Construct: sgRNA BRDN0001146341
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATCCCGAGACACGGTGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN1 (2081)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76408
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
64057952 |
+ |
ERN1 |
NNNTCCCGAGACACGGTGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
207649521 |
+ |
CR1L |
NNNTCCCGAGACACAGTAGT |
NGG |
2 |
0.6516 |
Tier II |
| 3 |
NC_000017.11 |
17 |
39672135 |
+ |
PGAP3 |
NNNTCCCGACACACGGTGGC |
NGG |
2 |
0.0364 |
Tier II |
| 4 |
NC_000001.11 |
1 |
207649521 |
+ |
CD46P1 |
NNNTCCCGAGACACAGTAGT |
NGG |
2 |
0.6516 |
Tier III |
| 5 |
NC_000012.12 |
12 |
47837358 |
+ |
LINC02354 |
NNNTACCCAGACACGGTGGT |
NGG |
2 |
0.3516 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
106411773 |
+ |
Ern1 |
NNNTCCTGAGATACGGTGGT |
NGG |
2 |
0.4375 |
Tier I |
| 2 |
NC_000072.6 |
6 |
146627020 |
- |
Tm7sf3 |
NNNGCCCGAGACACGGTGGT |
NGG |
1 |
0.625 |
Tier II |
| 3 |
NC_000085.6 |
19 |
58802822 |
+ |
Hspa12a |
NNNTCCAGAGACACGGTGGT |
NAG |
2 |
0.1944 |
Tier II |
| 4 |
NC_000071.6 |
5 |
148213677 |
- |
Mtus2 |
NNNTCCTGAGACACGGGGGT |
NGG |
2 |
0.0956 |
Tier II |
| 5 |
NC_000068.7 |
2 |
109955402 |
- |
Lgr4 |
NNNTACCGAGACACTGTGGT |
NGG |
2 |
0.0816 |
Tier II |
| 6 |
NC_000073.6 |
7 |
134777809 |
+ |
Dock1 |
NNNTCCTGAGACACGGTGGT |
NTG |
2 |
0.0317 |
Tier II |
| 7 |
NC_000077.6 |
11 |
110542449 |
- |
Gm11682 |
NNNTCCTGAGACACAGTGGT |
NGG |
2 |
0.7647 |
Tier III |
Other clones with same target sequence:
(none)
