Construct: sgRNA BRDN0001146396
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGAGCATTCTGTGAGTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TWF2 (11344)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77342
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
52232005 |
+ |
TWF2 |
NNNGAGCATTCTGTGAGTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
3424806 |
- |
TRAPPC12 |
NNNGAGCATTCTGTAAGTCA |
NGG |
2 |
0.8824 |
Tier II |
| 3 |
NC_000003.12 |
3 |
188176443 |
- |
LPP |
NNNAAGCAGTCTGTGAGTCG |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000015.10 |
15 |
72266366 |
+ |
PARP6 |
NNNGAGCATTCTGAGAGTCT |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000019.10 |
19 |
34381313 |
- |
GPI |
NNNGAGCAGCCTGTGAGTCG |
NGG |
2 |
0.3302 |
Tier II |
| 6 |
NC_000002.12 |
2 |
237663254 |
+ |
LRRFIP1 |
NNNGAGCATTCTGTGAGATG |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000012.12 |
12 |
21648907 |
+ |
LDHB |
NNNGATCATTCTGTGAGTTG |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000004.12 |
4 |
814573 |
- |
CPLX1 |
NNNGAGGATGCTGTGAGTCG |
NGG |
2 |
0.2353 |
Tier II |
| 9 |
NC_000002.12 |
2 |
120928883 |
- |
GLI2 |
NNNGAGCATTCTGTCAGTCT |
NGG |
2 |
0.1909 |
Tier II |
| 10 |
NC_000007.14 |
7 |
158310384 |
+ |
PTPRN2 |
NNNGAGCATTCTGTGAGGCC |
NGG |
2 |
0.1429 |
Tier II |
| 11 |
NC_000022.11 |
22 |
26622045 |
+ |
CRYBA4 |
NNNGAGCATTCTGGGAGGCG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000016.10 |
16 |
2752324 |
- |
SRRM2-AS1 |
NNNGAGCATTCTGGGAGTTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
3162371 |
+ |
Scaf8 |
NNNAAGCATTCTGTGAGTCA |
NGG |
2 |
0.8438 |
Tier II |
| 2 |
NC_000068.7 |
2 |
40777199 |
+ |
Lrp1b |
NNNGAGAATTCTGTGAGTTG |
NGG |
2 |
0.3462 |
Tier II |
| 3 |
NC_000083.6 |
17 |
86218556 |
- |
Prkce |
NNNGAGCCTTCTGTGAGACG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000075.6 |
9 |
59623550 |
- |
Parp6 |
NNNGAGCATTCTGAGAGTCG |
NAG |
2 |
0.1605 |
Tier II |
| 5 |
NC_000067.6 |
1 |
42299292 |
+ |
Gm28175 |
NNNGTGCATTCTGTGAGTCG |
NGA |
2 |
0.0253 |
Tier III |
Other clones with same target sequence:
(none)
