Construct: sgRNA BRDN0001146339
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTATCGTGGAAGTCACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CLK4 (57396)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77800
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
178623336 |
- |
CLK4 |
NNNATCGTGGAAGTCACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
17884599 |
- |
SNX13 |
NNNAACATGGAAGTCACAAG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000020.11 |
20 |
21219061 |
+ |
KIZ |
NNNATCTGGGAAGTCACAAG |
NGG |
2 |
0.419 |
Tier II |
| 4 |
NC_000003.12 |
3 |
130385587 |
+ |
COL6A5 |
NNNATCTTGGAAGTCATAAG |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000006.12 |
6 |
45957088 |
+ |
CLIC5 |
NNNATGGTGGAAGTAACAAG |
NGG |
2 |
0.1111 |
Tier II |
| 6 |
NC_000005.10 |
5 |
126980202 |
+ |
MARCHF3 |
NNNATCCTGGAAGTGACAAG |
NGG |
2 |
0.0344 |
Tier II |
| 7 |
NC_000011.10 |
11 |
126668259 |
+ |
KIRREL3 |
NNNATCGTGGAAGGGACAAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000012.12 |
12 |
38885923 |
- |
CPNE8 |
NNNATGGTGGAAGGCACAAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000012.12 |
12 |
84071161 |
- |
LOC107984536 |
NNNATCGTGGAAGACACCAG |
NGG |
2 |
0.1179 |
Tier III |
| 10 |
NC_000011.10 |
11 |
126668259 |
+ |
LOC101929427 |
NNNATCGTGGAAGGGACAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
105814846 |
+ |
Lrrc8d |
NNNATCGTTGAAGACACAAG |
NGG |
2 |
0.3302 |
Tier II |
| 2 |
NC_000071.6 |
5 |
88591408 |
- |
Rufy3 |
NNNAGCGTGGAAGTCACAGG |
NGG |
2 |
0.24 |
Tier II |
| 3 |
NC_000068.7 |
2 |
162284757 |
- |
Ptprt |
NNNATCGTGGAAGTCAGAAC |
NGG |
2 |
0.0252 |
Tier II |
| 4 |
NC_000074.6 |
8 |
127801533 |
+ |
Gm32590 |
NNNATCAAGGAAGTCACAAG |
NGG |
2 |
0.8 |
Tier III |
| 5 |
NC_000075.6 |
9 |
103950090 |
+ |
Gm29154 |
NNNAACGAGGAAGTCACAAG |
NGG |
2 |
0.4 |
Tier III |
Other clones with same target sequence:
(none)
