Construct: sgRNA BRDN0001146321
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTCACATGTGGTGGAATGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CSNK2A1 (1457)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000020.11 | 20 | 508456 | - | CSNK2A1 | NNNCACATGTGGTGGAATGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000011.10 | 11 | 11353024 | - | CSNK2A3 | NNNCACATGTGGTGGAATGG | NGA | 1 | 0.0694 | Tier I |
3 | NC_000012.12 | 12 | 112240002 | - | HECTD4 | NNNCACATGTGGTGGGATGG | NCG | 2 | 0.0206 | Tier I |
4 | NC_000006.12 | 6 | 45385287 | - | RUNX2 | NNNCAAATGAGGTGGAATGG | NGG | 2 | 0.7959 | Tier II |
5 | NC_000018.10 | 18 | 23275919 | + | TMEM241 | NNNCACAGGTGGTAGAATGG | NGG | 2 | 0.55 | Tier II |
6 | NC_000006.12 | 6 | 2748378 | + | MYLK4 | NNNCACATGTGCTGGAATGA | NGG | 2 | 0.4963 | Tier II |
7 | NC_000009.12 | 9 | 121112633 | + | CNTRL | NNNCACATGGGATGGAATGG | NGG | 2 | 0.4667 | Tier II |
8 | NC_000001.11 | 1 | 18126388 | - | IGSF21 | NNNCACATGTGGAGGAAAGG | NGG | 2 | 0.4615 | Tier II |
9 | NC_000006.12 | 6 | 157502185 | + | ZDHHC14 | NNNCACATCTGGTGGAAAGG | NGG | 2 | 0.359 | Tier II |
10 | NC_000010.11 | 10 | 103771163 | - | SH3PXD2A | NNNCACTTGTGGTGGAATAG | NGG | 2 | 0.3125 | Tier II |
11 | NC_000012.12 | 12 | 56551277 | + | RBMS2 | NNNCTCATGAGGTGGAATGG | NGG | 2 | 0.3117 | Tier II |
12 | NC_000016.10 | 16 | 12509795 | - | SNX29 | NNNCTCATGTGGCGGAATGG | NGG | 2 | 0.2871 | Tier II |
13 | NC_000009.12 | 9 | 35365803 | + | UNC13B | NNNCAGATGGGGTGGAATGG | NGG | 2 | 0.25 | Tier II |
14 | NC_000014.9 | 14 | 50460214 | - | MAP4K5 | NNNCACATGTGATGGAATGG | NAG | 2 | 0.242 | Tier II |
15 | NC_000003.12 | 3 | 76313522 | + | ROBO2 | NNNCACATGAGGTGGGATGG | NGG | 2 | 0.1648 | Tier II |
16 | NC_000005.10 | 5 | 130154407 | + | CHSY3 | NNNCACATGTCTTGGAATGG | NGG | 2 | 0.1648 | Tier II |
17 | NC_000014.9 | 14 | 61904211 | - | SYT16 | NNNCACATGTGGTATAATGG | NGG | 2 | 0.1071 | Tier II |
18 | NC_000011.10 | 11 | 11353024 | - | GALNT18 | NNNCACATGTGGTGGAATGG | NGA | 1 | 0.0694 | Tier II |
19 | NC_000014.9 | 14 | 94312323 | - | SERPINA6 | NNNCACATGTGGTGGAATGG | NTG | 1 | 0.039 | Tier II |
20 | NC_000017.11 | 17 | 73462316 | + | SDK2 | NNNCATATGTGGTGGAATGG | NTG | 2 | 0.0362 | Tier II |
21 | NC_000005.10 | 5 | 31802117 | + | PDZD2 | NNNCACATGTGGTGTGATGG | NGG | 2 | 0.0275 | Tier II |
22 | NC_000020.11 | 20 | 62279044 | + | OSBPL2 | NNNCACATGTTGTGGCATGG | NGG | 2 | 0.0 | Tier II |
23 | NC_000011.10 | 11 | 97273575 | - | LOC105369450 | NNNCATATGTGGTGGAAAGG | NGG | 2 | 0.619 | Tier III |
24 | NC_000015.10 | 15 | 93170819 | + | LOC101927025 | NNNGACATGTGGTGGAATGT | NGG | 2 | 0.35 | Tier III |
25 | NC_000008.11 | 8 | 11384704 | + | FAM167A-AS1 | NNNAACATGTGGTGGAATGG | NAG | 2 | 0.2183 | Tier III |
26 | NC_000005.10 | 5 | 174932857 | + | LINC01951 | NNNCTCATGTGGTGGAAGGG | NGG | 2 | 0.1212 | Tier III |
27 | NC_000005.10 | 5 | 117838020 | + | LINC02147 | NNNCACATCTGGTGGAATGG | NTG | 2 | 0.021 | Tier III |
28 | NC_000014.9 | 14 | 81661554 | + | EEF1A1P2 | NNNCAGATGTGGTGGTATGG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 152250607 | + | Csnk2a1 | NNNCACATGTGGTGGAATGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000071.6 | 5 | 121310567 | + | Hectd4 | NNNCACCTGTGGTGGGATGG | NGG | 2 | 0.0841 | Tier I |
3 | NC_000074.6 | 8 | 38577263 | + | Sgcz | NNNAACATGTGGTGGAATTG | NGG | 2 | 0.5614 | Tier II |
4 | NC_000086.7 | X | 157901840 | + | Cnksr2 | NNNAACATGTGCTGGAATGG | NGG | 2 | 0.4458 | Tier II |
5 | NC_000072.6 | 6 | 18017789 | + | Wnt2 | NNNTACATGTGCTGGAATGG | NGG | 2 | 0.4235 | Tier II |
6 | NC_000069.6 | 3 | 30348093 | - | Mecom | NNNCACATCTGGTGGAAAGG | NGG | 2 | 0.359 | Tier II |
7 | NC_000086.7 | X | 164346586 | + | Pir | NNNCACATGTGCTCGAATGG | NGG | 2 | 0.2269 | Tier II |
8 | NC_000068.7 | 2 | 42527628 | - | Lrp1b | NNNCACAGGTGGTTGAATGG | NGG | 2 | 0.1956 | Tier II |
9 | NC_000069.6 | 3 | 30259213 | + | Mecom | NNNCACATGTGGAGGAATGG | NAG | 2 | 0.1795 | Tier II |
10 | NC_000076.6 | 10 | 90533318 | + | Anks1b | NNNCACATGTGTTGGAATGG | NAG | 2 | 0.0997 | Tier II |
11 | NC_000070.6 | 4 | 118377854 | + | Szt2 | NNNAACATGTGGTGGAATGG | NGA | 2 | 0.0585 | Tier II |
12 | NC_000068.7 | 2 | 24734219 | - | Cacna1b | NNNCACAGGTGGTGGAATGG | NGT | 2 | 0.0118 | Tier II |
13 | NC_000068.7 | 2 | 24734256 | - | Cacna1b | NNNCACAGGTGGTGGAATGG | NGT | 2 | 0.0118 | Tier II |
14 | NC_000068.7 | 2 | 24734444 | - | Cacna1b | NNNCACAGGTGGTGGAATGG | NGT | 2 | 0.0118 | Tier II |
15 | NC_000068.7 | 2 | 24734863 | - | Cacna1b | NNNCACAGGTGGTGGAATGG | NGT | 2 | 0.0118 | Tier II |
16 | NC_000067.6 | 1 | 156524327 | + | Csnk2a3 | NNNCACATGTGGTGGAATGG | NGG | 0 | 1.0 | Tier III |
17 | NC_000082.6 | 16 | 5449713 | - | Gm41414 | NNNGACATGTGGAGGAATGG | NGG | 2 | 0.3462 | Tier III |
18 | NC_000075.6 | 9 | 47183223 | + | Gm31698 | NNNCACATGTGGTGGACTGA | NGG | 2 | 0.1654 | Tier III |
19 | NC_000074.6 | 8 | 99536411 | + | A330008L17Rik | NNNCACATGTGCTGGAGTGG | NGG | 2 | 0.0934 | Tier III |
20 | NC_000070.6 | 4 | 75324597 | + | Gm11255 | NNNCACATGTGATGGAATGG | NGA | 2 | 0.0648 | Tier III |
21 | NC_000086.7 | X | 16610348 | + | Csnk2a1-ps | NNNCACATGAGGTGGAATGG | NGA | 2 | 0.0595 | Tier III |
22 | NC_000080.6 | 14 | 14197400 | + | Csnk2a1-ps1 | NNNCACAGGTGGTGGAATGG | NGA | 2 | 0.0509 | Tier III |