Construct: sgRNA BRDN0001146403

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTACCTCTGAAGATACTCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK32B (55351)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76373

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 5331363 - STK32B NNNCCTCTGAAGATACTCCA NGG 0 1.0 Tier I
2 NC_000002.12 2 233139871 - INPP5D NNNCCTCTGCAGAGACTCCA NGG 2 0.0 Tier I
3 NC_000015.10 15 31693946 + OTUD7A NNNCCTCTGAAGATGCTCCA NGG 1 0.65 Tier II
4 NC_000010.11 10 104813712 + SORCS3 NNNCCACTGAAGATGCTCCA NGG 2 0.5633 Tier II
5 NC_000015.10 15 74333378 + CCDC33 NNNCCTCTGAAGATGCTTCA NGG 2 0.4179 Tier II
6 NC_000001.11 1 156194513 - SLC25A44 NNNCCTCTCAAGATACTCCG NGG 2 0.4118 Tier II
7 NC_000008.11 8 31645926 + NRG1 NNNCCTATGAAGATACTACA NGG 2 0.4038 Tier II
8 NC_000003.12 3 68012893 + TAFA1 NNNCTTCTGAAGATACTCCT NGG 2 0.3818 Tier II
9 NC_000009.12 9 28889044 - LINGO2 NNNCTGCTGAAGATACTCCA NGG 2 0.3636 Tier II
10 NC_000005.10 5 15792240 - FBXL7 NNNCCTGGGAAGATACTCCA NGG 2 0.3451 Tier II
11 NC_000001.11 1 241296036 - RGS7 NNNCTTCTCAAGATACTCCA NGG 2 0.3427 Tier II
12 NC_000002.12 2 226789245 - IRS1 NNNCCTCTTAAGAAACTCCA NGG 2 0.3302 Tier II
13 NC_000006.12 6 147517716 - SAMD5 NNNCCACTGAAGATACTCCA NAG 2 0.2247 Tier II
14 NC_000003.12 3 188679045 - LPP NNNCCTCTGAAGATACCCCA NAG 2 0.183 Tier II
15 NC_000002.12 2 219334299 - RESP18 NNNCCTCTGAAGATACTTCA NAG 2 0.1667 Tier II
16 NC_000004.12 4 99918636 + DNAJB14 NNNCCTCTGAAGACACTCAA NGG 2 0.1224 Tier II
17 NC_000020.11 20 24634840 - SYNDIG1 NNNCCACTGAAGATACTCCA NGA 2 0.0602 Tier II
18 NC_000007.14 7 855345 - SUN1 NNNCCTCTGAAGACACTGCA NGG 2 0.0381 Tier II
19 NC_000015.10 15 30711754 - LOC100288637 NNNCCTCTGAAGATGCTCCA NGG 1 0.65 Tier III
20 NC_000001.11 1 193834233 + LOC124904475 NNNCCTCTGAAGAAACTCCA NGG 1 0.619 Tier III
21 NC_000005.10 5 135320918 + PITX1-AS1 NNNCGTCTGAAGATGCTCCA NGG 2 0.39 Tier III
22 NC_000017.11 17 52460822 - LINC01982 NNNCCTTTGAAGATACTGCA NGG 2 0.1083 Tier III
23 NC_000004.12 4 96674978 + LINC02267 NNNCCTCTGAAGATCCTCCA NAG 2 0.0707 Tier III
24 NC_000015.10 15 61555202 + LOC107984782 NNNCCTTTGAAGAGACTCCA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 87676312 - Inpp5d NNNCCTCTGCAGAGACTCCA NGG 2 0.0 Tier I
2 NC_000077.6 11 81428459 - Asic2 NNNCCACAGAAGATACTCCA NGG 2 0.6933 Tier II
3 NC_000068.7 2 40813690 - Lrp1b NNNACTTTGAAGATACTCCA NGG 2 0.6842 Tier II
4 NC_000072.6 6 136747670 - Gucy2c NNNCCTCAGGAGATACTCCA NGG 2 0.4444 Tier II
5 NC_000072.6 6 32147423 + Plxna4 NNNCCTCTGAACAAACTCCA NGG 2 0.3277 Tier II
6 NC_000071.6 5 31691498 - Rbks NNNCGTCTGAAGATACACCA NGG 2 0.32 Tier II
7 NC_000075.6 9 58095194 - Stra6 NNNCCTCTGAACATACACCA NGG 2 0.2824 Tier II
8 NC_000075.6 9 58095194 - Ccdc33 NNNCCTCTGAACATACACCA NGG 2 0.2824 Tier II
9 NC_000070.6 4 151282114 - Camta1 NNNCCTCTGAAGAAACTCAA NGG 2 0.2653 Tier II
10 NC_000075.6 9 62783042 + Itga11 NNNCCTCTGAAGAAACTCAA NGG 2 0.2653 Tier II
11 NC_000078.6 12 104748490 + Dicer1 NNNACTCTGAAGATATTCCA NGG 2 0.2591 Tier II
12 NC_000077.6 11 50777306 - Adamts2 NNNCCTCTGCAGATACTACA NGG 2 0.1795 Tier II
13 NC_000074.6 8 83185395 - Tbc1d9 NNNCCTCTGTAGATTCTCCA NGG 2 0.1765 Tier II
14 NC_000080.6 14 124591401 - Fgf14 NNNCCTCAGAAGATAGTCCA NGG 2 0.1231 Tier II
15 NC_000073.6 7 40911903 + Vstm2b NNNCCTCTGAAGACACTCAA NGG 2 0.1224 Tier II
16 NC_000072.6 6 85291045 - Sfxn5 NNNTCTCTGAAGATACTGCA NGG 2 0.1067 Tier II
17 NC_000077.6 11 113280960 - Slc39a11 NNNCCTCTGAAGACACGCCA NGG 2 0.0336 Tier II
18 NC_000082.6 16 41262517 - Lsamp NNNCCTCTGAAGATAATCCA NGC 2 0.0222 Tier II
19 NC_000086.7 X 139605295 + Rnf128 NNNCCTCTGTAGATACTCCA NGT 2 0.0142 Tier II
20 NC_000071.6 5 148514529 + Ubl3 NNNCCTCTGAAGATTCTCCA NTG 2 0.0078 Tier II
21 NC_000076.6 10 43978428 + Crybg1 NNNCCTCTGAAGATACTGCA NTG 2 0.0052 Tier II
22 NC_000080.6 14 29285720 - Cacna2d3 NNNCCTCTGAAGAGACTGCA NGG 2 0.0 Tier II
23 NC_000084.6 18 62980047 - Napg NNNCCTCTGAGGAGACTCCA NGG 2 0.0 Tier II
24 NC_000078.6 12 111231665 - Traf3 NNNGCTCTGAAGAGACTCCA NGG 2 0.0 Tier II
25 NC_000078.6 12 11064057 - 7420701I03Rik NNNCCTCAGAAGATACTCCA NAG 2 0.2074 Tier III
26 NC_000077.6 11 34040452 + 4930469K13Rik NNNCCTCTGATGGTACTCCA NGG 2 0.2007 Tier III
27 NC_000067.6 1 51594962 + Gm17767 NNNGCTCTGAAGATCCTCCA NGG 2 0.1364 Tier III
28 NC_000081.6 15 87897519 - Gm35599 NNNCCTCTGCAGATCCTCCA NGG 2 0.0909 Tier III
29 NC_000076.6 10 43978428 + Gm40636 NNNCCTCTGAAGATACTGCA NTG 2 0.0052 Tier III
Download CSV

Other clones with same target sequence:

(none)