+ |
PIP4K2A |
NNNTGCTCCATGATGGCACA |
NGG |
2 |
0.1049 |
Tier II |
10 |
NC_000009.12 |
9 |
4240142 |
- |
GLIS3 |
NNNTGGTCCCTGATGCCACA |
NAG |
2 |
0.0864 |
Tier II |
11 |
NC_000002.12 |
2 |
20561498 |
+ |
HS1BP3 |
NNNTGGTCCACGATGCCACA |
NTG |
2 |
0.026 |
Tier II |
12 |
NC_000010.11 |
10 |
24494857 |
- |
KIAA1217 |
NNNTGGTCCATGAAGCCACA |
NGC |
2 |
0.0138 |
Tier II |
13 |
NC_000011.10 |
11 |
70742175 |
- |
SHANK2 |
NNNTGGCCCATGAGGCCACA |
NGG |
2 |
0.0 |
Tier II |
14 |
NC_000019.10 |
19 |
1618693 |
- |
TCF3 |
NNNTGGCCCATGAGGCCACA |
NGG |
2 |
0.0 |
Tier II |
15 |
NC_000008.11 |
8 |
57546331 |
+ |
LOC105375855 |
NNNTAGTTCATGATGCCACA |
NGG |
2 |
0.7583 |
Tier III |
16 |
NC_000004.12 |
4 |
36258354 |
- |
LOC439933 |
NNNTGCTCAATGATGCCACA |
NGG |
2 |
0.5844 |
Tier III |
17 |
NC_000003.12 |
3 |
25948056 |
- |
LOC124909357 |
NNNTCGTCCATGATGCCAGA |
NGG |
2 |
0.0982 |
Tier III |
18 |
NC_000016.10 |
16 |
50863560 |
- |
LINC02128 |
NNNTGGTCCATGCTGCCACA |
NAG |
2 |
0.0546 |
Tier III |
19 |
NC_000002.12 |
2 |
108317867 |
- |
SULT1C2P2 |
NNNTGGTCCATGATGTCACA |
NGT |
2 |
0.005 |
Tier III |
20 |
NC_000001.11 |
1 |
211683600 |
+ |
NEK2-DT |
NNNTGGTCCATGATGCCAGA |
NGT |
2 |
0.002 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000077.6 |
11 |
116010306 |
+ |
Galk1 |
NNNTGGTCCATGATGCCACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000070.6 |
4 |
136607428 |
+ |
Tex46 |
NNNTGGTCCTTGATGCCACC |
NGG |
2 |
0.2005 |
Tier I |
3 |
NC_000075.6 |
9 |
113796328 |
- |
Clasp2 |
NNNTGGTCAAAGATGCCACA |
NGG |
2 |
0.6429 |
Tier II |
4 |
NC_000068.7 |
2 |
110704153 |
+ |
Ano3 |
NNNTGCTCCATGATACCACA |
NGG |
2 |
0.6417 |
Tier II |
5 |
NC_000081.6 |
15 |
28286952 |
- |
Dnah5 |
NNNTGTTCCATGATACCACA |
NGG |
2 |
0.6275 |
Tier II |
6 |
NC_000083.6 |
17 |
30859396 |
+ |
Dnah8 |
NNNAGATCCATGATGCCACA |
NGG |
2 |
0.4762 |
Tier II |
7 |
NC_000081.6 |
15 |
53653266 |
+ |
Samd12 |
NNNTGCCCCATGATGCCACA |
NGG |
2 |
0.4687 |
Tier II |
8 |
NC_000086.7 |
X |
138292275 |
- |
Il1rapl2 |
NNNTGCCCCATGATGCCACA |
NGG |
2 |
0.4687 |
Tier II |
9 |
NC_000070.6 |
4 |
107080741 |
- |
Cyb5rl |
NNNTGGTGCATGAAGCCACA |
NGG |
2 |
0.398 |
Tier II |
10 |
NC_000075.6 |
9 |
10207629 |
+ |
Cntn5 |
NNNTGTGCCATGATGCCACA |
NGG |
2 |
0.3922 |
Tier II |
11 |
NC_000072.6 |
6 |
108222216 |
+ |
Itpr1 |
NNNTGGGCCATGAAGCCACA |
NGG |
2 |
0.3641 |
Tier II |
12 |
NC_000070.6 |
4 |
46608299 |
- |
Tbc1d2 |
NNNTGGTCCTTGATGCCGCA |
NGG |
2 |
0.3529 |
Tier II |
13 |
NC_000070.6 |
4 |
86082381 |
+ |
Adamtsl1 |
NNNTGGCCCATGATGCCGCA |
NGG |
2 |
0.275 |
Tier II |
14 |
NC_000081.6 |
15 |
57699860 |
- |
Zhx2 |
NNNTGGACCATGATGCCACA |
NAG |
2 |
0.2269 |
Tier II |
15 |
NC_000080.6 |
14 |
45306679 |
+ |
Ero1a |
NNNTGGTCCCTGGTGCCACA |
NGG |
2 |
0.2174 |
Tier II |
16 |
NC_000068.7 |
2 |
140306191 |
- |
Sel1l2 |
NNNTGATCCATGCTGCCACA |
NGG |
2 |
0.2105 |
Tier II |
17 |
NC_000067.6 |
1 |
80642310 |
+ |
Dock10 |
NNNTGGTCCCTGAAGCCACA |
NGG |
2 |
0.2063 |
Tier II |
18 |
NC_000075.6 |
9 |
121073126 |
+ |
Ulk4 |
NNNTGGTCCCTGAAGCCACA |
NGG |
2 |
0.2063 |
Tier II |
19 |
NC_000086.7 |
X |
157298854 |
- |
Phex |
NNNTGGTCTATGATGCCACC |
NGG |
2 |
0.1989 |
Tier II |
20 |
NC_000068.7 |
2 |
161068285 |
- |
Chd6 |
NNNTGGTCCATGCTACCACA |
NGG |
2 |
0.1981 |
Tier II |
21 |
NC_000083.6 |
17 |
66403631 |
+ |
Mtcl1 |
NNNTGGTCCATGATACCCCA |
NGG |
2 |
0.1793 |
Tier II |
22 |
NC_000072.6 |
6 |
113654523 |
- |
Irak2 |
NNNAGGTCCCTGATGCCACA |
NGG |
2 |
0.1587 |
Tier II |
23 |
NC_000071.6 |
5 |
22877822 |
- |
Lhfpl3 |
NNNTGGTCCATGCTGCCACT |
NGG |
2 |
0.1263 |
Tier II |
24 |
NC_000073.6 |
7 |
76309795 |
+ |
Agbl1 |
NNNTGGTCCATGCTGCCACT |
NGG |
2 |
0.1263 |
Tier II |
25 |
NC_000076.6 |
10 |
59838440 |
- |
Micu1 |
NNNTTGTCCATGCTGCCACA |
NGG |
2 |
0.0632 |
Tier II |
26 |
NC_000076.6 |
10 |
39065559 |
- |
Lama4 |
NNNTGGTCCATGATGCCTGA |
NGG |
2 |
0.0625 |
Tier II |
27 |
NC_000072.6 |
6 |
37439792 |
- |
Creb3l2 |
NNNTTGTCCATGATGGCACA |
NGG |
2 |
0.0462 |
Tier II |
28 |
NC_000083.6 |
17 |
62684969 |
- |
Efna5 |
NNNTGGTCCCTGATGCCACA |
NCG |
2 |
0.0357 |
Tier II |
29 |
NC_000073.6 |
7 |
134394456 |
- |
D7Ertd443e |
NNNTGGTCCATGGTGCCACA |
NGC |
2 |
0.0145 |
Tier II |
30 |
NC_000078.6 |
12 |
83265907 |
+ |
Dpf3 |
NNNTGGTCCATGAGGTCACA |
NGG |
2 |
0.0 |
Tier II |
31 |
NC_000077.6 |
11 |
115835403 |
- |
Llgl2 |
NNNTGGTCCTTGAGGCCACA |
NGG |
2 |
0.0 |
Tier II |
32 |
NC_000070.6 |
4 |
46608299 |
- |
Gm31850 |
NNNTGGTCCTTGATGCCGCA |
NGG |
2 |
0.3529 |
Tier III |
33 |
NC_000084.6 |
18 |
68576129 |
- |
Gm30700 |
NNNTGGTCCAATATGCCACA |
NGG |
2 |
0.2885 |
Tier III |
34 |
NC_000069.6 |
3 |
27810584 |
- |
Gm32795 |
NNNTGATCCATGCTGCCACA |
NGG |
2 |
0.2105 |
Tier III |
35 |
NC_000069.6 |
3 |
27810584 |
- |
Gm42194 |
NNNTGATCCATGCTGCCACA |
NGG |
2 |
0.2105 |
Tier III |
36 |
NC_000082.6 |
16 |
23179445 |
+ |
C730014E05Rik |
NNNTGATCCATGCTGCCACA |
NGG |
2 |
0.2105 |
Tier III |
37 |
NC_000081.6 |
15 |
18061846 |
+ |
Gm41277 |
NNNTGGTCCATGCTGCCACT |
NGG |
2 |
0.1263 |
Tier III |
38 |
NC_000069.6 |
3 |
105133280 |
+ |
Gm40118 |
NNNTGGTCCATGATGCCTCA |
NCG |
2 |
0.0536 |
Tier III |
39 |
NC_000075.6 |
9 |
43718321 |
+ |
Gm30015 |
NNNTTGTCCATGATGCCACA |
NGA |
2 |
0.0208 |
Tier III |
Other clones with same target sequence:
(none)