Construct: sgRNA BRDN0001146399
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAACCTGCAACAATCACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTN (7273)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76853
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
178731189 |
- |
TTN |
NNNACCTGCAACAATCACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
25290780 |
+ |
DNMT3A |
NNNACCTGCAGCGATCACCG |
NGG |
2 |
0.4239 |
Tier II |
| 3 |
NC_000012.12 |
12 |
112587902 |
- |
RPH3A |
NNNACCTCCAACATTCACCG |
NGG |
2 |
0.3282 |
Tier II |
| 4 |
NC_000005.10 |
5 |
144438600 |
+ |
KCTD16 |
NNNACCTGCATCAATCACTG |
NGG |
2 |
0.142 |
Tier II |
| 5 |
NC_000008.11 |
8 |
1475514 |
- |
DLGAP2 |
NNNGCCTGCAACAATCAGCG |
NGG |
2 |
0.0833 |
Tier II |
| 6 |
NC_000001.11 |
1 |
3431954 |
- |
PRDM16 |
NNNACCTGCACCAATGACCG |
NGG |
2 |
0.0615 |
Tier II |
| 7 |
NC_000016.10 |
16 |
81641301 |
+ |
CMIP |
NNNACCTGCAACAAACACCG |
NGA |
2 |
0.0402 |
Tier II |
| 8 |
NC_000020.11 |
20 |
11356238 |
+ |
LOC105372529 |
NNNATCTGCAACAATCACCT |
NGG |
2 |
0.4455 |
Tier III |
| 9 |
NC_000007.14 |
7 |
24425660 |
- |
LOC107986777 |
NNNACCTGCAACAATCACCA |
NAG |
2 |
0.2431 |
Tier III |
| 10 |
NC_000018.10 |
18 |
26688285 |
+ |
PCAT18 |
NNNACCTGCAAGAATCACCC |
NGG |
2 |
0.1905 |
Tier III |
| 11 |
NC_000018.10 |
18 |
26688285 |
+ |
LOC102725227 |
NNNACCTGCAAGAATCACCC |
NGG |
2 |
0.1905 |
Tier III |
| 12 |
NC_000016.10 |
16 |
81641301 |
+ |
LOC105371362 |
NNNACCTGCAACAAACACCG |
NGA |
2 |
0.0402 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
145295125 |
+ |
Slc24a3 |
NNNACCTACAACAATCACCT |
NGG |
2 |
0.7 |
Tier II |
| 2 |
NC_000076.6 |
10 |
121797191 |
- |
Srgap1 |
NNNACATGCAACAGTCACCG |
NGG |
2 |
0.4333 |
Tier II |
| 3 |
NC_000074.6 |
8 |
9395008 |
+ |
Fam155a |
NNNACCTGCAACAATGACCA |
NGG |
2 |
0.1442 |
Tier II |
| 4 |
NC_000075.6 |
9 |
31884716 |
- |
Barx2 |
NNNACCTGCAACAATGACCT |
NGG |
2 |
0.1077 |
Tier II |
| 5 |
NC_000076.6 |
10 |
86315701 |
- |
Timp3 |
NNNACCTGCAACAAGCACAG |
NGG |
2 |
0.0214 |
Tier II |
| 6 |
NC_000076.6 |
10 |
86315701 |
- |
Syn3 |
NNNACCTGCAACAAGCACAG |
NGG |
2 |
0.0214 |
Tier II |
| 7 |
NC_000076.6 |
10 |
121797191 |
- |
Gm46183 |
NNNACATGCAACAGTCACCG |
NGG |
2 |
0.4333 |
Tier III |
| 8 |
NC_000072.6 |
6 |
135660048 |
- |
4930425L21Rik |
NNNACCTTCATCAATCACCG |
NGG |
2 |
0.1923 |
Tier III |
Other clones with same target sequence:
(none)
