Construct: sgRNA BRDN0001146318

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTGCTTTCCCATACCCGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K6 (9064)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76014

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 27358820 + MAP3K6 NNNGCTTTCCCATACCCGCG NGG 0 1.0 Tier I
2 NC_000010.11 10 97321194 - FRAT1 NNNGCTTTCCCACACCCGCG NTG 2 0.0308 Tier II
3 NC_000001.11 1 943204 - SAMD11 NNNGCTTTCCCATACCCGCC NGT 2 0.0069 Tier II
4 NC_000017.11 17 40121403 + LOC124904001 NNNTCTCTCCCATACCCGCG NGG 2 0.25 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 108780599 - Zfyve9 NNNGCTTTCCCGGACCCGCG NGG 2 0.1884 Tier II
2 NC_000082.6 16 34029671 - Kalrn NNNGCTTTCCCATCCCAGCG NGG 2 0.1 Tier II
3 NC_000077.6 11 33993709 - 4930469K13Rik NNNGCTTTCCCATCCCCGGG NGG 2 0.0268 Tier III
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Other clones with same target sequence:

(none)