Construct: sgRNA BRDN0001146324

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATAACGCTCTCCAGAACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHKA (1119)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76588

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 68074815 + CHKA NNNAACGCTCTCCAGAACCA NGG 0 1.0 Tier I
2 NC_000023.11 X 53534189 + HUWE1 NNNAATGCTCTCCAGAACCA NTG 2 0.0362 Tier I
3 NC_000016.10 16 68353979 + PRMT7 NNNTACGTTCTCCAGAACCA NGG 2 0.5568 Tier II
4 NC_000016.10 16 84767063 + USP10 NNNATCCCTCTCCAGAACCA NGG 2 0.25 Tier II
5 NC_000005.10 5 179558643 + RUFY1 NNNAACCCTCTCCACAACCA NGG 2 0.1875 Tier II
6 NC_000012.12 12 55750993 - GDF11 NNNAACGCTTTCCAGACCCA NGG 2 0.1661 Tier II
7 NC_000005.10 5 155976462 + SGCD NNNAACTCTCTCCAGAACCA NAG 2 0.1481 Tier II
8 NC_000022.11 22 31780669 - DEPDC5 NNNAAGGCTCTCCAGAAGCA NGG 2 0.0667 Tier II
9 NC_000004.12 4 68571870 - UGT2B17 NNNAACGCTCTCCAGAACCA NTG 1 0.039 Tier II
10 NC_000001.11 1 116965954 - PTGFRN NNNAACTCTCTCCAGAACCA NTG 2 0.0223 Tier II
11 NC_000005.10 5 139529737 + UBE2D2 NNNAACGCTCTCCAGAACCA NTA 2 0.0 Tier II
12 NC_000003.12 3 178278000 + LOC105374235 NNNTACCCTCTCCAGAACCA NGG 2 0.4375 Tier III
13 NC_000005.10 5 179558643 + LOC128966623 NNNAACCCTCTCCACAACCA NGG 2 0.1875 Tier III
14 NC_000008.11 8 88328140 - LOC105375630 NNNACCGCTCTCCAGGACCA NGG 2 0.0962 Tier III
15 NC_000002.12 2 13527733 - LOC105373485 NNNAACGCTCTCCAGAGCCA NCG 2 0.0189 Tier III
16 NC_000005.10 5 163314843 - LOC105377700 NNNAACGCTCTCCAGAACCA NTA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 88936017 - Ttll8 NNNAAAGCTCTCCAGAACCT NGG 2 0.5571 Tier I
2 NC_000070.6 4 24901841 + Ndufaf4 NNNAACTCTCTCCAGAAACA NGG 2 0.3077 Tier I
3 NC_000085.6 19 3884488 - Chka NNNAACACTCTCCAGAACCA NCG 2 0.1071 Tier I
4 NC_000085.6 19 8681202 + Chrm1 NNNAACACCCTCCAGAACCA NGG 2 0.9231 Tier II
5 NC_000075.6 9 117550493 - Rbms3 NNNAATGCCCTCCAGAACCA NGG 2 0.8571 Tier II
6 NC_000082.6 16 27412952 - Ccdc50 NNNAACACTCTCCTGAACCA NGG 2 0.5333 Tier II
7 NC_000084.6 18 42560756 - Tcerg1 NNNACAGCTCTCCAGAACCA NGG 2 0.4643 Tier II
8 NC_000068.7 2 7026414 - Celf2 NNNAACTCTCTCTAGAACCA NGG 2 0.4 Tier II
9 NC_000083.6 17 5647239 - Zdhhc14 NNNACCCCTCTCCAGAACCA NGG 2 0.3438 Tier II
10 NC_000076.6 10 11446768 - Epm2a NNNCATGCTCTCCAGAACCA NGG 2 0.3277 Tier II
11 NC_000082.6 16 13350405 - Mrtfb NNNAAGGGTCTCCAGAACCA NGG 2 0.3214 Tier II
12 NC_000072.6 6 84181746 + Dysf NNNAAGGCTCTCCAGAACAA NGG 2 0.2143 Tier II
13 NC_000073.6 7 140278399 - 5830411N06Rik NNNAACTCTCTCCAGAACCA NGA 2 0.0397 Tier II
14 NC_000073.6 7 16009406 - Bicra NNNAACCCTCTCCAGAACCA NGT 2 0.0111 Tier II
15 NC_000072.6 6 55056265 - Gars NNNCACGCTCTCCAGCACCA NGG 2 0.0 Tier II
16 NC_000079.6 13 118704042 + Gm16263 NNNAACTCTCTCCAGATCCA NGG 2 0.0762 Tier III
17 NC_000070.6 4 64252469 + Gm31115 NNNAACGCTCTCCAGTACCA NGC 2 0.0 Tier III
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Other clones with same target sequence:

(none)