Construct: sgRNA BRDN0001146364
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTAGAAATTCGAGCCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMX (660)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75653
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
15531365 |
+ |
BMX |
NNNTAGAAATTCGAGCCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
15531365 |
+ |
ACE2 |
NNNTAGAAATTCGAGCCAAG |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000022.11 |
22 |
43702808 |
- |
EFCAB6 |
NNNTATAAATTCAAGCCAAG |
NGG |
2 |
0.6154 |
Tier II |
| 4 |
NC_000001.11 |
1 |
233191897 |
+ |
PCNX2 |
NNNTAGAAGTTAGAGCCAAG |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000005.10 |
5 |
177426451 |
+ |
GRK6 |
NNNTAGAAATTCGAACCTAG |
NGG |
2 |
0.4706 |
Tier II |
| 6 |
NC_000008.11 |
8 |
130812563 |
+ |
ADCY8 |
NNNGAGAAATTAGAGCCAAG |
NGG |
2 |
0.4464 |
Tier II |
| 7 |
NC_000010.11 |
10 |
24381312 |
+ |
KIAA1217 |
NNNTAGCAATTCAAGCCAAG |
NGG |
2 |
0.4038 |
Tier II |
| 8 |
NC_000004.12 |
4 |
23943416 |
- |
PPARGC1A |
NNNTATAAATTTGAGCCAAG |
NGG |
2 |
0.359 |
Tier II |
| 9 |
NC_000007.14 |
7 |
8147681 |
+ |
ICA1 |
NNNTAGAATTTTGAGCCAAG |
NGG |
2 |
0.3231 |
Tier II |
| 10 |
NC_000012.12 |
12 |
128414612 |
+ |
TMEM132C |
NNNTAAAAATTCTAGCCAAG |
NGG |
2 |
0.3 |
Tier II |
| 11 |
NC_000012.12 |
12 |
57040745 |
+ |
MYO1A |
NNNTAGAAATTGGAGCCATG |
NGG |
2 |
0.2393 |
Tier II |
| 12 |
NC_000020.11 |
20 |
56367353 |
+ |
FAM210B |
NNNTAGAAATTCGAGCATAG |
NGG |
2 |
0.2333 |
Tier II |
| 13 |
NC_000006.12 |
6 |
84161352 |
+ |
CEP162 |
NNNTAGATATTTGAGCCAAG |
NGG |
2 |
0.2308 |
Tier II |
| 14 |
NC_000014.9 |
14 |
81121045 |
+ |
TSHR |
NNNTAGAAATTCAAGCCACG |
NGG |
2 |
0.191 |
Tier II |
| 15 |
NC_000014.9 |
14 |
72055830 |
- |
RGS6 |
NNNTAGAAATTAGAGGCAAG |
NGG |
2 |
0.1099 |
Tier II |
| 16 |
NC_000005.10 |
5 |
145358222 |
- |
PRELID2 |
NNNTAGAAATTCCAGCCCAG |
NGG |
2 |
0.0802 |
Tier II |
| 17 |
NC_000015.10 |
15 |
47843595 |
- |
LINC01491 |
NNNTAGAAATTGGAGACAAG |
NGG |
2 |
0.4444 |
Tier III |
| 18 |
NC_000017.11 |
17 |
14057405 |
+ |
COX10-DT |
NNNTAGAAATTATAGCCAAG |
NGG |
2 |
0.2143 |
Tier III |
| 19 |
NC_000014.9 |
14 |
81121045 |
+ |
LOC101928462 |
NNNTAGAAATTCAAGCCACG |
NGG |
2 |
0.191 |
Tier III |
| 20 |
NC_000011.10 |
11 |
50207593 |
+ |
LOC124902811 |
NNNTAGAAATTCCAGCCAGG |
NGG |
2 |
0.1579 |
Tier III |
| 21 |
NC_000001.11 |
1 |
69434538 |
+ |
LINC01758 |
NNNTAGAAATTCTAGCCTAG |
NGG |
2 |
0.15 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
102566680 |
- |
Atf7 |
NNNTAGAAATTAGAGACAAG |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000071.6 |
5 |
143649253 |
+ |
Cyth3 |
NNNTAGAAATTAGAGCCAAA |
NGG |
2 |
0.6696 |
Tier II |
| 3 |
NC_000079.6 |
13 |
55445115 |
+ |
Grk6 |
NNNTAGAAATTCGAACCTAG |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000067.6 |
1 |
159728184 |
- |
Tnr |
NNNAAGAAATTTGAGCCAAG |
NGG |
2 |
0.2564 |
Tier II |
| 5 |
NC_000077.6 |
11 |
42614138 |
- |
Gabrb2 |
NNNTAGAAATTAGAGCCAAG |
NGA |
2 |
0.0496 |
Tier II |
| 6 |
NC_000072.6 |
6 |
95117223 |
+ |
Kbtbd8os |
NNNTAGAAATGCAAGCCAAG |
NGG |
2 |
0.3692 |
Tier III |
Other clones with same target sequence:
(none)
