Construct: sgRNA BRDN0001146314
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATTGTAGAGGGATCCATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ILK (3611)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75910
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
6609606 |
- |
ILK |
NNNTTGTAGAGGGATCCATA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
79432720 |
+ |
ZCCHC24 |
NNNTTGTTGAGGGAGCCATA |
NGG |
2 |
0.0214 |
Tier I |
| 3 |
NC_000011.10 |
11 |
6609606 |
- |
TAF10 |
NNNTTGTAGAGGGATCCATA |
NGG |
0 |
1.0 |
Tier II |
| 4 |
NC_000003.12 |
3 |
61972280 |
+ |
PTPRG |
NNNCTGTAGAGGGATCCATG |
NGG |
2 |
0.4948 |
Tier II |
| 5 |
NC_000023.11 |
X |
2803597 |
+ |
XG |
NNNTTGCAGAGGGAACCATA |
NGG |
2 |
0.398 |
Tier II |
| 6 |
NC_000010.11 |
10 |
13107993 |
- |
OPTN |
NNNTTGTAGAGGCATCCATG |
NGG |
2 |
0.322 |
Tier II |
| 7 |
NC_000001.11 |
1 |
61318065 |
- |
NFIA |
NNNTTGTAAAGGGATCTATA |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000001.11 |
1 |
20293509 |
- |
VWA5B1 |
NNNTTGTAGAGGGAACCATC |
NGG |
2 |
0.1316 |
Tier II |
| 9 |
NC_000011.10 |
11 |
122915044 |
+ |
JHY |
NNNTTGAAGAGGGATCCATA |
NGA |
2 |
0.0608 |
Tier II |
| 10 |
NC_000001.11 |
1 |
2101073 |
+ |
PRKCZ |
NNNTTGTAGAGGGATCCCAA |
NGG |
2 |
0.0544 |
Tier II |
| 11 |
NC_000010.11 |
10 |
98203645 |
+ |
R3HCC1L |
NNNTTGAAGAGGGATCCATA |
NGT |
2 |
0.0141 |
Tier II |
| 12 |
NC_000014.9 |
14 |
76635712 |
- |
RPSAP3 |
NNNCTGCAGAGGGATCCATA |
NGG |
2 |
0.4449 |
Tier III |
| 13 |
NC_000024.10 |
Y |
12448127 |
- |
LOC124905301 |
NNNTTGCAGAGGGAACCATA |
NGG |
2 |
0.398 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
105741674 |
- |
Ilk |
NNNTTGTAGAGAGATCCATA |
NGG |
1 |
0.9333 |
Tier I |
| 2 |
NC_000079.6 |
13 |
74019329 |
+ |
Tppp |
NNNTTGTAGAGAGATCCATA |
NGG |
1 |
0.9333 |
Tier II |
| 3 |
NC_000073.6 |
7 |
111068029 |
+ |
Eif4g2 |
NNNTTGTAGAGGAGTCCATA |
NGG |
2 |
0.4308 |
Tier II |
| 4 |
NC_000076.6 |
10 |
74200680 |
- |
Pcdh15 |
NNNTTGTAGAGTGAACCATA |
NGG |
2 |
0.2227 |
Tier II |
| 5 |
NC_000072.6 |
6 |
93750002 |
- |
Magi1 |
NNNTTGTAGATGGATCCCTA |
NGG |
2 |
0.0733 |
Tier II |
| 6 |
NC_000072.6 |
6 |
4930240 |
- |
Ppp1r9a |
NNNTTGTAGAGGGATGCACA |
NGG |
2 |
0.0424 |
Tier II |
| 7 |
NC_000080.6 |
14 |
27313648 |
+ |
Arhgef3 |
NNNTTGTAGAGGGAGCCATT |
NGG |
2 |
0.03 |
Tier II |
| 8 |
NC_000068.7 |
2 |
146340925 |
- |
Ralgapa2 |
NNNTTGTAGATGGATCGATA |
NGG |
2 |
0.0226 |
Tier II |
| 9 |
NC_000079.6 |
13 |
76980698 |
- |
Mctp1 |
NNNTTGTAGAGGGAGTCATA |
NGG |
2 |
0.0154 |
Tier II |
| 10 |
NC_000079.6 |
13 |
74019329 |
+ |
Gm6263 |
NNNTTGTAGAGAGATCCATA |
NGG |
1 |
0.9333 |
Tier III |
Other clones with same target sequence:
(none)
