Construct: sgRNA BRDN0001146401

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAGAGGCTGAGTTAGTGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK10 (152110)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75633

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 27162519 - NEK10 NNNAGGCTGAGTTAGTGACA NGG 0 1.0 Tier I
2 NC_000001.11 1 93526052 + FNBP1L NNNAGGTTGAATTAGTGACA NGG 2 0.8125 Tier II
3 NC_000008.11 8 55766314 + TMEM68 NNNAGGCTGAGAAAGTGACA NGG 2 0.5538 Tier II
4 NC_000002.12 2 113452406 - ZNG1B NNNAGGCCCAGTTAGTGACA NGG 2 0.5385 Tier II
5 NC_000009.12 9 164771 + ZNG1A NNNAGGCCCAGTTAGTGACA NGG 2 0.5385 Tier II
6 NC_000009.12 9 41175086 + ZNG1F NNNAGGCCCAGTTAGTGACA NGG 2 0.5385 Tier II
7 NC_000009.12 9 65690234 - ZNG1E NNNAGGCCCAGTTAGTGACA NGG 2 0.5385 Tier II
8 NC_000009.12 9 68256190 - ZNG1C NNNAGGCCCAGTTAGTGACA NGG 2 0.5385 Tier II
9 NC_000017.11 17 80194491 - CARD14 NNNAGGTTGAGTTAGTGACT NGG 2 0.4875 Tier II
10 NC_000009.12 9 9360492 + PTPRD NNNAGGCTAAGTTTGTGACA NGG 2 0.3429 Tier II
11 NC_000016.10 16 72859276 - ZFHX3 NNNTGGCTGAGTTGGTGACA NGG 2 0.297 Tier II
12 NC_000009.12 9 36617901 + MELK NNNGGGCTGAGTTAGGGACA NGG 2 0.2163 Tier II
13 NC_000005.10 5 138253109 - GFRA3 NNNAGGCTGAGGTAGTGAAA NGG 2 0.2143 Tier II
14 NC_000016.10 16 81644332 + CMIP NNNAGGCTGATTTTGTGACA NGG 2 0.2051 Tier II
15 NC_000004.12 4 122327181 + BLTP1 NNNATCCTGAGTTAGTGACA NGG 2 0.2045 Tier II
16 NC_000010.11 10 47666950 - ANXA8 NNNAGGTTGAGTTAGTCACA NGG 2 0.1912 Tier II
17 NC_000020.11 20 61268863 - CDH4 NNNAGGCTGAGTAAGTGACA NAG 2 0.1795 Tier II
18 NC_000015.10 15 41566297 + TYRO3 NNNAGCCTGAGTGAGTGACA NGG 2 0.1779 Tier II
19 NC_000002.12 2 205592905 - PARD3B NNNAGGCTGAGTCAGTGCCA NGG 2 0.1504 Tier II
20 NC_000009.12 9 114390046 - AKNA NNNAGGGTGAGTGAGTGACA NGG 2 0.1228 Tier II
21 NC_000010.11 10 71623180 + CDH23 NNNAGGGTGAGTGAGTGACA NGG 2 0.1228 Tier II
22 NC_000016.10 16 66972758 + CES3 NNNAGGGTGAGTGAGTGACA NGG 2 0.1228 Tier II
23 NC_000020.11 20 9398264 + PLCB4 NNNAGGGTGAGTGAGTGACA NGG 2 0.1228 Tier II
24 NC_000006.12 6 143245022 - AIG1 NNNATGCTGATTTAGTGACA NGG 2 0.1154 Tier II
25 NC_000012.12 12 12511836 + DUSP16 NNNAGGCTGAGTGAGTGAAA NGG 2 0.1118 Tier II
26 NC_000014.9 14 104988264 - CLBA1 NNNAGGCTGAGTTAGTGTGA NGG 2 0.0625 Tier II
27 NC_000008.11 8 122796329 - ZHX2 NNNAGGCTGTGTTAGTGACA NGA 2 0.0613 Tier II
28 NC_000003.12 3 108303946 + HHLA2 NNNAGGCTGAGTTATTGGCA NGG 2 0.0571 Tier II
29 NC_000003.12 3 47303345 + KLHL18 NNNAGGCTGAGTTAGAGACA NTG 2 0.0354 Tier II
30 NC_000008.11 8 11456688 + FAM167A NNNGGGCTGAGTTAGTGACA NGT 2 0.0101 Tier II
31 NC_000012.12 12 120234942 - PXN NNNAGGCTGAGTTAGTGACA NCC 2 0.0 Tier II
32 NC_000009.12 9 118596578 - LOC105376249 NNNAGGCTGGGTTAGTAACA NGG 2 0.5185 Tier III
33 NC_000016.10 16 81644332 + LOC105371362 NNNAGGCTGATTTTGTGACA NGG 2 0.2051 Tier III
34 NC_000015.10 15 66609547 - LINC01169 NNNTGGCTGAGTGAGTGACA NGG 2 0.166 Tier III
35 NC_000001.11 1 85622173 + LOC112268231 NNNGGGCTGAGTGAGTGACA NGG 2 0.163 Tier III
36 NC_000009.12 9 114390046 - LOC124902253 NNNAGGGTGAGTGAGTGACA NGG 2 0.1228 Tier III
37 NC_000014.9 14 40973950 + LINC02315 NNNAGGCTAAGTTAGTGACA NTG 2 0.025 Tier III
38 NC_000015.10 15 35088888 - LOC105370765 NNNAGGCTGAGTTAGTGACA NCC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 32829519 + Rubcn NNNAGGCTGAATTAGTGACA NGG 1 1.0 Tier II
2 NC_000077.6 11 33689473 + Kcnip1 NNNAGGTGGAGTTAGTGACA NGG 2 0.5958 Tier II
3 NC_000072.6 6 34724697 - Cald1 NNNTGGCAGAGTTAGTGACA NGG 2 0.5091 Tier II
4 NC_000068.7 2 174341439 - Gnas NNNAGGCTGGGTTAGAGACA NGG 2 0.5051 Tier II
5 NC_000080.6 14 34071981 - A630023A22Rik NNNGGGCTGAGTCAGTGACA NGG 2 0.4934 Tier II
6 NC_000080.6 14 34071981 - Antxrl NNNGGGCTGAGTCAGTGACA NGG 2 0.4934 Tier II
7 NC_000085.6 19 43968103 + Cpn1 NNNAGGCTTAGTTAGAGACA NGG 2 0.4848 Tier II
8 NC_000080.6 14 122030682 + Timm8a2 NNNAGGCTCTGTTAGTGACA NGG 2 0.4751 Tier II
9 NC_000076.6 10 76576744 + Ftcd NNNAGGCTGAGCTGGTGACA NGG 2 0.4421 Tier II
10 NC_000072.6 6 90125108 - Vmn1r51 NNNAGGCTGTGTTAGTGTCA NGG 2 0.4412 Tier II
11 NC_000081.6 15 26347646 + Marchf11 NNNAGGCACAGTTAGTGACA NGG 2 0.4308 Tier II
12 NC_000082.6 16 42063111 + Lsamp NNNAGGTTGAGTTGGTGACA NGG 2 0.3792 Tier II
13 NC_000082.6 16 42063111 + Gm52229 NNNAGGTTGAGTTGGTGACA NGG 2 0.3792 Tier II
14 NC_000068.7 2 59742054 + Tanc1 NNNAGGCAGAGTTGGTGACA NGG 2 0.3733 Tier II
15 NC_000086.7 X 36902705 - Nkrf NNNAGGTTGAGTTAGGGACA NGG 2 0.2813 Tier II
16 NC_000086.7 X 103361370 - Chic1 NNNAGGCTGGGTTGGTGACA NGG 2 0.2593 Tier II
17 NC_000079.6 13 43254950 + Gfod1 NNNAGGCTGAGCTAGTGACA NAG 2 0.2456 Tier II
18 NC_000083.6 17 26607872 + Ergic1 NNNAGCCTGAGTTAGGGACA NGG 2 0.236 Tier II
19 NC_000071.6 5 14591539 + Pclo NNNAGGATGAGTGAGTGACA NGG 2 0.1957 Tier II
20 NC_000073.6 7 55852585 + Cyfip1 NNNAGGCTGAGTAAGTCACA NGG 2 0.1629 Tier II
21 NC_000074.6 8 118287190 + Cdh13 NNNAGGCTGAGTAAGTGACC NGG 2 0.1573 Tier II
22 NC_000070.6 4 32766720 - Mdn1 NNNAGGCTCAGTTAGTTACA NGG 2 0.1346 Tier II
23 NC_000080.6 14 12222461 + Ptprg NNNAGGCTGAGGTAGTTACA NGG 2 0.125 Tier II
24 NC_000071.6 5 128347615 + Tmem132d NNNAGGCTGAGTTTGTGACC NGG 2 0.1212 Tier II
25 NC_000077.6 11 67214441 - Myh1 NNNAGGCTGAGTTGGTGACA NAG 2 0.121 Tier II
26 NC_000069.6 3 94874335 + Pogz NNNAGGCTGAGTTAGAGAGA NGG 2 0.1136 Tier II
27 NC_000080.6 14 117756601 - Gpc6 NNNAGGCTGAGTTACTGGCA NGG 2 0.1091 Tier II
28 NC_000078.6 12 108977767 - Wdr25 NNNAGGCTGAGTTAGTGAGG NGG 2 0.0956 Tier II
29 NC_000078.6 12 34388236 - Hdac9 NNNGGGCTGAGTTAGTGAGA NGG 2 0.0781 Tier II
30 NC_000075.6 9 70474399 - Rnf111 NNNAGGCTGAGGTAGTGAGA NGG 2 0.0625 Tier II
31 NC_000072.6 6 118610619 + Cacna1c NNNAGGCTGAGTCAGTGACA NTG 2 0.0308 Tier II
32 NC_000079.6 13 12251522 - Mtr NNNAGGCTGAGTTATTGAGA NGG 2 0.0179 Tier II
33 NC_000071.6 5 136315864 + Cux1 NNNAGGCTCAGTTAGTGACA NGC 2 0.012 Tier II
34 NC_000067.6 1 110129078 + Cdh7 NNNAGGCTGAGTTAGTGAAA NGC 2 0.0095 Tier II
35 NC_000072.6 6 50503189 + Gm40372 NNNAGGCTGAGTAAGTAACA NGG 2 0.6462 Tier III
36 NC_000077.6 11 33689473 + Gm12119 NNNAGGTGGAGTTAGTGACA NGG 2 0.5958 Tier III
37 NC_000072.6 6 34724697 - Gm38787 NNNTGGCAGAGTTAGTGACA NGG 2 0.5091 Tier III
38 NC_000079.6 13 43254950 + LOC115488162 NNNAGGCTGAGCTAGTGACA NAG 2 0.2456 Tier III
39 NC_000073.6 7 26950799 - Cyp2a21-ps NNNAGGATGAGTGAGTGACA NGG 2 0.1957 Tier III
40 NC_000074.6 8 118287190 + Gm51555 NNNAGGCTGAGTAAGTGACC NGG 2 0.1573 Tier III
41 NC_000079.6 13 71582372 - 1700112M02Rik NNNTGGCTGAGTTAGTGACA NCG 2 0.0682 Tier III
42 NC_000072.6 6 41107494 - Tcrb NNNAGACTGAGTTAGTGACA NTG 2 0.039 Tier III
43 NC_000075.6 9 100313097 - Gm28167 NNNAGGCTGAGTGAGTGACA NGT 2 0.0042 Tier III
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Other clones with same target sequence:

(none)