Construct: sgRNA BRDN0001146397
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACACACCCGTATGGCAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA2 (1969)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76367
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
16150901 |
- |
EPHA2 |
NNNACACCCGTATGGCAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
2050670 |
+ |
CACNA1C |
NNNACACCAGAATGGCAAAG |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000011.10 |
11 |
2306105 |
- |
TSPAN32 |
NNNACACCCGTATGGCAACA |
NGG |
2 |
0.194 |
Tier II |
| 4 |
NC_000006.12 |
6 |
40569541 |
+ |
LRFN2 |
NNNACACCCGTTTGGCATAG |
NGG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000010.11 |
10 |
34551202 |
+ |
PARD3 |
NNNACATCCGTATGTCAAAG |
NGG |
2 |
0.1161 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
141306577 |
+ |
Epha2 |
NNNGCACCCCTATGGCAAAG |
NGG |
2 |
0.25 |
Tier I |
| 2 |
NC_000083.6 |
17 |
5960786 |
- |
Synj2 |
NNNACACCCAAATGGCAAAG |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000079.6 |
13 |
34646012 |
- |
Pxdc1 |
NNNACACCTGTGTGGCAAAG |
NGG |
2 |
0.6319 |
Tier II |
| 4 |
NC_000069.6 |
3 |
36014239 |
+ |
Ccdc144b |
NNNATACACGTATGGCAAAG |
NGG |
2 |
0.4136 |
Tier II |
| 5 |
NC_000075.6 |
9 |
37232834 |
- |
Slc37a2 |
NNNACACCCGCAGGGCAAAG |
NGG |
2 |
0.1739 |
Tier II |
| 6 |
NC_000073.6 |
7 |
141487087 |
+ |
Tspan4 |
NNNACACCCGGATGGCAGAG |
NGG |
2 |
0.16 |
Tier II |
| 7 |
NC_000077.6 |
11 |
59719332 |
- |
Mprip |
NNNACACCCTTATGTCAAAG |
NGG |
2 |
0.1161 |
Tier II |
Other clones with same target sequence:
(none)
