Construct: sgRNA BRDN0001146328
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTATCTACCATACTACAAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK3 (9451)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77167
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
88586035 |
- |
EIF2AK3 |
NNNTCTACCATACTACAAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
72896894 |
- |
GXYLT2 |
NNNTCTACCTTACTACAAAA |
NGG |
2 |
0.6303 |
Tier II |
| 3 |
NC_000009.12 |
9 |
108871483 |
- |
ELP1 |
NNNTGTACCATACAACAAGA |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000019.10 |
19 |
37449753 |
- |
ZNF569 |
NNNACTACCAGACTACAAGA |
NGG |
2 |
0.1905 |
Tier II |
| 5 |
NC_000021.9 |
21 |
25977483 |
+ |
APP |
NNNTCTAACATACTTCAAGA |
NGG |
2 |
0.13 |
Tier II |
| 6 |
NC_000004.12 |
4 |
163919883 |
+ |
MARCHF1 |
NNNTCTACCATTCTACAAGA |
NAG |
2 |
0.0864 |
Tier II |
| 7 |
NC_000023.11 |
X |
71565925 |
- |
OGT |
NNNTCTACAATACTACAAGA |
NTG |
2 |
0.0334 |
Tier II |
| 8 |
NC_000016.10 |
16 |
53222863 |
- |
CHD9 |
NNNTCTACCATACTACAAAA |
NGT |
2 |
0.0115 |
Tier II |
| 9 |
NC_000003.12 |
3 |
100955620 |
+ |
ABI3BP |
NNNTCTACCATACAACAAGA |
NGT |
2 |
0.01 |
Tier II |
| 10 |
NC_000016.10 |
16 |
68815981 |
- |
CDH1 |
NNNTCTACCATACTACAAGA |
NCT |
2 |
0.0 |
Tier II |
| 11 |
NC_000004.12 |
4 |
133262674 |
+ |
LOC124900863 |
NNNTCTACCATCCTAGAAGA |
NGG |
2 |
0.0405 |
Tier III |
| 12 |
NC_000012.12 |
12 |
73163189 |
- |
LINC02444 |
NNNTCTACCATCCTAGAAGA |
NGG |
2 |
0.0405 |
Tier III |
| 13 |
NC_000001.11 |
1 |
187469872 |
- |
LINC01037 |
NNNTCTACCATACTAGAAGC |
NGG |
2 |
0.035 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
70885188 |
+ |
Eif2ak3 |
NNNTCTGCCATACTACAAGA |
NAG |
2 |
0.183 |
Tier I |
| 2 |
NC_000086.7 |
X |
100774303 |
- |
Dlg3 |
NNNTTTGCCATACTACAAGA |
NGG |
2 |
0.4492 |
Tier II |
| 3 |
NC_000074.6 |
8 |
83719136 |
- |
Ddx39a |
NNNTCTACCATACTACAAAT |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000068.7 |
2 |
135094043 |
- |
Plcb1 |
NNNGCTACCACACTACAAGA |
NGG |
2 |
0.4167 |
Tier II |
| 5 |
NC_000074.6 |
8 |
15998030 |
- |
Csmd1 |
NNNTCTACCATTCTAGAAGA |
NGG |
2 |
0.0513 |
Tier II |
| 6 |
NC_000076.6 |
10 |
19192985 |
- |
Gm20139 |
NNNTCTACCAAGCTACAAGA |
NGG |
2 |
0.5417 |
Tier III |
Other clones with same target sequence:
(none)
