Construct: sgRNA BRDN0001146353

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTGTGAAAGGTTTATACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIP5K2 (23262)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76340

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 103149159 + PPIP5K2 NNNGTGAAAGGTTTATACAG NGG 0 1.0 Tier I
2 NC_000020.11 20 45096758 - KCNS1 NNNGTGAGAGGTTTAAACAG NGG 2 0.6667 Tier II
3 NC_000004.12 4 16876849 - LDB2 NNNGTTAAAGGTTTATACAA NGG 2 0.625 Tier II
4 NC_000013.11 13 41228727 - MTRF1 NNNGTTAAATGTTTATACAG NGG 2 0.5417 Tier II
5 NC_000023.11 X 105888029 + NRK NNNGTGAAAATTTTATACAG NGG 2 0.359 Tier II
6 NC_000007.14 7 135383328 - CNOT4 NNNGTGAAAGATTTAGACAG NGG 2 0.3462 Tier II
7 NC_000010.11 10 86868061 + BMPR1A NNNGTGGAAGTTTTATACAG NGG 2 0.2715 Tier II
8 NC_000001.11 1 202420412 + PPP1R12B NNNGTGAAAGGTTTCTACAA NGG 2 0.2557 Tier II
9 NC_000001.11 1 51628142 + OSBPL9 NNNGTGAAAGGTCTATACAG NAG 2 0.2047 Tier II
10 NC_000008.11 8 89974654 + NBN NNNGTGAAAGGTACATACAG NGG 2 0.1978 Tier II
11 NC_000006.12 6 65323853 - EYS NNNGTGAAAGGTTTAAGCAG NGG 2 0.1604 Tier II
12 NC_000001.11 1 108971709 + WDR47 NNNTTGAAAGGTTTATACAG NGA 2 0.0253 Tier II
13 NC_000003.12 3 42579957 + SEC22C NNNGAGAAAGGTTTATACAG NTG 2 0.0195 Tier II
14 NC_000003.12 3 19489004 + KCNH8 NNNGTGAAAGGTTGATACAA NGG 2 0.0 Tier II
15 NC_000001.11 1 67729547 - GNG12 NNNGTGAAAGGTTGATGCAG NGG 2 0.0 Tier II
16 NC_000007.14 7 7891441 + LOC124901586 NNNGTGAAATGTTTATACAA NGG 2 0.7617 Tier III
17 NC_000013.11 13 41228727 - KBTBD6-DT NNNGTTAAATGTTTATACAG NGG 2 0.5417 Tier III
18 NC_000001.11 1 50304541 + LINC02808 NNNTTGAAAAGTTTATACAG NGG 2 0.3394 Tier III
19 NC_000008.11 8 104887179 - LOC105375694 NNNGTGAAATGTTTATTCAG NGG 2 0.1083 Tier III
20 NC_000020.11 20 35558760 + FER1L4 NNNGTGATAGGTTTATTCAG NGG 2 0.0571 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 156078902 + Fam163a NNNGTGAAAGGATTATACAT NGG 2 0.56 Tier I
2 NC_000067.6 1 97750722 - Ppip5k2 NNNATGAAAGGTTTATACCG NGG 2 0.1862 Tier I
3 NC_000074.6 8 111170537 + Glg1 NNNGGGAAAGGTTTATACAA NGG 2 0.6 Tier II
4 NC_000068.7 2 76113356 - Pde11a NNNGTGAAAGGATTACACAG NGG 2 0.5333 Tier II
5 NC_000080.6 14 21241762 + Adk NNNGTGTAAGGCTTATACAG NGG 2 0.4145 Tier II
6 NC_000072.6 6 46435191 - Cntnap2 NNNATGTAAGGTTTATACAG NGG 2 0.3938 Tier II
7 NC_000068.7 2 44740129 + Gtdc1 NNNGTGAAAGGGTTATACAT NGG 2 0.35 Tier II
8 NC_000068.7 2 72184609 + Rapgef4 NNNGTGAAAGGTTTAGACAT NGG 2 0.2423 Tier II
9 NC_000069.6 3 157299261 - Negr1 NNNGTTAAAGGTTTAGACAG NGG 2 0.2308 Tier II
10 NC_000067.6 1 64080218 - Klf7 NNNGTGAAAGGTTTATGCTG NGG 2 0.095 Tier II
11 NC_000081.6 15 64120539 - Asap1 NNNGTGAAAGGTTTGTACAG NGA 2 0.0451 Tier II
12 NC_000077.6 11 32352481 + Sh3pxd2b NNNGTGAAAGGTTTATACAT NTG 2 0.0273 Tier II
13 NC_000072.6 6 98987348 - Foxp1 NNNGAGAAAGGTTTATACAG NGC 2 0.0111 Tier II
14 NC_000083.6 17 56981262 + Acer1 NNNGTGAAAGGTTTCTACAG NGT 2 0.0044 Tier II
15 NC_000070.6 4 80587989 + Gm32658 NNNGAGAAAGGATTATACAG NGG 2 0.4 Tier III
16 NC_000067.6 1 64080218 - Gm13748 NNNGTGAAAGGTTTATGCTG NGG 2 0.095 Tier III
17 NC_000084.6 18 51565103 + Gm41722 NNNGTGAAAGCTTTATACAG NGA 2 0.0298 Tier III
Download CSV

Other clones with same target sequence:

(none)