Construct: sgRNA BRDN0001146344

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACGTGGTGAGACTCCACTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMKK2 (10645)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76697

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 121274092 + CAMKK2 NNNTGGTGAGACTCCACTGT NGG 0 1.0 Tier I
2 NC_000001.11 1 198187693 + NEK7 NNNTGGTGAGAATCCACTGT NGG 1 0.7143 Tier II
3 NC_000001.11 1 165270934 - LMX1A NNNTGGTGAGGCTCCACTGT NGG 1 0.65 Tier II
4 NC_000001.11 1 50184816 - ELAVL4 NNNTAGTCAGACTCCACTGT NGG 2 0.5333 Tier II
5 NC_000001.11 1 154774379 - KCNN3 NNNTGGTGGGACTCCACAGT NGG 2 0.4444 Tier II
6 NC_000006.12 6 89644455 - MDN1 NNNTGGGGAGACACCACTGT NGG 2 0.4072 Tier II
7 NC_000016.10 16 83719934 + CDH13 NNNGGGTCAGACTCCACTGT NGG 2 0.3846 Tier II
8 NC_000010.11 10 76523467 + LRMDA NNNGGGTGAGATTCCACTGT NGG 2 0.3365 Tier II
9 NC_000023.11 X 101242027 + DRP2 NNNTGCTGAGACTCCATTGT NGG 2 0.3182 Tier II
10 NC_000009.12 9 72146512 - GDA NNNGGGTGAGACTCCACTCT NGG 2 0.2802 Tier II
11 NC_000020.11 20 3900560 + PANK2 NNNTGGTGTGACTCCATTGT NGG 2 0.28 Tier II
12 NC_000005.10 5 141605826 - DIAPH1 NNNTGGTGAGAAGCCACTGT NGG 2 0.1863 Tier II
13 NC_000012.12 12 62399871 - USP15 NNNTGGTGAAACTCCACTGG NGG 2 0.1647 Tier II
14 NC_000007.14 7 137621898 - DGKI NNNTGGTGAGACCCCACTGG NGG 2 0.1393 Tier II
15 NC_000005.10 5 14703509 + OTULIN NNNTGGTGGGACTCCACTGG NGG 2 0.1176 Tier II
16 NC_000006.12 6 142328688 - ADGRG6 NNNTGGTGATACTCTACTGT NGG 2 0.0542 Tier II
17 NC_000007.14 7 66666261 - RABGEF1 NNNTGGAGAGACTCCACTGT NGT 2 0.0141 Tier II
18 NC_000011.10 11 114786970 + NXPE2 NNNTGGTGAGACTGCACTGT NGG 1 0.0 Tier II
19 NC_000001.11 1 233639882 + KCNK1 NNNTGGTGGGACTCCTCTGT NGG 2 0.0 Tier II
20 NC_000009.12 9 38213505 + LOC107987064 NNNTGGTGTGACTCCACTGT NGG 1 0.6 Tier III
21 NC_000006.12 6 89644455 - MDN1-AS1 NNNTGGGGAGACACCACTGT NGG 2 0.4072 Tier III
22 NC_000003.12 3 187486008 - LOC124909471 NNNTGTTGAGACTCCACTGT NAG 2 0.1728 Tier III
23 NC_000007.14 7 66666261 - LOC100996437 NNNTGGAGAGACTCCACTGT NGT 2 0.0141 Tier III
24 NC_000013.11 13 104711961 - LOC107984624 NNNTGGTGAGACTCCACTTT NGT 2 0.0108 Tier III
25 NC_000011.10 11 114786970 + LOC105369506 NNNTGGTGAGACTGCACTGT NGG 1 0.0 Tier III
26 NC_000023.11 X 40370072 + LOC105373182 NNNTTGTGAGACTCCTCTGT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 65395906 - Zfp395 NNNAGGTGAGACTTCACTGT NGG 2 0.3492 Tier I
2 NC_000071.6 5 122763810 + Camkk2 NNNTGGTGTGACTCTACTGT NGG 2 0.04 Tier I
3 NC_000067.6 1 180995002 + Ephx1 NNNGGATGAGACTCCACTGT NGG 2 0.625 Tier II
4 NC_000084.6 18 37926011 - Diaph1 NNNTGGTGAGAAACCACTGT NGG 2 0.4945 Tier II
5 NC_000077.6 11 77529520 + Taok1 NNNTGGTAAGCCTCCACTGT NGG 2 0.4 Tier II
6 NC_000075.6 9 119706203 - Scn10a NNNTGGTGTGACTCCACTTT NGG 2 0.4 Tier II
7 NC_000080.6 14 103122696 + Mycbp2 NNNTGGGGAGACTCCAATGT NGG 2 0.2745 Tier II
8 NC_000071.6 5 102370036 + Arhgap24 NNNTGGGGAGAGTCCACTGT NGG 2 0.2614 Tier II
9 NC_000072.6 6 8299352 - Umad1 NNNTCGTGAGACTCAACTGT NGG 2 0.1746 Tier II
10 NC_000079.6 13 10196008 - Chrm3 NNNTAGTGAGACTCCACTGG NGG 2 0.1529 Tier II
11 NC_000073.6 7 3220786 + Nlrp12 NNNTGGTGAGACTCAAATGT NGG 2 0.1037 Tier II
12 NC_000078.6 12 117705311 + Rapgef5 NNNTGGTGAGATTCCACTGC NGG 2 0.049 Tier II
13 NC_000067.6 1 43489059 + Nck2 NNNTGGTGAGACTCAACTGG NGG 2 0.0392 Tier II
14 NC_000083.6 17 73155857 + Lclat1 NNNTGGTGGGACTCCAGTGT NGG 2 0.0392 Tier II
15 NC_000084.6 18 66247442 - Ccbe1 NNNTGGTGAGACTCCACTGA NGA 2 0.0391 Tier II
16 NC_000081.6 15 8694672 + Slc1a3 NNNTGGTGAGAATCCACTGT NTG 2 0.0278 Tier II
17 NC_000076.6 10 20416779 - Pde7b NNNTGGTGAGCCTCCAGTGT NGG 2 0.0235 Tier II
18 NC_000069.6 3 7600087 - Il7 NNNTGGTGAGTCTCTACTGT NGG 2 0.0205 Tier II
19 NC_000071.6 5 65025497 - Fam114a1 NNNTGGTGAGACTCCATTGT NGC 2 0.0104 Tier II
20 NC_000073.6 7 64619224 + Apba2 NNNTGATGAGACTCCTCTGT NGG 2 0.0 Tier II
21 NC_000081.6 15 86339225 + Tbc1d22a NNNTGGTGAGACCCCTCTGT NGG 2 0.0 Tier II
22 NC_000068.7 2 44575328 + Gtdc1 NNNTGGTGAGACTCCACTGT NTT 2 0.0 Tier II
23 NC_000069.6 3 32903586 + Usp13 NNNTGGTGAGACTCCTCTTT NGG 2 0.0 Tier II
24 NC_000067.6 1 36217517 + Uggt1 NNNTGGTGAGACTCGCCTGT NGG 2 0.0 Tier II
25 NC_000070.6 4 49206406 - Plppr1 NNNTGGTGAGACTGCACTGT NAG 2 0.0 Tier II
26 NC_000073.6 7 67579298 - Lrrc28 NNNTGGTGAGACTGCACTGT NAG 2 0.0 Tier II
27 NC_000079.6 13 57752751 - Spock1 NNNTGGTGAGACTTCTCTGT NGG 2 0.0 Tier II
28 NC_000079.6 13 99523052 + Gm26559 NNNTGATGAGAATCCACTGT NGG 2 0.7143 Tier III
29 NC_000077.6 11 71250764 + Nlrp1c-ps NNNTGGTGAAACTCCACTGG NGG 2 0.1647 Tier III
30 NC_000073.6 7 3220786 + Mir295 NNNTGGTGAGACTCAAATGT NGG 2 0.1037 Tier III
31 NC_000073.6 7 3220786 + D7Ertd143e NNNTGGTGAGACTCAAATGT NGG 2 0.1037 Tier III
32 NC_000067.6 1 88450741 + Gm29538 NNNTGCTGAGACTCCACTGT NGC 2 0.0152 Tier III
33 NC_000085.6 19 12595777 + A330040F15Rik NNNTGGTGAGACTCAAGTGT NGG 2 0.0131 Tier III
34 NC_000075.6 9 48975270 - Gm51673 NNNTGGTCAGACTCCCCTGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)