Construct: sgRNA BRDN0001146359
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCCCTGTCTCGACAGCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK4 (6787)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77712
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
52746806 |
- |
NEK4 |
NNNCCCTGTCTCGACAGCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
155318795 |
+ |
FDPS |
NNNCCATGTCTGGACAGCGA |
NGG |
2 |
0.4127 |
Tier II |
| 3 |
NC_000019.10 |
19 |
4064359 |
+ |
ZBTB7A |
NNNCTCTGTCTCCACAGCGA |
NGG |
2 |
0.2679 |
Tier II |
| 4 |
NC_000023.11 |
X |
104106371 |
- |
SLC25A53 |
NNNCCCTGTCTCGTCAGCCA |
NGG |
2 |
0.2391 |
Tier II |
| 5 |
NC_000004.12 |
4 |
2795910 |
+ |
SH3BP2 |
NNNCCCTGTCTGGACACCGA |
NGG |
2 |
0.1046 |
Tier II |
| 6 |
NC_000006.12 |
6 |
114152796 |
+ |
HS3ST5 |
NNNCCCTGTCTCTACTGCGA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000001.11 |
1 |
155318795 |
+ |
RUSC1-AS1 |
NNNCCATGTCTGGACAGCGA |
NGG |
2 |
0.4127 |
Tier III |
| 8 |
NC_000006.12 |
6 |
114152796 |
+ |
HDAC2-AS2 |
NNNCCCTGTCTCTACTGCGA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
65084900 |
- |
Nedd4l |
NNNCCCTGACTCAACAGCGA |
NGG |
2 |
0.8571 |
Tier II |
| 2 |
NC_000081.6 |
15 |
57995705 |
- |
Fam83a |
NNNCCCTGTCTCAACAGCTA |
NGG |
2 |
0.6154 |
Tier II |
| 3 |
NC_000073.6 |
7 |
125845326 |
+ |
D430042O09Rik |
NNNCCTTGTCTCGACAGAGA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000075.6 |
9 |
67411377 |
- |
Tln2 |
NNNCACTGTCTCGACAGCAA |
NGG |
2 |
0.4082 |
Tier II |
| 5 |
NC_000071.6 |
5 |
117183210 |
+ |
Taok3 |
NNNCCCTGTCTCGACAGAGA |
NAG |
2 |
0.1396 |
Tier II |
| 6 |
NC_000068.7 |
2 |
126751514 |
- |
Usp8 |
NNNCCCTGTCTCTACACCGA |
NGG |
2 |
0.0706 |
Tier II |
| 7 |
NC_000075.6 |
9 |
106274560 |
+ |
Gm33564 |
NNNCCCTGTCTCAACAACGA |
NGG |
2 |
0.8615 |
Tier III |
| 8 |
NC_000074.6 |
8 |
125659176 |
- |
Gm30871 |
NNNCCCTGTCTAGACAGCAA |
NGG |
2 |
0.5102 |
Tier III |
| 9 |
NC_000074.6 |
8 |
30948070 |
- |
Gm32791 |
NNNCCCTCTCTCGACAGCAA |
NGG |
2 |
0.4396 |
Tier III |
Other clones with same target sequence:
(none)
