Construct: sgRNA BRDN0001146401
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGAGGCTGAGTTAGTGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK10 (152110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75633
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000082.6 |
16 |
32829519 |
+ |
Rubcn |
NNNAGGCTGAATTAGTGACA |
NGG |
1 |
1.0 |
Tier II |
2 |
NC_000077.6 |
11 |
33689473 |
+ |
Kcnip1 |
NNNAGGTGGAGTTAGTGACA |
NGG |
2 |
0.5958 |
Tier II |
3 |
NC_000072.6 |
6 |
34724697 |
- |
Cald1 |
NNNTGGCAGAGTTAGTGACA |
NGG |
2 |
0.5091 |
Tier II |
4 |
NC_000068.7 |
2 |
174341439 |
- |
Gnas |
NNNAGGCTGGGTTAGAGACA |
NGG |
2 |
0.5051 |
Tier II |
5 |
NC_000080.6 |
14 |
34071981 |
- |
A630023A22Rik |
NNNGGGCTGAGTCAGTGACA |
NGG |
2 |
0.4934 |
Tier II |
6 |
NC_000080.6 |
14 |
34071981 |
- |
Antxrl |
NNNGGGCTGAGTCAGTGACA |
NGG |
2 |
0.4934 |
Tier II |
7 |
NC_000085.6 |
19 |
43968103 |
+ |
Cpn1 |
NNNAGGCTTAGTTAGAGACA |
NGG |
2 |
0.4848 |
Tier II |
8 |
NC_000080.6 |
14 |
122030682 |
+ |
Timm8a2 |
NNNAGGCTCTGTTAGTGACA |
NGG |
2 |
0.4751 |
Tier II |
9 |
NC_000076.6 |
10 |
76576744 |
+ |
Ftcd |
NNNAGGCTGAGCTGGTGACA |
NGG |
2 |
0.4421 |
Tier II |
10 |
NC_000072.6 |
6 |
90125108 |
- |
Vmn1r51 |
NNNAGGCTGTGTTAGTGTCA |
NGG |
2 |
0.4412 |
Tier II |
11 |
NC_000081.6 |
15 |
26347646 |
+ |
Marchf11 |
NNNAGGCACAGTTAGTGACA |
NGG |
2 |
0.4308 |
Tier II |
12 |
NC_000082.6 |
16 |
42063111 |
+ |
Lsamp |
NNNAGGTTGAGTTGGTGACA |
NGG |
2 |
0.3792 |
Tier II |
13 |
NC_000082.6 |
16 |
42063111 |
+ |
Gm52229 |
NNNAGGTTGAGTTGGTGACA |
NGG |
2 |
0.3792 |
Tier II |
14 |
NC_000068.7 |
2 |
59742054 |
+ |
Tanc1 |
NNNAGGCAGAGTTGGTGACA |
NGG |
2 |
0.3733 |
Tier II |
15 |
NC_000086.7 |
X |
36902705 |
- |
Nkrf |
NNNAGGTTGAGTTAGGGACA |
NGG |
2 |
0.2813 |
Tier II |
16 |
NC_000086.7 |
X |
103361370 |
- |
Chic1 |
NNNAGGCTGGGTTGGTGACA |
NGG |
2 |
0.2593 |
Tier II |
17 |
NC_000079.6 |
13 |
43254950 |
+ |
Gfod1 |
NNNAGGCTGAGCTAGTGACA |
NAG |
2 |
0.2456 |
Tier II |
18 |
NC_000083.6 |
17 |
26607872 |
+ |
Ergic1 |
NNNAGCCTGAGTTAGGGACA |
NGG |
2 |
0.236 |
Tier II |
19 |
NC_000071.6 |
5 |
14591539 |
+ |
Pclo |
NNNAGGATGAGTGAGTGACA |
NGG |
2 |
0.1957 |
Tier II |
20 |
NC_000073.6 |
7 |
55852585 |
+ |
Cyfip1 |
NNNAGGCTGAGTAAGTCACA |
NGG |
2 |
0.1629 |
Tier II |
21 |
NC_000074.6 |
8 |
118287190 |
+ |
Cdh13 |
NNNAGGCTGAGTAAGTGACC |
NGG |
2 |
0.1573 |
Tier II |
22 |
NC_000070.6 |
4 |
32766720 |
- |
Mdn1 |
NNNAGGCTCAGTTAGTTACA |
NGG |
2 |
0.1346 |
Tier II |
23 |
NC_000080.6 |
14 |
12222461 |
+ |
Ptprg |
NNNAGGCTGAGGTAGTTACA |
NGG |
2 |
0.125 |
Tier II |
24 |
NC_000071.6 |
5 |
128347615 |
+ |
Tmem132d |
NNNAGGCTGAGTTTGTGACC |
NGG |
2 |
0.1212 |
Tier II |
25 |
NC_000077.6 |
11 |
67214441 |
- |
Myh1 |
NNNAGGCTGAGTTGGTGACA |
NAG |
2 |
0.121 |
Tier II |
26 |
NC_000069.6 |
3 |
94874335 |
+ |
Pogz |
NNNAGGCTGAGTTAGAGAGA |
NGG |
2 |
0.1136 |
Tier II |
27 |
NC_000080.6 |
14 |
117756601 |
- |
Gpc6 |
NNNAGGCTGAGTTACTGGCA |
NGG |
2 |
0.1091 |
Tier II |
28 |
NC_000078.6 |
12 |
108977767 |
- |
Wdr25 |
NNNAGGCTGAGTTAGTGAGG |
NGG |
2 |
0.0956 |
Tier II |
29 |
NC_000078.6 |
12 |
34388236 |
- |
Hdac9 |
NNNGGGCTGAGTTAGTGAGA |
NGG |
2 |
0.0781 |
Tier II |
30 |
NC_000075.6 |
9 |
70474399 |
- |
Rnf111 |
NNNAGGCTGAGGTAGTGAGA |
NGG |
2 |
0.0625 |
Tier II |
31 |
NC_000072.6 |
6 |
118610619 |
+ |
Cacna1c |
NNNAGGCTGAGTCAGTGACA |
NTG |
2 |
0.0308 |
Tier II |
32 |
NC_000079.6 |
13 |
12251522 |
- |
Mtr |
NNNAGGCTGAGTTATTGAGA |
NGG |
2 |
0.0179 |
Tier II |
33 |
NC_000071.6 |
5 |
136315864 |
+ |
Cux1 |
NNNAGGCTCAGTTAGTGACA |
NGC |
2 |
0.012 |
Tier II |
34 |
NC_000067.6 |
1 |
110129078 |
+ |
Cdh7 |
NNNAGGCTGAGTTAGTGAAA |
NGC |
2 |
0.0095 |
Tier II |
35 |
NC_000072.6 |
6 |
50503189 |
+ |
Gm40372 |
NNNAGGCTGAGTAAGTAACA |
NGG |
2 |
0.6462 |
Tier III |
36 |
NC_000077.6 |
11 |
33689473 |
+ |
Gm12119 |
NNNAGGTGGAGTTAGTGACA |
NGG |
2 |
0.5958 |
Tier III |
37 |
NC_000072.6 |
6 |
34724697 |
- |
Gm38787 |
NNNTGGCAGAGTTAGTGACA |
NGG |
2 |
0.5091 |
Tier III |
38 |
NC_000079.6 |
13 |
43254950 |
+ |
LOC115488162 |
NNNAGGCTGAGCTAGTGACA |
NAG |
2 |
0.2456 |
Tier III |
39 |
NC_000073.6 |
7 |
26950799 |
- |
Cyp2a21-ps |
NNNAGGATGAGTGAGTGACA |
NGG |
2 |
0.1957 |
Tier III |
40 |
NC_000074.6 |
8 |
118287190 |
+ |
Gm51555 |
NNNAGGCTGAGTAAGTGACC |
NGG |
2 |
0.1573 |
Tier III |
41 |
NC_000079.6 |
13 |
71582372 |
- |
1700112M02Rik |
NNNTGGCTGAGTTAGTGACA |
NCG |
2 |
0.0682 |
Tier III |
42 |
NC_000072.6 |
6 |
41107494 |
- |
Tcrb |
NNNAGACTGAGTTAGTGACA |
NTG |
2 |
0.039 |
Tier III |
43 |
NC_000075.6 |
9 |
100313097 |
- |
Gm28167 |
NNNAGGCTGAGTGAGTGACA |
NGT |
2 |
0.0042 |
Tier III |
Other clones with same target sequence:
(none)