Construct: sgRNA BRDN0001146404
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCCAAGGAAGTATCGCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAN3 (255967)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77423
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28197200 |
+ |
PAN3 |
NNNCAAGGAAGTATCGCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
16029971 |
- |
MACROD2 |
NNNCAAGGAAGCATCACCTG |
NGG |
2 |
0.9474 |
Tier II |
| 3 |
NC_000019.10 |
19 |
33433680 |
- |
PEPD |
NNNTAAGGAAGTATCGCCTG |
NGC |
2 |
0.0178 |
Tier II |
| 4 |
NC_000002.12 |
2 |
137035729 |
- |
THSD7B |
NNNCAAGGAAGTATTGCCTG |
NGA |
2 |
0.0046 |
Tier II |
| 5 |
NC_000005.10 |
5 |
164650216 |
+ |
LINC03000 |
NNNCAAGGCAGGATCGCCTG |
NGG |
2 |
0.2857 |
Tier III |
| 6 |
NC_000005.10 |
5 |
124990905 |
+ |
LOC105379158 |
NNNCAAGGAAGAATTGCCTG |
NGG |
2 |
0.0533 |
Tier III |
| 7 |
NC_000001.11 |
1 |
223691794 |
- |
LOC105373281 |
NNNCAAGGAAGGATTGCCTG |
NGG |
2 |
0.0333 |
Tier III |
| 8 |
NC_000019.10 |
19 |
46668067 |
- |
DACT3-AS1 |
NNNCAAGGAAGGATTGCCTG |
NGG |
2 |
0.0333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
147466951 |
+ |
Pan3 |
NNNCAAGGAAGTATCGCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
106816680 |
- |
Olfr701 |
NNNCAAGGAAGTATCACATG |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000079.6 |
13 |
109469315 |
- |
Pde4d |
NNNGAAGGAAGTATCGCCTG |
NGG |
1 |
0.5 |
Tier II |
| 4 |
NC_000080.6 |
14 |
76426151 |
+ |
Tsc22d1 |
NNNCAGGGAAGTACCGCCTG |
NGG |
2 |
0.2041 |
Tier II |
| 5 |
NC_000073.6 |
7 |
100372679 |
+ |
C2cd3 |
NNNAAAGGAAGTAGCGCCTG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000070.6 |
4 |
129298380 |
+ |
Sync |
NNNCAAGGAAGTAGCGCCTG |
NAG |
2 |
0.0 |
Tier II |
| 7 |
NC_000072.6 |
6 |
53262615 |
+ |
Gm33348 |
NNNCGATGAAGTATCGCCTG |
NGG |
2 |
0.4114 |
Tier III |
Other clones with same target sequence:
(none)
