Construct: sgRNA BRDN0001146329
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCACTCGTACAGACTCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAR1A (5573)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77720
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
68522808 |
+ |
PRKAR1A |
NNNCACTCGTACAGACTCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
467816 |
+ |
ZNF721 |
NNNCACTCGTACACACTCAG |
NGG |
2 |
0.3277 |
Tier II |
| 3 |
NC_000004.12 |
4 |
467816 |
+ |
ABCA11P |
NNNCACTCGTACACACTCAG |
NGG |
2 |
0.3277 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
101259126 |
+ |
Vps25 |
NNNCACTCCTCCAGACTCAA |
NGG |
2 |
0.2154 |
Tier II |
| 2 |
NC_000077.6 |
11 |
70869035 |
- |
Rabep1 |
NNNCACTCTTTCAGACTCAA |
NGG |
2 |
0.1641 |
Tier II |
| 3 |
NC_000070.6 |
4 |
128951475 |
+ |
Azin2 |
NNNCACTGGTACAGACTCAC |
NGG |
2 |
0.1461 |
Tier II |
| 4 |
NC_000076.6 |
10 |
14464445 |
- |
Adgrg6 |
NNNCACTCTTACAGACTCAA |
NGC |
2 |
0.0119 |
Tier II |
| 5 |
NC_000069.6 |
3 |
64738958 |
+ |
Vmn2r-ps11 |
NNNCATTTGTACAGACTCAA |
NGG |
2 |
0.8125 |
Tier III |
Other clones with same target sequence:
(none)
