Construct: sgRNA BRDN0001146391
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCCCGGGGGTATCAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK1 (65125)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75622
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
879868 |
+ |
WNK1 |
NNNCCCGGGGGTATCAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
100736755 |
+ |
PAX2 |
NNNCCCGGGAGTGTCAACTG |
NGG |
2 |
0.6087 |
Tier II |
| 3 |
NC_000023.11 |
X |
44881924 |
- |
KDM6A |
NNNCGCGGGGGGATCAACTG |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000020.11 |
20 |
5002131 |
- |
SLC23A2 |
NNNCCCGGGGGCATCACCTG |
NGG |
2 |
0.1672 |
Tier II |
| 5 |
NC_000019.10 |
19 |
2677739 |
+ |
GNG7 |
NNNCCCGGGGGTCTCACCTG |
NGG |
2 |
0.0372 |
Tier II |
| 6 |
NC_000006.12 |
6 |
34671303 |
+ |
ILRUN |
NNNCGCGGGGGTATCAACTG |
NGT |
2 |
0.0097 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
96860679 |
- |
Pdzk1 |
NNNCCTGGGGGAATCAACTG |
NGG |
2 |
0.7429 |
Tier II |
| 2 |
NC_000075.6 |
9 |
68901804 |
+ |
Rora |
NNNCCCGTGGGTAACAACTG |
NGG |
2 |
0.3869 |
Tier II |
| 3 |
NC_000069.6 |
3 |
122324171 |
+ |
Gm40139 |
NNNCCCGAGGGTGTCAACTG |
NGG |
2 |
0.6522 |
Tier III |
| 4 |
NC_000075.6 |
9 |
68901804 |
+ |
Gm31262 |
NNNCCCGTGGGTAACAACTG |
NGG |
2 |
0.3869 |
Tier III |
Other clones with same target sequence:
(none)
