Construct: sgRNA BRDN0001146379
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTCGAACTGGTCAACCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKK2 (10645)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76699
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
121255788 |
- |
CAMKK2 |
NNNTCGAACTGGTCAACCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
91480456 |
+ |
NFIL3 |
NNNTTGAACTGGTCAACTAA |
NGG |
2 |
0.4091 |
Tier II |
| 3 |
NC_000012.12 |
12 |
49705391 |
+ |
FMNL3 |
NNNACGAAATGGTCAACCAA |
NGG |
2 |
0.4082 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
82846666 |
+ |
Zdhhc21 |
NNNTCGAACTGGTCAAATAA |
NGG |
2 |
0.3 |
Tier II |
| 2 |
NC_000071.6 |
5 |
71594767 |
+ |
Gabra4 |
NNNTCGAGCTGGGCAACCAA |
NGG |
2 |
0.1913 |
Tier II |
| 3 |
NC_000076.6 |
10 |
71087781 |
- |
Bicc1 |
NNNTCGAACTGGTCAACCAC |
NGC |
2 |
0.0051 |
Tier II |
Other clones with same target sequence:
(none)
