Construct: sgRNA BRDN0001146368
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACTAATCTACCATCCGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- C17orf75 (64149)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78078
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
32339907 |
- |
C17orf75 |
NNNTAATCTACCATCCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
16272274 |
+ |
OXNAD1 |
NNNTAATCTACCACCTGAAG |
NGG |
2 |
0.0879 |
Tier I |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
80375601 |
- |
5730455P16Rik |
NNNTAATCTACCATCTGAAG |
NGG |
1 |
0.3077 |
Tier I |
| 2 |
NC_000083.6 |
17 |
86549424 |
+ |
Prkce |
NNNTCATCTGCCATCCGAAG |
NGG |
2 |
0.2778 |
Tier II |
| 3 |
NC_000071.6 |
5 |
108271094 |
- |
Dr1 |
NNNTAATCTACCATCAGCAG |
NGG |
2 |
0.1905 |
Tier II |
| 4 |
NC_000067.6 |
1 |
79756105 |
- |
Wdfy1 |
NNNTAATCTACCTTCCGAAG |
NGT |
2 |
0.0048 |
Tier II |
| 5 |
NC_000070.6 |
4 |
77333542 |
+ |
Ptprd |
NNNTAATCTAGCATCCGAAG |
NGT |
2 |
0.004 |
Tier II |
| 6 |
NC_000079.6 |
13 |
83112416 |
- |
Gm33073 |
NNNTAAGCTACCATCAGAAG |
NGG |
2 |
0.5882 |
Tier III |
| 7 |
NC_000067.6 |
1 |
103243236 |
- |
Gm18728 |
NNNTATTCTACCACCCGAAG |
NGG |
2 |
0.2041 |
Tier III |
| 8 |
NC_000073.6 |
7 |
142669354 |
- |
Igf2os |
NNNTAATCTACCATGCAAAG |
NGG |
2 |
0.0467 |
Tier III |
| 9 |
NC_000077.6 |
11 |
45714570 |
+ |
Gm35282 |
NNNTAATCTACCATCCTAAG |
NGA |
2 |
0.0174 |
Tier III |
| 10 |
NC_000077.6 |
11 |
45714570 |
+ |
Gm39816 |
NNNTAATCTACCATCCTAAG |
NGA |
2 |
0.0174 |
Tier III |
Other clones with same target sequence:
(none)
