Construct: sgRNA BRDN0001146352
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGCTCCGAGGTAAGGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UCK2 (7371)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75767
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
165890305 |
- |
UCK2 |
NNNGCTCCGAGGTAAGGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43520730 |
+ |
MAP1A |
NNNGCTCTGAGGTAAGGCCA |
NGG |
2 |
0.1667 |
Tier I |
| 3 |
NC_000011.10 |
11 |
64833919 |
- |
CDC42BPG |
NNNGCTCAGAGGTAAGGACC |
NGG |
2 |
0.1477 |
Tier I |
| 4 |
NC_000017.11 |
17 |
1543044 |
+ |
PITPNA |
NNNGCTCCGAGGCAAGGACA |
NGG |
1 |
0.7895 |
Tier II |
| 5 |
NC_000016.10 |
16 |
66685587 |
- |
CMTM4 |
NNNGCTTAGAGGTAAGGACA |
NGG |
2 |
0.5281 |
Tier II |
| 6 |
NC_000016.10 |
16 |
82810141 |
+ |
CDH13 |
NNNGCTTCAAGGTAAGGACA |
NGG |
2 |
0.5223 |
Tier II |
| 7 |
NC_000019.10 |
19 |
36103131 |
- |
WDR62 |
NNNGCTCTGAGGTGAGGACA |
NGG |
2 |
0.4083 |
Tier II |
| 8 |
NC_000011.10 |
11 |
1437484 |
- |
BRSK2 |
NNNGCTCCGAGGTAAGGGCA |
NGG |
1 |
0.4 |
Tier II |
| 9 |
NC_000003.12 |
3 |
128322322 |
- |
EEFSEC |
NNNGCTCCCAGGTAGGGACA |
NGG |
2 |
0.35 |
Tier II |
| 10 |
NC_000007.14 |
7 |
158397252 |
+ |
PTPRN2 |
NNNCCTCCGAGGTAGGGACA |
NGG |
2 |
0.3441 |
Tier II |
| 11 |
NC_000002.12 |
2 |
69538984 |
- |
AAK1 |
NNNGCTCAGAGGTGAGGACA |
NGG |
2 |
0.3033 |
Tier II |
| 12 |
NC_000006.12 |
6 |
142878878 |
+ |
HIVEP2 |
NNNGCTCCAAGGTGAGGACA |
NGG |
2 |
0.3 |
Tier II |
| 13 |
NC_000001.11 |
1 |
44300531 |
+ |
ERI3 |
NNNGCTCTGAGGGAAGGACA |
NGG |
2 |
0.2283 |
Tier II |
| 14 |
NC_000014.9 |
14 |
104885766 |
+ |
CEP170B |
NNNGCTCTGAGGTCAGGACA |
NGG |
2 |
0.1875 |
Tier II |
| 15 |
NC_000007.14 |
7 |
551770 |
- |
PRKAR1B |
NNNGCTCCGAGGGAAGGACC |
NGG |
2 |
0.0593 |
Tier II |
| 16 |
NC_000016.10 |
16 |
82810141 |
+ |
LOC101928446 |
NNNGCTTCAAGGTAAGGACA |
NGG |
2 |
0.5223 |
Tier III |
| 17 |
NC_000013.11 |
13 |
111672280 |
+ |
LOC102724489 |
NNNGCTCAGAGGTCAGGACA |
NGG |
2 |
0.1393 |
Tier III |
| 18 |
NC_000013.11 |
13 |
111757194 |
+ |
LOC102724510 |
NNNGCTCAGAGGTCAGGACA |
NGG |
2 |
0.1393 |
Tier III |
| 19 |
NC_000022.11 |
22 |
49600565 |
+ |
MIR3667HG |
NNNGCTCCGAGGGAAGGACA |
NGA |
2 |
0.0181 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
47650251 |
- |
Col17a1 |
NNNGCTCCGAGGTAAGGCCA |
NGC |
2 |
0.0042 |
Tier I |
| 2 |
NC_000076.6 |
10 |
77064718 |
+ |
Col18a1 |
NNNGCTCTGAAGTAAGGACA |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000068.7 |
2 |
179837271 |
- |
Cdh4 |
NNNGCTCAGAGGTAAGAACA |
NGG |
2 |
0.6067 |
Tier II |
| 4 |
NC_000078.6 |
12 |
116944994 |
- |
Ptprn2 |
NNNGCTCAGAGGCAAGGACA |
NGG |
2 |
0.5132 |
Tier II |
| 5 |
NC_000077.6 |
11 |
75608091 |
- |
Pitpna |
NNNGCTCCAAGGCAAGGACA |
NGG |
2 |
0.5075 |
Tier II |
| 6 |
NC_000068.7 |
2 |
168727511 |
+ |
Atp9a |
NNNCCTCTGAGGTAAGGACA |
NGG |
2 |
0.4632 |
Tier II |
| 7 |
NC_000077.6 |
11 |
108760696 |
- |
Cep112 |
NNNGATCCCAGGTAAGGACA |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000069.6 |
3 |
82843687 |
+ |
Rbm46 |
NNNGCTCAGAGGTAAGGAAA |
NGG |
2 |
0.2786 |
Tier II |
| 9 |
NC_000076.6 |
10 |
13008590 |
- |
Sf3b5 |
NNNGCTCCGAGGTCAGGACA |
NAG |
2 |
0.0556 |
Tier II |
| 10 |
NC_000085.6 |
19 |
25525422 |
+ |
Dmrt1 |
NNNGCTTCGAGGTAAGGACA |
NTG |
2 |
0.0317 |
Tier II |
| 11 |
NC_000067.6 |
1 |
132055010 |
- |
Mfsd4a |
NNNGCTCAGAGGTAAGGACA |
NGC |
2 |
0.0144 |
Tier II |
Other clones with same target sequence:
(none)
