Construct: sgRNA BRDN0001146377
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGGGCTCGTCCATGTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK32C (282974)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76932
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
132307617 |
- |
STK32C |
NNNGGGCTCGTCCATGTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
48921245 |
- |
CCDC65 |
NNNGGGCTCGTCTATGTTGG |
NGG |
2 |
0.45 |
Tier I |
| 3 |
NC_000006.12 |
6 |
107634625 |
+ |
SOBP |
NNNGGGCTCGTCCAAGTCCG |
NGG |
2 |
0.2595 |
Tier I |
| 4 |
NC_000006.12 |
6 |
113859783 |
- |
MARCKS |
NNNGGGCTCCTCCTTGTCGG |
NGG |
2 |
0.2133 |
Tier I |
| 5 |
NC_000005.10 |
5 |
179817401 |
- |
SQSTM1 |
NNNGGGGTCGTCCATGCCGG |
NGG |
2 |
0.3322 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
126180090 |
- |
Thrap3 |
NNNGGGCTCGTCTATGTCAG |
NGG |
2 |
0.5 |
Tier I |
| 2 |
NC_000076.6 |
10 |
37136836 |
+ |
Marcks |
NNNGGGCTCCTCCTTGTCGG |
NGG |
2 |
0.2133 |
Tier I |
| 3 |
NC_000071.6 |
5 |
140074910 |
- |
Mad1l1 |
NNNGGGCTAGTCCATGTCAG |
NGG |
2 |
0.6122 |
Tier II |
| 4 |
NC_000073.6 |
7 |
63542564 |
+ |
Otud7a |
NNNGGGCTCGTCTATGTCAG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000078.6 |
12 |
104942093 |
- |
Syne3 |
NNNGGCCTCGTCCATGTCTG |
NGG |
2 |
0.4545 |
Tier II |
| 6 |
NC_000073.6 |
7 |
63542564 |
+ |
Gm5898 |
NNNGGGCTCGTCTATGTCAG |
NGG |
2 |
0.5 |
Tier III |
| 7 |
NC_000084.6 |
18 |
38056842 |
+ |
Gm30093 |
NNNGGGCTCGTCCATGCTGG |
NGG |
2 |
0.4538 |
Tier III |
| 8 |
NC_000084.6 |
18 |
38056842 |
+ |
Gm41701 |
NNNGGGCTCGTCCATGCTGG |
NGG |
2 |
0.4538 |
Tier III |
Other clones with same target sequence:
(none)
