Construct: sgRNA BRDN0001146390
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGTATCAGGATCCAACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GSK3B (2932)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77282
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
119916089 |
+ |
GSK3B |
NNNTATCAGGATCCAACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
140685123 |
+ |
HARS1 |
NNNCATCAGAATCCAACAAG |
NGG |
2 |
0.6039 |
Tier II |
| 3 |
NC_000004.12 |
4 |
7584122 |
- |
SORCS2 |
NNNTATTAGGATCCAACATG |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000017.11 |
17 |
69480597 |
- |
MAP2K6 |
NNNTTTCAGGATCCAAAAAG |
NGG |
2 |
0.1697 |
Tier II |
| 5 |
NC_000005.10 |
5 |
169648496 |
+ |
DOCK2 |
NNNTATCATGATCCAGCAAG |
NGG |
2 |
0.1026 |
Tier II |
| 6 |
NC_000003.12 |
3 |
196957331 |
+ |
PIGZ |
NNNCATCAGGATCCAACAAG |
NGC |
2 |
0.0144 |
Tier II |
| 7 |
NC_000003.12 |
3 |
59394997 |
- |
CFAP20DC-DT |
NNNTACCAGGATCCAACAAG |
NAG |
2 |
0.2357 |
Tier III |
| 8 |
NC_000004.12 |
4 |
158496584 |
+ |
LOC124900802 |
NNNTATCAGGATCCAACATC |
NGG |
2 |
0.2308 |
Tier III |
| 9 |
NC_000011.10 |
11 |
28950936 |
- |
LINC02742 |
NNNTATCTTGATCCAACAAG |
NGG |
2 |
0.2286 |
Tier III |
| 10 |
NC_000017.11 |
17 |
69480597 |
- |
LOC105371880 |
NNNTTTCAGGATCCAAAAAG |
NGG |
2 |
0.1697 |
Tier III |
| 11 |
NC_000002.12 |
2 |
35556329 |
- |
LOC107985866 |
NNNTTTCAGCATCCAACAAG |
NGG |
2 |
0.1455 |
Tier III |
| 12 |
NC_000001.11 |
1 |
60731341 |
+ |
LOC101926964 |
NNNTATCTGGATCCCACAAG |
NGG |
2 |
0.1169 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
38187897 |
- |
Gsk3b |
NNNTATCAGGATCCAACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
107979802 |
+ |
Uba7 |
NNNCATCAGGATCCAACAAG |
NGT |
2 |
0.0104 |
Tier I |
| 3 |
NC_000074.6 |
8 |
37537378 |
+ |
Sgcz |
NNNTCTCAGGACCCAACAAG |
NGG |
2 |
0.4737 |
Tier II |
| 4 |
NC_000071.6 |
5 |
114923922 |
- |
Oasl1 |
NNNTGTCAGGATCCAACTAG |
NGG |
2 |
0.36 |
Tier II |
| 5 |
NC_000081.6 |
15 |
64333297 |
+ |
Asap1 |
NNNTATCAGGAGCCAACATG |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000067.6 |
1 |
130839808 |
+ |
Pigr |
NNNTATCTGGATCCAACAAG |
NGA |
2 |
0.0298 |
Tier II |
Other clones with same target sequence:
(none)
