Construct: sgRNA BRDN0001146317
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAGCAGGACGAGGTTCCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80213
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
110871801 |
- |
CCDC63 |
NNNTCAGGATGAGGTTCCTT |
NGG |
2 |
0.3422 |
Tier II |
| 2 |
NC_000007.14 |
7 |
4014156 |
+ |
SDK1 |
NNNGCAGGAAGAGTTTCCTT |
NGG |
2 |
0.2311 |
Tier II |
| 3 |
NC_000004.12 |
4 |
128857583 |
+ |
JADE1 |
NNNGCAGGACGAGGTTCCCA |
NGG |
2 |
0.1552 |
Tier II |
| 4 |
NC_000009.12 |
9 |
110668874 |
- |
MUSK |
NNNGCAGGACGAAGTTCCTT |
NTG |
2 |
0.036 |
Tier II |
| 5 |
NC_000001.11 |
1 |
205243073 |
+ |
TMCC2 |
NNNGCAGGACGAGGTTCCTC |
NAG |
2 |
0.0236 |
Tier II |
| 6 |
NC_000017.11 |
17 |
34885553 |
+ |
LOC105371742 |
NNNGCAGGATGAGGTTCCCT |
NGG |
2 |
0.2596 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
75537272 |
- |
Iqcm |
NNNGCAGGAAGAGGTTCCTG |
NGG |
2 |
0.1529 |
Tier II |
| 2 |
NC_000082.6 |
16 |
35403647 |
+ |
Pdia5 |
NNNGCAGGACGAGGATCCTC |
NGG |
2 |
0.0526 |
Tier II |
Other clones with same target sequence:
(none)
